Chapter 15: Biofilm Flashcards
When does formation of acquired pellicle begin?
Within minutes over all tooth surfaces, calculus deposits and restorations
Significance of acquired pellicle?
Protective
lubrication
nidus for bacteria
attachment for calculus
What are the three basic steps of biofilm formation?
- pellicle formation
2.
Stages of biofilm formation
- formation of pellicle
- Bacterial multiplication
- Matrix formation
- Biofilm growth
- Maturation
Days 1 and 2
gram positive cocci, streptococcus mutans
Days 2 to 4
increase in gram positive cocci
Day 4 to 7
rods and filamentous appears, gram negative appears
Day 7 to 14
vibrios, spirochetes, WBC! Inflammation begins
Day 14 to 21
Gingivitis is evident, clinically
As the number of microorganisms increase, the number of defense cells, leukocytes, ______
increase
What is the difference between supra and sub microorganisms?
Sub gingival includes more anaerobic and motile organisms AND gram- negative
Supra are more aerobic and gram-positive
Composition of biofilm
Organic and inorganic solids- 20%
water- 80%
(Microorganisms makes up 70-80% of the solid matter, which is higher in subgingival)
What are inorganic elements in biofilm?
calcium, phosphorus, magensium, FL
What are organic elements in biofilm?
carbs, protein, and trace amounts of lipids (fats)
Supra gingival biofilm formation begins at the ________
gingival margin at proximal areas
Name Factors influencing biofilm accumulation?
- crowded teeth
- rough surfaces
- occlusion
Detection of biofilm
- direct vision- thin can not see, thicker like a fur coat
- use of explorer or probe- feels slimy coat, soft gooey)
- use of disclosing agent
- clinical record
Why do you disclose after E.O and I.O exams?
It should be done AFTER you’ve thoroughly checked for any suspicious areas or oral lesions.
What bacteria is found majority in healthy gingiva?
majority of aerobic, gram positive
Material alba?
clinical appearance- thick white cottage cheese
effects- gingival inflammation, demineralization
prevention- so thick it can be removed by water spray or irrigator. Biofilm cannot.
