Chapter 21: Fundamentals of Proteomics

Question 1

The identifi cation of proteins by mass spectrometry usually involves protease
cleavage, mostly by ___.

Answer 1

trypsin.

Question 2

One of the most important and often underestimated aspects of a successful proteomics experiment is proper sample handling. ___ ______ is the only biophysical method that consumes the sample. Therefore, contaminations that are introduced to
the method influence not only the outcome of the proteomics experiment, but also the method apparatus itself, and thus subsequent analyses.

Answer 2

Mass spectrometry

Question 3

Contaminations can come from a variety of sources. ___ contamination from flakes of skin and hair can be a major problem, particularly when handling gels and
slices

Answer 3

Keratin