Chapter 19 Quiz

Question 1

Within one cycle of polymerase chain reaction (PCR) the order of the steps is

Answer 1

denaturation, annealing, extension

Question 2

Which of the following primers would have the highest melting temperature (Tm) with its complementary DNA strand
A) 5’-AGCTTGG-3’
B) 5’-AGCTTAAT-3’
C) 5’-AGCTTAA-3’
D) 5’-AGCTTGG-3’

Answer 2

D) 5’-AGCTTGG-3’

Question 3

Genetic analysis techniques usually require many copies of a certain DNA fragment. The two main ways to amplify a DNA fragment are PCR and ____

Answer 3

gene cloning

Question 4

Suppose that you are performing Sanger (dideoxy) sequencing of a sample containing many copies of the DNA fragment below. Which ddNTP tube contains the shortest strand of DNA?
5’ TGGCAACTGAGC-primer annealing site 3’
A) ddGTP
B) ddTTP
C) ddCTP
D) ddATP
E) more than one answer is correct

Answer 4

A) ddGTP

Question 5

True or False? DNA fingerprinting usually involves DNA sequencing of microsatellites, which are short tandem repeat sequences that vary in the number of reagents and are found at multiple loci in the human genome

Answer 5

False

Question 6

When performing gel electrophoresis with DNA large fragments migrate ____ than small fragments mainly because ____

Answer 6

slower; the mobility of the large fragments is hindered more by the agarose gel

Question 7

5’-GCG|C-3’
3’-C|GCG-5’

When DNA is digested by CofI (recognition sequence is shown above) ____ are generated
A) blunt ends
B) overhanging 3’ ends
C) overhanging 5’ ends
D) none of the above

Answer 7

B) overhanging 3’ ends

Question 8

What are the steps of gene cloning in the correct order?

Answer 8

restriction digest, ligation, transformation