Chapter 19 - Molecular Genetics and Biotechnology Flashcards
What are the 4 key innovations in molecular genetics?
Recombinant DNA technology
Polymerase chain reaction
DNA sequencing
Genome editing systems
What are the 6 challenges with working at the molecular level?
Genes are minute
Small physical quantities
Stability of molecules
Complexity of genome (introns, etc.)
Single cell has millions of genes
Nucleotides can’t be seen
What is recombinant DNA technology?
AKA: genetic engineering
Take a segment of DNA and transfer it to another cell to study it
Allows us to locate, isolate, alter, combine, and study DNA fragments/genes
What does recombinant mean?
Taking DNA from two sources and putting them together
What are restriction enzymes and what is their role in recombinant DNA technology?
What are the 3 parts of recombinant DNA technology?
Restriction enzymes - isolate DNA sequence
Visualizing DNA
Cloning vectors - DNA inserted into new source