Chapter 13: Chromatography techniques

Question 1

What is a mobile phase?

Answer 1

A solvent which moves over the stationary phase

Question 2

What is the stationary phase?

Answer 2

Components which the mobile phase absorbs to

Question 3

What is paper chromatography?

Answer 3

The stationary phase is paper and has an origin (line with substances to separate). Stationary phase is added to container with mobile phase in it

Question 4

What is the retardastion factor?

Answer 4

Distance solute moves from origin/distance solvent moves from origin

Question 5

What is thin-layer chromtography?

Answer 5

Stationary phase is glass or plastic which is covered in mobile phase. It is more efficient then paper

Question 6

What is column chromatography?

Answer 6

It uses a column with the stationary phase of aluminium oxide or silica (sand like texture, compacted so sample will separate). The mobile phase is added and once soaked the sample is added.

Question 7

What is gas chromatography?

Answer 7

The mobile phase is a gas, the sample is vaporised and combined and carried through a column compacted of the stationary phase. This is all done in an oven

Question 8

High performance liquid chromatography?

Answer 8

Mobile phase is a liquid, sample is injected and they move through a column at a specific flow rate. The column is filled with a solid with a high surface area. As it moves through, it separated and detected by a detector