Chapter 2- Cells Flashcards
What do microscopes do?
Microscopes make small objects appear bigger. With a microscope you can zoom in and see the cells that make up living things.
What are animals, plants and other living things made of?
Animals, plants and other living things are made up of tiny cells.
What is the function of a compound light microscope?
These microscopes use light and a number of lenses to form an imageThe specimen (the object being observed) is placed on a rectangular piece of glass called a microscope slide. The specimen needs to be very thin so that light can pass through it. A small, thin piece of glass called a coverslip is placed on top of the specimen. Light is made to pass through the object and the glass lenses, which bend the rays of light. A magnified image of the part of the specimen directly under the objective lens is formed..
What is a microscope called with one eyepiece?
The microscope at right has only one eyepiece; it is a monocular microscope.
What is a microscope called with two eyepieces?
Some school microscopes have two eyepieces; they are called binocular microscopes.
What are microscopes with built-in lights?
These microscopes do not have a mirror at the bottom; they have a light.
What do most microscopes have a selection of?
Most microscopes have a selection of objective lenses, and a revolving nosepiece allows the user to switch from one objective lens to another and thus change the magnification.
How can you calculate the magnification?
You can calculate the magnification by multiplying the eyepiece magnification by the objective lens magnification.
Does changing the objective lens change the size of the image?
It is important to note that changing the objective lens does not change the size of the image; it just shows a tinier part of the specimen at a greater magnification.
What can a typical school microscope magnify up to?
Typical school microscopes can magnify up to 400 times.
What is the microscope measured in?
The measurements of objects viewed under a microscope are usually expressed in microns or micrometres.
1 micron or micrometre (µm) = 0.000 001 m = 0.001 mm
What are the main rules for handling a microscope?
Rules for handling a microscope
- Always use two hands when carrying a microscope: one on the arm of the microscope and the other under the base.
- Place the microscope securely on a flat surface, away from the edge. • Never shine sunlight directly up the microscope tube. You could damage your eyes.
- Use only lens tissues to clean microscope lenses; never use your fingers.
What are the hints for using a microscope?
More hints for using a microscope •
Look down the microscope with one eye, but keep both eyes open — don’t squint.
• Begin focusing a microscope on the lowest magnification.
• Focus a microscope by beginning with the coarse focus. Look from the side and adjust the objective lens so that it is just above the microscope slide.
• Turn the coarse focus knob to move the tube up until the object comes into view.
• Turn the fine focus to make the image of the object as clear as possible.
What is a digital eyepiece?
You can take photos of microscopic objects using a digital eyepiece. This is a special digital camera that works with a microscope.
How can a digital eyepiece be viewed?
Instead of looking through the eyepiece to see the image, the digital eyepiece is connected to a computer and the image can be viewed on a computer screen. The image can be saved, annotated and manipulated in the same way as any digital photo.
What is a stage in a microscope?
Where the slide is placed
What is the function of eyepiece lens?
Used to adjust the amount of light reaching the eyepiece
What is the function of the coarse focus knob?
Makes large adjustments to the distance between the stage and the objective lens; brings the image into focus
What is the function of the fine focus knob?
Makes small adjustments to the distance between the stage and the objective lens; brings the image into focus
What is the function of the stage slide clip?
Holds the slide in place
What is the function of the mirror?
Directs the light towards the specimen
What is the function of the iris adjustment?
Used to adjust the amount of light reaching the eyepiece
What is the function of the slide?
Thin piece of glass where the specimen is placed
What is the function of the revolving nosepiece?
Can be turned to change objective lens
What is the function of the objective lenses?
Magnifies the image
What is a graticule?
This grid is also called a graticule; it is on a glass disc that is often inserted into the eyepiece of a microscope to help measure small objects.
How is the stereomicroscope used?
A stereomicroscope is useful to provide a magnified view of the surface of thicker objects such as rocks, crystals, insects and small flowers.
How much magnification do the stereomicroscopes provide?
These microscopes do not provide as much magnification but they have the advantage that the specimen does not need to be sliced thinly.
How is the hand-held microscope produced?
Like the stereomicroscope, it can be used to view the surface of thick objects, but it plugs into a computer. The image is displayed on the computer screen and can be saved and edited. These devices are cheaper and more portable than stereomicroscopes, but they cannot be used without a computer.
What can the best light microscopes magnify up to?
The best light microscopes can magnify up to 2000 times at the most.
Is the light microscopes sufficient to see viruses
This is sufficient to see bacteria, but viruses are so tiny that they cannot be seen even with the best quality light microscope.
How is the electron microscope used?
An electron microscope is required. These use a beam of particles called electrons, rather than light, to produce an image with up to 2 million times magnification.
How to prepare specimens for view in electron microscopes?
Preparing specimens for viewing is tricky. A specimen needs to be dehydrated and may be coated with a thin layer of metal, so it is not possible to observe living organisms.
What do the images look like when being produced?
The images produced (called electron micrographs) are black and white, although sometimes colours are added later to make particular features stand out or enhance the appearance of the image.
What are the types of electron microscopes?
Scanning electron microscopes (SEM) and transmission electron microscope (TEM).
What does SEM do?
Scanning electron microscopes (SEM) scan the surface of an object and can produce an image of the surface of the object.
What does TEM do?
In a transmission electron microscope (TEM), the electrons pass through a thin section of an object and so the image is a cross-section of the specimen.
What are organelles?
Cells contain ‘little organs’ or organelles. There are different types of organelles and each organelle has a particular function or job. Plant and animal cells appear quite different and contain different organelles.
What is inside an animal cell?
Animal cells are enclosed by a cell membrane that controls the movement of substances in and out of the cell. Inside the cell membrane there is a runny, jelly-like substance called cytosol and a darker area called the nucleus. The nucleus is the control centre of the cell. It contains DNA, a chemical substance that holds the instructions for making particular proteins. These proteins determine what the cell does.
What is mitochondria?
Mitochondria are organelles that can be seen only with a very high-quality light microscope or an electron microscope.
What happens inside mitochondria?
In the mitochondria, glucose reacts with oxygen to release energy. This process is called respiration.
What are plant cells like?
Plant cells tend to be larger and have a more regular shape with some straight edges. This is because they contain a large, water-filled vacuole and are enclosed by a rigid cell wall. The vacuole stores water and minerals.
What are chloroplasts?
Chloroplasts are the oval-shaped organelles found only in plant cells.
What do chloroplasts contain?
Chloroplasts contain a green substance called chlorophyll.
What is the function of chloroplasts?
Chloroplasts use energy from the sun to make food.
Where are chloroplasts found?
Not all plant cells contain chloroplasts. They are found only in leaf and stem cells.
What is the vacuole?
The vacuole is an organelle used to store water and dissolved substances.
What do vacuoles look like?
Vacuoles can look empty, like an air bubble. Plant cells usually have one large vacuole.
What is the function of vacuoles?
The mixture inside a plant’s vacuoles is called cell sap. The red, blue and violet colours that you often see in plant leaves and flowers are due to the substances stored in vacuoles. Most animal cells don’t have vacuoles.
What is the cell wall?
The tough covering around plant cells is the cell wall.
What is the function of the cell wall?
It gives plant cells strength and holds them in shape. Water and dissolved substances can pass through the cell wall.
What are cell walls made of?
Cell walls are made of a substance called cellulose.
What is the cell membrane?
The thin layer that encloses the cytoplasm is the cell membrane.
What is the function of the cell membrane?
It keeps the cell together and gives it its shape. Some substances, such as water and oxygen, can pass through the cell membrane but other substances cannot. The cell membrane controls what enters and leaves the cell.
What is the nucleus?
The nucleus is the control centre of the cell.
What is the function of the nucleus?
It contains DNA in the form of chromosomes and it controls what the cell does and when.
What is the cytosol?
The jelly-like substance inside cells is the cytosol.
What does the cytosol contain?
It contains many important substances, such as glucose, that are needed for chemical reactions that occur inside cells.
What is the cytoplasm?
Cytoplasm = cytosol + all organelles except nucleus