chapter 12 Flashcards
Why do we need ATP (energy)?
● Anabolic reactions e.g. synthesis of DNA and
proteins
● Active transport
● Movement
● Homeostasis
Why is ATP used for energy?
● ATP is tri-phosphorylated. The removal of each
phosphate releases energy.
● ATP is easily hydrolysed and water-soluble (easy
to transport), making it a useful source of energy.
Name the four main stages of aerobic respiration and state where they occur.
● Glycolysis - cytosol
● Link reaction - mitochondrial matrix
● Krebs cycle - mitochondrial matrix
● Electron transport chain - inner mitochondrial
membrane
Outline the stages of glycolysis.
- Glucose (hexose sugar) phosphorylated to hexose
bisphosphate by 2× ATP - Hexose bisphosphate splits into 2× triose phosphate (TP)
- 2 molecules of TP oxidised to 2× pyruvate
Net gain of 2× reduced NAD (NADH) and 2× ATP per glucose.
Draw a flowchart to explain glycolysis.
https://ibb.co/8PnX31L
How does pyruvate from glycolysis enter the mitochondria?
Via active transport (oxygen is required)
What happens during the link reaction?
- Oxidative decarboxylation and dehydrogenation of
pyruvate to form acetate
Net gain of CO2 and 2× reduced NAD
- Acetate combines with coenzyme A (CoA) to form
acetyl coenzyme A
Draw a flowchart to summarise the link reaction.
https://ibb.co/8MhdZwx
Define “substrate level phosphorylation”.
The synthesis of ATP by the transfer of a
phosphate group from a phosphorylated
intermediate to ADP.
What happens in the Krebs cycle?
Series of redox reactions that produces:
● ATP by substrate-level phosphorylation
● Reduced coenzymes
● CO2 from decarboxylation
Begins when the acetyl group from Acetyl CoA (2C) reacts with oxaloacetate (4C).The cycle regenerates oxaloacetate.
Draw a diagram to explain the Krebs cycle.
https://ibb.co/P66Bfcc
What is the electron transfer chain (ETC)?
● Series of carrier proteins embedded in the
membrane of the cristae of mitochondria
● Produces ATP through oxidative
phosphorylation via chemiosmosis during
aerobic respiration
What happens in the electron transfer chain (ETC)?
● Electrons released from reduced NAD and FAD
undergo successive redox reactions
● The energy released is coupled to maintaining the
proton gradient or is released as heat
● Oxygen acts as the final electron acceptor
How does chemiosmosis produce ATP during aerobic respiration?
Some energy released from the ETC is coupled to the active transport of H+ ions (protons) from the mitochondrial matrix into the intermembrane space.
H+ ions move down their concentration gradient into the mitochondrial matrix via the channel protein ATP synthase.
ATP synthase catalyses ADP + Pi → ATP
Note: chemiosmosis also occurs in photosynthesis in chloroplasts.
Draw a diagram to represent the ETC and chemiosmosis.
https://ibb.co/jM7QYsd