Chapter 10: Lipids Flashcards
1
Q
- What do Storage Lipids contain and what are they built to do?
- What do Structural Lipids contain and what are they built to do?
- What do Other Lipids contain and what are they built to do?
A
- Contain fatty acids and oils. Built to make them stable and easy to disassemble.
- Contain phospholipids and sterols. Built to provide stability and mobility for membrane components.
- Contain cofactors, electron carriers, pigments, hydrophobic anchors, hormones, intracellular messengers. Built for specialized functions.
2
Q
- What makes up the backbone?
- What are fatty acids made of?
- What moleules complex rings?
A
- Sphingosine and Glycerol
- Hydrocarbons
- Cholesterol and derivatives, Cofactors, Signaling molecules, and vitamins.
3
Q
- Which molecules are storage lipids?
- What is the structure of fatty acids? What may they be? What may they contain?
- What do biological fatty acids not contain?
- What are the double bonds usually separated by?
A
- Fatty Acids and Glycerol
- Hydrocarbon Derivatives, Carboxylic Acids with hydrocarbon chains (4-36 carbons). May be fully or partly saturated. Branched or unbranched. May contain three-carbon rings and R-groups: methyl and hydroxyl.
- Do not usually contain conjugated double bonds.
- -CH=CH-CH=CH-
- Usually separated by a methylene group (enzymes recognize this).
- -CH=CH-CH2-CH=CH-

4
Q
- What is the basic setup of Fatty Acid nomenclature?
- How many double bonds do monounsaturated fatty acids contain?
- How many double bonds do polyunsaturated fatty acids contain?
- How many carbons do common fatty acids contain? Do they contain an even number of carbons? How are they build?
A
- Based on chain length : double bond character (Palmitic acid 16:0, Oleic acid 18:1)
- one double bond
- more than one double bond
- 12-24 carbons, unbranched chains
- Even number of carbons
- Acetate condensation (two carbons) = even number.
5
Q
- Are the chains of storage lipids polar or non-polar? Are they water-soluble?
- What are storage lipids physical properties at room temperature?
- What form do saturated chains take?
- What form do unsaturated chains take?
A
- Chain is non-polar.
- Fatty acids tend to be poorly water-soluble.
- Slight solubility in water is due to carboxyl groups.
- At room temperature saturated fatty acids are waxy solids. Unsaturated fatty acids are more liquid, oily
- Saturated chains are linear.
- Unsaturated chains contain bends.

6
Q
- What are Triacylglycerols?
- What do they contain?
- What kinds of bonds do they contain?
- What are their properties?
- What are Triacylglycerols the storage form of?
- What are they cleaved by?
- What are some advantages of using Triacylglycerols as fuels?
A
- Glycerol and Fatty Acids
- Glycerol + 3 fatty acids
- Ester bonds
- Hydrophobic, nonpolar Nearly insoluble in water
- The storage form of both glycerol and fatty acids
- Cleaved by Lipases: hydrolyze ester linkages
- Hydrophobic: No solvation layer for each molecule. One layer around many molecules, saves energy!
- Carbon atoms are highly reduced: Yield more energy than carbohydrates through oxidation (that is, catabolic pathways)
- Example: 15 mg of triacylglycerols = ~1,350 mg of glycogen

7
Q
- What are waxes?
- Are their melting points higher than Triacylglycerols?
- What are they derived from?
A
- Esters of Long chain (14-36 carbon) saturated/unsaturated fatty acids + Long chain (16-30 carbon) alcohols
- Melting points are higher than triacylglycerols
- Waterproofers for feathers
- Prevent evaporation from holly, rhododendron, poison ivy
- Polishes and lotions
- Derived from palm, lanolin (sheep’s wool), beeswax
8
Q
- What make up Glycerophospholipids?
- What make up Spingolipids?
- What make up Sterols?
- What are the above three classes examples of?
- What makes up Phospholipids?
- What makes up Glycolipids?
A
- Glycerophospholipids: Two fatty acids joined to glycerol
- Spingolipids: One fatty acid + one fatty amine (sphingosine)
- Sterols: Rigid core of four fused hydrocarbon rings
- Above three are membrane lipid classes
- Phospholipids: Some glycerophospholipids and sphingolipids with a polar head group + fatty acid
- Glycolipids: Some sphingolipids with a simple sugar instead of phosphate

9
Q
- What is the general structure of Glycerophospholipids?
- What part of the molecule is chiral and which is not?
A
- General Structure Glycerol:
- C1 fatty acid
- C2 fatty acid
- C3 phosphate + polar head group (alcohol)
- Glycerol is not chiral BUT Glycerol + phosphate is chiral

10
Q
- What are Ether Lipids?
- What is their functional significance?
- What my they function in?
A
- Phospholipids with fatty acids in ether linkages. About 50% of heart phospholipids are plasmalogens
- Functional significance of ether, rather than ester, linkage not known.
- MAY function in membrane trafficking, anti-tumor agents
- No strong evidence supports positive roles in health

11
Q
- What is the structure of Sphingolipids?
- What are they derived from?
- What is the structure of Ceramide?
- What are their functions?
A
- 3-carbon backbone: Sphingosine + long chain FA + Polar Head Group. Head group joined by phosphodiester or glycosidic linkage.
- Derived from Sphingosine.
- Ceramide: FA is attached to an amide at C2 = The Parent sphingolipid
- Function:
- Cell recognition molecules, especially in neurons.
- Blood group determinants (A, B, O System)

12
Q
- How do the three classes of Sphingomyelins differ?
- Class 1: Sphingomyelins
- What is their head group?
- What are they similar to?
- Class 2: Glycosphingolipids.
- Where are they contained?
- What do their head groups consist of?
- What are Cerebrosides?
- What are Globosides?
- What separates Blood Group Antigens?
- Class 3: Gangliosides
- What are their head groups?
- What does the carbohydrate portion contain?
A
- Differ in head groups.
- Class 1: Sphingomyelins
- Head group = phosphocholine or phosphoethanolamine.
- Therefore, they are phospholipids similar to phosphatidylcholine: No net charge on the head group at physiological pH.
- Class 2: Glycosphingolipids.
- Mostly on the outer face of the plasma membrane.
- Head groups have one or more sugars on C1 of ceramid.
- Cerebrosides: one sugar on ceramide.
- Globosides: more than one sugar on ceramide. No net charge at pH 7.0 = Neutral Glycolipids
- Class 3. Gangliosides:
- Polar head groups = Oligosaccharides
- Carbohydrate portion has one or more N-acetylneuraminic acids (a sialic acid).
13
Q
- How are Galactolipids different from other molecules?
- Where are they located?
- How numerous are they?
A
- No nitrogen: Different from Glycosphingolipids. No phosphate: Plants conserve phosphate
- Located in the thylakoid membranes of chloroplast.
- The most abundant membrane lipids on earth.

14
Q
- Metabolism of Phospholipids and Sphingolipids: What are signaling products a result of?
- What do membrane lipids become?
- What do Phospholipases cleave?
- What is the product? How is the function of this newly formed molecule?
- How are different signaling molecules made?
A
- Results from a cleavage of certain lipid bonds by specific enzymes.
- Membrane lipids become signaling molecules.
- Cleave phospholipids.
- Product is a Lysophospholipid. Functions in signaling, one secondary product is a fatty acid
- Different signaling products result from cleavage of certain bonds by specific enzymes. Such as Diacylglycerol.
15
Q
- What kind of cells do not synthesized Sterols?
- What is the Sterol nucleus composed of?
- What is it produced from?
A
- Bacterial cells
- Nucleus:
- Three 6-carbon rings.
- One 5-carbon ring.
- Nearly planar
- Produced from acetate: CH3-COO- (Two carbons)
16
Q
- Functions of Sterols: Passive Role: Structural Lipids: What kind of kind molecule is Cholesterol?
- What does Cholesterol alter?
- What does addition of cholesterol do at low temperatures?
- What does addition of cholesterol do at high temperatures?
- Active Roles: What are steroid hormones involved in?
- What do bile acids do?
- What are they precursors for?
A
- Cholesterol is amphipathic, polar head group + nonpolar tail.
- Alters membrane fluidity and assist in lipid transport.
- Prevents hydrocarbon crystalization
- Decreases overall fluidity.
- They are potent signals for gene expression.
- Emulsify fats to make them more accessible for degradation.
- Vitamins and hormones
17
Q
- What are the functions of second messengers?
- What can happen to Phosphatidylinsitol?
- Where do they reside?
- What are their derivitives?
- Where is Phosphatidylinositol 4,5-biophosphate (PIP2) located?
- What are PIP2s fuctions?
- What is PIP2 converted into?
- What is this new molecule cause?
- What is unique about calcium?
- What is Diacylglycerol?
- What does Protein Kinase C do after it is activiated?
A
- A “signal” induces the activation of reactions that produce other molecules and amplify the initial signal.
- The “other molecules” are second messengers.
- The amplification may be in several steps and is part of a Signal Transduction Pathway.
- Can become phosphorylated in several places.
- Phosphatidylinositol resides in the plasma membrane.
- Derivatives are Second Messengers
- A lipid on the inner surface of plasma membrane,
- Functions to…
- Bind cytoskeletal proteins
- Bind specific soluble proteins to promote membrane fusion.
- Bind second messengers after receptor-ligand interactions
- Inositol 1,4,5-trisphosphate (IP3) + diacylglycerol Phospholipase C
- IP3 is a second messenger: Causes release of intracellular calcium.
- Calcium is a second messenger and cofactor for Protein Kinase C.
- Diacylglycerol is a second messenger and cofactor for Protein Kinase C. Protein kinase C becomes active.
- Phosphorylates proteins.

18
Q
- Where do Paracrine Hormones act?
- What are some functions?
- What are they derivitives of?
- What are the three classes?
A
- Act near where they are released
- Diverse Functions: Inflammation (Fever), Pain response (Regulation of blood pressure), Blood clot formation (Gastric acid release).
- Derivatives of Arachidonic acid (20 carbons).
- Prostaglandins, Thromboxanes, and Leukotrienes.
19
Q
- What do Prostaglandins (Eicosanoids) consist up?
- What are the two types of groups?
- How do they regulate cAMP?
- What is cAMP?
- What do Thromboxanes consist up?
- What are they produced by?
- What activities are they involved in?
- How is synthesis inhibited?
- What do Leukotrienes contain?
- What type of struture do they have?
- What are their function?
- What does an overproduction of this molecule cause?
A
- One 5-carbon ring
- Two groups with many subtypes:
- PG E = ether-soluble
- PG F = phosphate (water) soluble
- Regulate cAMP synthesis through Adenylate Cyclase.
- cAMP is a second messenger and a co-factor.
- One 6-carbon ring
- Produced by platelets
- Involved in platelet-derived activities like clotting.
- Synthesis is inhibited by non-steroidal anti-inflammatory drugs.
- Contains 3 conjugated double bonds.
- Open structure
- Serve as signaling molecules.
- Overproduction causes asthma attacks and contributes to anaphyllactic shock.
20
Q
- Where do steroid hormones opperate?
- What are they derivatives of?
- How are they transported?
- Do they have specificity?
- Do they have a high binding affinity?
- What do they induce?
- What are their functions?
A
- Act as messengers between tissues.
- Oxidative derivatives of sterol. Sterol nucleus but no alkyl side chain.
- Move through the blood stream as a complex with proteins.
- Bind to specific receptors.
- Bind with high affinity (Low concentrations have maximal effects)
- Induce gene expression and changes in metabolism.
- Functions: Gender hormones, Anti-inflammatory drugs, Treat asthma and arthritis.

21
Q
- What are vitamines A (Retinol) and D and example of?
- What are the functions of its derivatives? What does Retinoic acid do?
- What is Retinal? What does it produce?
- What can deficiencies lead to?
A
- Example of Lipids that are Hormone Precursors
- Derivatives are metabolic and cell regulators. Retinoic acid regulates epithelial cell gene expression through nuclear receptors
- Retinal: a photosensitive pigment in rod and cone cells.
- Produces a neuronal response
- Precursor is β-carotene.
- Deficiencies lead to skin and eyes disorders (wrinkles and night blindness).
22
Q
- What does separation of lipids utilize?
- During lipid extraction what organic solvent is used for natural lipids, and which are used for membrane lipids?
- What is the method?
A
- Differences in polarity, differences in solubility in non-polar solvents.
- Natural lipids use: Ethyl ether, chloroform or benzene. Membrane lipids use: polar organic solvents (ethanol or methanol).
- Phase extraction using 1(Chloroform):2(methanol):0.8(water). Solvents reduce weak interactions.
- This one phase extracts all lipids.
- Addition of more water and it separates into two phases. Water/methanol phase on top with proteins and sugars (organic phase) on bottom with lipids.
23
Q
- What does absoption chromatography separate lipids based on?
- What is the column packed with?
- Where do polar lipids bind? Where do neutral lipids bind?
- Which lipids will elute first?
- How are left over lipids eluted?
- What are very polar lipids eluted with?
A
- Separation based on polarity.
- Insoluble polar material (silica).
- Polar lipids bind to the column, neutral lipids do not bind to the column.
- Neutral lipids will elute after the first wash with chloroform.
- By using solvents with increased polarity.
- Alcohol
24
Q
- What Gas-Liquid Chromatography used for?
- How are lipids separated?
- How does this method work?
- What does order of elution depend on?
A
- Separates charged lipids best.
- By solubility and volatility in solvent.
- Goes from liquid to gas states with increased temperature. Many lipids are naturally volatile, those that are not have to be derivatized in order to separate them.
- Nature of the solid column support, boiling point of the lipid.