Chapter 20- Electron Transport Chain Flashcards
What are the 3 features of oxidative phosphorylation?
- Carried out in inner membrane of mitochondria. Reduced versions of NADH + H and FADH2 are produced in the adjacent mitochondrial matrix
- Oxidation of NADH + H produces 2.5 ATP whereas the oxidation of FADH2 produces 1.5 ATP (oxidation and phosphorylation are a couples process)
- The transfer of electrons from NADH + H or FADH2 to O2 goes through a number of carriers (electron transport chain)
Slide 3-4
Review energetics of electron transfer slide 5
Ok
What are the 4 complexes of the electron transport chain?
What proteins make each?
Complex I
Complex II
Complex III
Complex IV
I, II, III are made of Fe-S (iron sulfur) proteins
IV has copper proteins
Protons transferred from inside mitochondria to intermembrane space
Slide 6!!
What are the technical names of each complex in the electron transport chain?
Complex I- NADH dehydrogenase
Complex II- succinate dehydrogenase
Complex III- cytochrome C reductase
Complex UV- cytochrome oxidase
Slide 7
How does the affinity for electrons in NADH change as it goes through the complexes?
Slide 10
Increasing affinity for electrons as this proceeds
Move from low affinity to high affinity
How is free energy relative to O2 affected by reaction process through the electron transport chain?
Released more and more energy as the reaction proceeds
Slide 11
High energy molecule to low energy molecule
What are the two poisons that inhibit the electron transport chain from complex I to complex II (NADH-Q oxidoreductase to Succinate-Q reductase)
Inhibited by the two poisons rotenone and Amytal
What is the one inhibitor that inhibit the electron transport chain from complex III to cytochrome C (Q-cytochrome C oxidoreductase to cytochrome C)
Inhibited by antimycin A
What are the 3 inhibitors that inhibit the electron transport chain from complex IV to oxygen (cytochrome C oxidase to oxygen)?
Inhibited by CN-, N3-, CO
