Chapter 16- DNA Flashcards
Nonpathogenic
Non virulent
Pathogenic
Virulent (lethal/hard to kill by immune response)
Virus (structure)
Protein and DNA
only reproduce
Lytic Cycle
Viral reproduction
Replicates then destroys
Lysogenic cycle
Virus dormant then something causes lytic cycle
Why does immune system want to destroy cell after it is infected with the virus?
So it will not replicate and spread the virus to other cells through mitosis
What do viruses inject?
DNA
Ghost
Virus “shell” after DNA is injected into cell
Watson and Crick
Studied structure of DNA
Phosphodiester
Bond that holds DNA backbone together
Deoxyribose
DNA Sugar
Nucleotide
Phosphate + sugar + nitrogen base
Adenine and thymine bond
Double hydrogen
Cytosine and guanine (bond)
Triple hydrogen
Adenine and thymine (structure)
Purine Double rings (carbon backbone)
Cytosine and thymine (structure)
Pyrimidine Single ring (carbon backbone)
Chargoff’s rule
A=T + C=G =100% Bp
Unit for sizing DNA fragments
Base pairs
DNA charge
NEGATIVE
Why does DNA have a negative charge?
Phosphates in backbone
DNA replication
Copying DNA in the SPhase of interphase
Uncoil, unzip bases, replicate
Single binding protein
Keeps bubbles open in DNA Replication
Topoisomerase
Keeps DNA from tangling
DNA polymerase 3
Builds new DNA strand by attaching nucleotides in 5’ to 3’ direction
DNA Polymerase 1
Builds DNA by taking out RNA primer and fills with DNA
RNA Primase
Lays of down RNA primer and tells DNA pol3 where go start synthesizing DNA
Leading strand
Follows helicase
Lagging strand
Does not follow helicase and creates Okazaki fragments
Okazaki fragments
Fragments on lagging side
DNA Ligase
Seals backbone and puts Okazaki fragments together
What direction does DNA synthesis occur in?
5’ to 3’
Telomere
End of DNA that shortens with age
Telomerase
Add DNA to the end of lagging strand
What kind of cells is telomerase found in?
Cancer and embryonic
Transformation
Process of tranferring an inherited trait by DNA extraction