Chapter 2 - The Invisible World Flashcards
Ziehl-Neelsen staining, a type of ______________ staining, is diagnostic for Mycobacterium tuberculosis.
acid-fast
The ______________ is used to differentiate bacterial cells based on the components of their cell walls.
gram stain
What mordant is used in Gram staining?
crystal violet
Robert Hooke
-first to see cells
-dead cork cells
-compound microscope
Zaccharias Janssen and hans Janssen
invented a lot of microscopes but no evidence
bright field microscope source of energy
light is the source of energy
dark field microscope source of energy
light with opaque lens onto of light source
fluorescence microscope source of energy?
UV light is the source of energy
3 magnifications of 3 objectives of the microscope
10x, 40x, 100x
use of oil immersion lens?
prevents light from refracting in air
total magnification of an object viewed using a 10x ocular lens and a 100x object lens on a brightfieqld microscope
a. 1x
b. 10x
c. 100x
d. 1000x
1000x
4 differences between light and electron microscopes
- source of energy - light = plain white electron = magnets
- electromagnet lens in electron and lens in light in
- medium for light = air medium electron = vacuum
- no occulas in electron
two types of electron microscopes
scanning and transmission
STM
scanning tunnel microscope
ATM
atomic force microscope
