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MLT 2401 > Chapter 21: Non-fermenting And Miscellaneous Gram Negative Bacilli > Flashcards

Chapter 21: Non-fermenting And Miscellaneous Gram Negative Bacilli Flashcards

1
Q

Non-fermenters

A
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2
Q

List some general characteristics of non-fermenters

A

Do not ferment sugars
Oxidize sugars (saccharolytic)
Gram-negative rods
Mostly oxidase positive
TSI are A/A (non-fermenter)

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3
Q

List some clinical infections associated with non-fermenters

A

Septicemia
Meningitis
Osteomyelitis
Wound infections

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4
Q

What are some risk factors for disease caused by non-fermentative gram-negative bacilli

A

Immunosuppression
Trauma
Foreign body implantation
Infused fluids

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5
Q

Pseudomonas aeruginosa

A
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6
Q

Biochemicals of P. aeruginosa

A

Oxidase positive
Catalase positive
Oxidizes carbohydrates

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7
Q

Characteristics of P. aeruginosa

A

Fluorescent group
Grape like odor
Blue-green pigment

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8
Q

Clinical infections of P. aeruginosa

A

-Wound infections and burn patients
-Pulmonary infections
-Nosocomial UTIs and bacteremia’s
-endocarditis
-Ear infections
-Skin rashes (hot tub syndrome)

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9
Q

Virulence factors of P. aeruginosa

A

Toxins
Proteases
Hemolysin
Slime
Lecithinase
Elastase
Coagulase
DNase

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10
Q

ID characteristics of P. aeruginosa

A

Green sheen on blood agar (Pyocyanin) specific to P. aeruginosa

Arginine dihydrolase (ADH) positive

Grows at 42°C

Citrate positive

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11
Q

T/F are pseudomonas aeruginosa, very drug resistant, and what drug works best

A

True.
Aminoglycosides

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12
Q

P. fluorescens & putida

A
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13
Q

List some features of P. fluorescens and putida

A

Fluorescent group

Low virulence

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14
Q

Infections associated with P. fluorescens and putida

A

UTIs

Post surgical abscess

Septic arthritis

Empyema (the collection of pus in a cavity in the body, especially in the pleural cavity)

Wound infection

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15
Q

Acinetobacter spp.

A
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16
Q

Where can acinetobacter spp. be found?

A

In hospitals, particularly ventilators humidifiers & catheters

17
Q

Clinical infections associated with Acinetobacter spp.

A

UTIs
Pneumonia
Endocarditis
Septicemia
Meningitis

18
Q

Stenotrophomonas maltophilia ID characteristics

A

Catalase positive
Oxidase negative
DNase positive
Esculin positive
Lysine decarboxylase positive
Gelatin hydrolysis

19
Q

Burkholderia cepacia is not a nosocomial pathogen, true or false

A

False

20
Q

Burkholderia cepacia ID characteristics

A

Oxidase negative

Motile

Utilizes glucose maltose, lactose and mannitol

Lysine decarboxylase (LDC) positive

Ornithine decarboxylase (ODC) positive

Arginine dihydrolase (ADH) negative

21
Q

Which of the following is an exotoxin produced by pseudomonas aeruginosa

A

DNase

22
Q

Which pigment produced by pseudomonas aeruginosa is water, soluble, and fluoresces on the short wave UV light

A

Pyoverdin

23
Q

Which of the following sets of reactions distinguishes pseudomonas stutzeri from most other pseudomonas species are

A

Arginine dihydrolase negative and starch hydrolysis positive

24
Q

Which best describes acinetobacter baumannii

A

Saccharolytic
Oxidase negative
Nonmotile

25
Q

Stentrophomonas maltophilia is positive for this test

A

DNase

Other positive tests:
Catalase, Esculin, lysine, decarboxylase

Negative test: oxidase

Gelatin hydrolysis

26
Q

Studies suggest that this plate is most effective in reducing overgrowth, while maintaining good recovery of Burkholderia cepacia

A

B. cepacia-selective agar (BCSA)

27
Q

This test result indicates an isolate is most likely Oligella ureolytica rather than an Alcaligenes spp.

A

Positive phenylalanine deaminase (PDA)

28
Q

Which is the best test differentiate between Brevundimonas vesicularis and B. diminuta

A

Esculin hydrolysis

29
Q

Non-pigmented strains of chromobacterium violaceum have been confused with

A

Aeromonas

30
Q

Which of the following best describes Ralstonia pickettii?

A

Slow grower that grows on MAC agar

MLT 2401 (2 decks)

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