Chapter 26 Flashcards
Define elution
a
Define mobile phase
a
Define stationary phase
a
Define distribution constant
a
Define retention time
a
Define retention factor
a
Define selectivity factor
a
Define plate height
a
Define longitudinal diffusion
a
Define eddy diffusion
a
Define column resolution
a
Define eluent
a
Describe the general elution problem.
a
What variables are likely to affect the selectivity factor α for a pair of analytes?
a
Name two general methods for improving the resolution of two substances on a chromatographic column.
a
What is gradient elution?
a
26-16. From the data in Problem 26-14. calculate for species Band C
(a) the resolution.
(b) the selectivity factor α.
(c) the length of column necessary to separate the two species with a resolution of 1.5.
(d) the time required to separate the two species on the column in part (c).
