Chapter 22: Cloning and biotechnology Flashcards

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1
Q

What is a clone?

A

A genetically identical copy of an organism or cell.

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2
Q

What is vegetative propogation?

A

The artificial production of natural clones for use in horticulture and agriculture.

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3
Q

What are cuttings?

A

A method of plant cloning that involves planting short sections of stem from a parent plant and providing rooting hormones to encourage growth in genetically identical plants.

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4
Q

What is meristem tissue?

A

Tissue found at regions of growth in plants. Contain stem cells.

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5
Q

What is micropropogation?

A

The process of making very large numbers of genetically identical offspring from a single parent plant using tissue culture.

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6
Q

What is a totipotent?

A

A stem cell that can differentiate into any type of cell and form a whole organism.

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7
Q

What is an explant?

A

A small tissue sample taken from the meristem tissue of a plant for use in micropropagation.

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8
Q

What is a callus?

A

A mass of genetically identical cells.

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9
Q

What are monozygotic twins?

A

Genetically identical twins that have arisen due to the splitting of the early embryo to form two separate embryos.

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10
Q

What is artificial embryo twinning?

A

A method used to clone animals by taking an early embryo and splitting it into multiple smaller embryos before implanting each embryo into a surrogate. Also known as embryo splitting.

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11
Q

What is somatic cell nuclear transfer?

A

A method of producing a clone from an adult animal by transferring the nucleus from an adult cell to an enucleated egg cell and stimulating development. e.g. dolly the sheep.

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12
Q

What is biotechnology?

A

The use of living organisms or parts of living organisms in industrial processes. This could be to produce food, drugs or other products.

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13
Q

What is a culture?

A

A population of one type of microorganism that has been grown under controlled conditions.

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14
Q

What is a closed culture?

A

A culture which has no exchange of nutrients or gases with the external environment.

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15
Q

What is a growth curve?

A

A graph shows the population size of bacteria in a closed culture over time. It is divided into four distinct stages: lag phase, log/exponential phase, stationary phase and death/decline phase.

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16
Q

What is a fermenter?

A

A type of bioreactor used to grow large cultures of microorganisms by providing optimum conditions for their growth and production of product (e.g. insulin)

17
Q

What is bioremediation?

A

The use of microorganisms to break down pollutants and contaminants in soil or water.

18
Q

What is inoculation?

A

The way of introducing the micro-organisms to the growth medium. (e.g. agar or broth)

19
Q

What is aseptic technique?

A

Techniques used to culture microorganisms in sterile conditions so they are not contaminated with unwanted microorganisms.

20
Q

What is serial dilution?

A

A sequence of stepwise dilutions used to reduce the concentration of a solution of suspension.