Chapter 3 Flashcards
What are chromosomes?
The structures within living cells that contain the genetic material. Genes are physically located within the structure of chromosomes. Biochemically, chromosomes contain a very long segment of DNA, which is the genetic material, and protein, which are bound to the DNA and provide it with an organized structure.
What is a chromatin?
The association between DNA and proteins that is found within chromosomes.
Bacteria and achaea are referred to as what?
Prokaryotes
What are prokaryotes?
Another name for bacteria and archaea. The term refers to the fact that the chromosomes are not contained within a separate nucleus of the cell.
Prokaryotes usually have a _________ in a region of the cytoplasm called the _________.
Single type of circular chromosome in a region of the cytoplasm called the nucleoid.
What is the nucleoid?
A darkly staining region that contains the genetic material of mitochondria, chloroplasts, or bacteria.
In bacteria, the cytoplasm is enclosed by what?
A plasma membrane that regulates the uptake of nutrients and the excretion of waste products.
What’s outside the plasma membrane in bacteria?
A rigid cell wall that protects the cell from breakage.
Eukaryotic cells exhibit compartmentalization. Define compartmentalization.
Cells have membrane-bound compartments.
What are eukaryotes?
Have a defining feature that their cells contain nuclei bounded by cell membranes. Some simple eukaryotic species are single-clled protists and yeast; more complex multicellular species include fungi, plants and animals.
The compartment of eukaryotic species form what?
Membrane-bound organelles.
What are organelles?
Large specialized structure within a cell, which is often surrounded by a single or double membrane.
Lysosomes play a role in what?
The degradation of macromolecules
The endoplasmic reticulum and golgi body play a role in what?
In protein modification and trafficking.
What is the nucleus?
A membrane-bound organelle in euikaryotic cells where the linear sets of chromosomes are found.
The nucelus is bounded by what?
Two membranes that constitute the nuclear envelope.
Which organelles in eukaryotic cells contain a small amount of their own DNA?
Mitochondrion
Chloroplast
What is the DNA found in other organelles referred to as?
Extranuclear or extrachromosomal DNA
What is the role of mitochondrion?
Functions in ATP synthesis
WHat is the role of the chloroplasts?
In plant and algal cells, which function in photosynthesis.
The DNA found in the organelles such as Mitochondrion and chloroplsats are referred to as what? WHY?
Extranuclear or extrachromosomal DNA To distinguish it from the DNA that is found in the cell nucleus
What is cytogenetics?
The field of genetics that involves the microscopic examination of chromosomes
What is a cytogeneticist?
A scientist who studies chromosomes under the microscope
Observations of eukaryotic species is usually accomplished how? WHy?
Observing the chromosomes as they are found in actively dividing cells.
When the cell is preparing to divide, the chromosomes become more tightly coiled, which shortens them and increases their diameter.
Why does the tightly coiled chromosomes make it easier to observe?
There is a distinctive shape and the number of chromosomes are visible with a light microscope
What are somatic cells?
Any cell of the body except for germ-line cells that give rise to gametes.
What’s a gamete?
A reproductive cell (usually haploid) that can unite with another reproductive cell to create a zygote. Sperm and egg cells are types of gametes.
Gametes are also called what?
Germ cells
How do you think the end results would be affected, in eukaryotic cell observation, if the cells were not treated with a hypotonic solution?
The chromosomes would not be spread out very well and would probably be overlapping. It would be difficult to see individual chromosomes
After the eukaryotic cells have been removed from the body, they are treated with what? Which will do what?
Chemicals that stimulate them to begin cell division and halt cell division during mitosis
The actively dividing cells are subject to what?
Centrifugation to concentrate them
The concentrated preparation during eukaryotic cell division and observation is then mixed with what? which causes what?
Hypotonic solution
It makes the cells swell
The cells swelling causes what, after the addition of hypotonic solution?
The chromosomes to spread out within the cell, making it easier to see individual chromosomes.
What occurs after the chromosomes have spread out during eukaryotic cell observation?
They are treated with a fixative that chemically freezes them so that the chromosomes will no longer move around.
After be treated with a fixative, what happens to the chromosomes?
The cells are treated with a chemical dye that binds to the chromosomes and stains them. They are then placed on a slide and examined.
What is karyotype?
A photographic representation of all the chromosomes within a cell. It reveals how many chromosomes are found within an actively dividing somatic cell.
Most eukaryotic species are what?
Diploid
What is a diploid?
An organism or cell that contains two copies of each type of chromosomes
What is a homolog?
One of the chromosomes in a pair of homologous chromosomes
What are alleles?
An alternative form of a specific gene.
What is locus? (pl. loci)
The physical location of a gene within a chromosome.
How are homologs similar to each other and how are they different?
Similar in size, banding pattern, and carry the same types of genes.
THe alleles of a given gene may be different.
Which of the following is not found in a prokaryotic cell?
a. plasma membrane
b. ribosome
c. cell nucleus
d. cytoplasm
c. cell nucleus
When preparing a karyotype, which of the following steps is conducted?
a. treat the cells with drugs that cause them to begin cell division.
b. treat the cells with a hypotonic solution that causes them to swell.
c. expose the cells to chemical dyes that bind to the chromosomes and stain them.
d. All of the above.
d. All of the above.
How many sets of chromosomes are found in a human somatic cell, and how many chromosomes are within one set?
a. 2 sets, with 23 in each set
b. 23 sets, with 2 in each set.
c. 1 set, with 23 in each set
d. 23 sets, with 1 in each set.
a. 2 sets, with 23 in each set
What is asexual reproduction?
a form of reproduction that does not involve the union of gametes; at the cellular level, a preexisting cell divides to produce two new cells.
The original cell in asexual reproduction is usually called what?
The mother cell.
The two new cells that are produced from the asexual reproduction are called what?
Daughter cells
When species are unicellular, the mother cell is judged to be one _________ and the two daughter cells are what?
One organism
Daughter cells are two separate organisms.
How do bacterial cells proliferate?
Asexual reproduction.
How do unicellular eukaryotes such as amoeba and baker’s yeast reproduce?
Asexually
What is multicellularity?
The property by which an organism consists of more than one cell.
What species have undergone multicellularity?
Plants
Animals
most Fungi
some Protists
Bacteria have what type of chromosomes?
Single Circular chromosomes
How do bacteria divide?
Bacteria duplicate their circular chromosome; they then distribute a copy into each of the two daughter cells, which is called binary fission.
The circular chromsomes in bacteria are in direct contact with what?
The cytoplasm.
Escherichia coli, a common bacterium of the intestine can divide every ________ minutes.
20-30 minutes
Prior to cell division, bacterial cells do what?
Copy, or replicate their chromosomal DNA.
What is binary fission?
The physical process whereby a bacterial cell divides into two daughter cells, during this event, the two daughter cells become divided by the formation of a septum.
What is the function of the FtsZ protein during binary fission?
FtsZ assembles into a ring at the future site of the septum and recruits other proteins to this site that produce a cell wall between the two daughter cells.
FtsZ is evolutionarily related to a eukaryotic protein called what?
Tubulin
Tubulin is the main component of what?
Microtubules, which play a key role in chromosome sorting in eukaryotes.
Both FtsZ and tubulin form structures that provide what?
Cells with organization and play key roles in cell division.
Why is binary fission a asexual form of reproduction?
Because it does not involve genetic contributions from two different gametes.
What is the common outcome of prokaryotic cell division?
To produce two daughter cells that have the same number and types of chromosomes as the original mother cell.
What is the cell cycle?
A series of stages through which a cell progresses in order to divide in eukaryotic cells. The phases are G for growth or gap, S for synthesis ( of the genetic material), and M for mitosis. There are two G phases, G1 and G2.
What is the interphase?
The series of phases G1, S, and G2, during which a eukaryotic cell spends most of its life.
What happens to a cell in the G0 phase?
The cell is temporarily not progressing through the cell cycle or, in the case of terminally differentiated cells, such as most nerve cells in an adulat mammal, will never divide again.
What is the difference between the G0 and G1 phases?
The G1 phase is a phase of the cell cycle when a cell may make the decision to divide. By comparison, the G0 phase is a phase in which a cell is either not progressing through the cell cycle or has made a decision to never divide again.
A cell in the G1 phase may accumulate what?
Molecular changes that cause it to progress through the rest of the cell cycle.
What is a restriction point?
A point in the G1 phase of the cell cycle that causes a cell to progress to cell division.
What happens in the S phase?
The chromosomes are replicated.
What are chromatids?
Following chromosomal replication in eukaryotes, the two copies that remain attached to each other in the form of sister chromatids.
What is the centromere?
A segment of eukaryotic chromosomal DNA that provides an attachment site for the kinetochore.
What are sister chromatids?
Pairs of replicated chromosomes that are attached to each other at the centromere. Sister chromatids are genetically identical.
What’s another name for sister chromatids?
Dyad
What is a monad?
The single chromatid within a dyad
What can also be called a monad?
An unreplicated chromosome
What is the kinetochore?
A group of cellular proteins that attach to the centromere durng meiosis and mitosis.
Kinetochore help to do what?
Hold the sister chromatids together and also play a role in chromosome sorting.
After chromosome replication what happens?
The two copies are called chromatids and are joined to each other at the centromere to form a unit known as a sister chromatids or dyad.
What is the difference between homologs versus the chromatids within a pair of sister chromatids?
Homologs are gentically similar; one is inherited from the mother and the other from the father. By comparison, Chromatids are the product of DNA replication. The chromatids within a pair of sister chromatids are genetically identical.
When the s phase is completed, a cell has twice as many what as what in the G1 phase.
Twice as many chromatids as chromosomes.
The term chromosome is a bit confusing, why?
It originally meant a distinct structure that is observable with the microscope. Therefore, the term Chromosome can refer to either a pair of sister chromatids (a dyad) during G2 and ealy stages of M phase or to a structure that is observed at the end of M phase and during G1.
What structure is observed at the end of M phase and during G1?
A monad.
A monad contains the equivalent of what?
One chromatid.
During the G2 phase, the cell accumulates what?
The material necessary for nuclear and cell division.
What occurs in the m phase?
Mitosis
What is mitosis?
A type of nuclear division into two nuclei, such that each daughter cell receives the same complement of chromosomes.
What is the primary purpose of mitosis?
To distribute the replicated chromosomes, dividing one cell nucleus into two nuclei, so each daughter cell receives the same complement of chromosomes.
When was mitosis first obserbed and by who?
In 1870
By German biologist Walther Flemming.
How was the word mitosis derived?
From the greek word mitos, meaning thread.
Binary fission
a. is a form of asexual reproduction
b. is a way for bacteria to reproduce.
c. begins with a single mother cell and produces two genetically identical daughter cells.
d. all of the above.
d. all of the above.
Which of the following is the correct order of phases of the cell cycle?
a. G1, G2, S, M
b. G1, S, G2, M
c. G1, G2, M, S
d. G1, S, M, G2
b. G1, S, G2, M
What critical event occurs during the S phase of the eukaryotic cell cycle?
a. cells make a decision of whether or not to divide.
b. DNA replication produces pairs of sister chromatids.
c. the chromosomes condense.
d. The single nucleus is divided into two nuclei.
b. DNA replication produces pairs of sister chromatids.
What is the mitotic spindle apparatus?
The structure that organizes and separates the chromosomes during M phase of the eukaryotic cell cycle.
What is another name for mitotic spindle apparatus?
Mitotic spindle
The spindle apparatus is formed from what?
Microtuble-organizing centers (MTOCs)
What are microtuble-organizing centers (MTOCs)?
A site in a cell where microtubles begin to grow.
Microtubles ar produced from what?
The rapid polymerization of tubulin proteins.
In animal cells, the mitotic spindle is fromed from what?
Two MTOCs called Centrosomes.