Chapter 20 Review

Question 1

What’s is a plasmid ?

Answer 1

Small circular piece of DNA

Question 2

Where are plasmids found in nature

Answer 2

Bacteria

Question 3

What is the purpose of a plasmid in nature

Answer 3

Protects from antibiotics

Question 4

What are restriction enzymes

Answer 4

Enzymes that cut DNA

Question 5

Where are restriction enzymes found in nature

Answer 5

Bacterial cell

Question 6

What is the purpose of restriction enzymes in nature

Answer 6

Protects bacterial cell

Question 7

How is bacterial DNA protected from restriction enzymes

Answer 7

Methyl group

Question 8

Vitro

Answer 8

Lab

Question 9

Vivo

Answer 9

Body

Question 10

Restriction site

Answer 10

Location that the restriction enzyme recognizes

Question 11

Southern blotting

Answer 11

Pulls band out of gel and puts it on a specific piece of paper

Question 12

Recombinant Dna

Answer 12

Genes from 2 different sources

Question 13

Genetic engineering

Answer 13

Direct manipulation of genes for practical purpose

Question 14

Pcr

And how it’s used today

Answer 14

Polymerase chain reaction

Millions of copies of a target piece of dna is made

Question 15

Restriction fragments

Answer 15

Fragment of dna molecule that has been cleaved by a restriction enzyme

Question 16

Gel electrophoresis

Answer 16

Separates macroMolecules according to certain factors