Chapter 1- Methods Flashcards

1
Q

Histology

A

Scientific study of microscopic structures of tissues and organs of the human body.

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2
Q

Another name for Histology

A

Microscopic anatomy

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3
Q

Histopathology

A

The branch of histology that includes the microscopic identification and study of diseased tissue.

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4
Q

What is a pathologist?

A

A medical doctor with specialized training to study medical conditions using human tissue, blood, urine, and other body fluids.

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5
Q

Histotechnology

A

The field of histology that includes the preparation of tissues for microscopic examination.

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6
Q

Telepathology

A

A remote pathology practice that sometimes involves sharing virtual slides online in consultation with other pathology specialists.

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7
Q

The job of a pathology Assistant (PA)

A

A highly trained health professional who works independently under the direction and supervision of a pathologist. They prepare tissue for numerous pathological tests including frozen section, flow cytometry, and immunohistochemical staining.

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8
Q

The job of a Histotechnician (HT)

A

Prepares and processes tissue samples for routine histologic techniques to be examined under a microscope by a pathologist. The histologic techniques include the following: fixation, processing, embedding, microtomy, and staining of human and animal tissue for evaluation.

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9
Q

The job of a Histotechnologist (HTL)

A

Performs both routine and complex histologic techniques such as enzyme histochemistry, immunohistochemistry, in-situ hybridization, and immunofluorescence on tissue samples for microscopic examination by a pathologist to diagnose disease.

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10
Q

The job of a Medical Laboratory Technician (MLT)

A

Collects, processes, and performs routine laboratory tests in blood banking, chemistry, hematology, immunology, microbiology, molecular biology, and/or urinalysis on biological specimens to provide information necessary for the diagnosis and treatment of disease.

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11
Q

The job of a Medical Laboratory Scientist (MLS)

A

Conducts a full range of laboratory tests, from routine to complex, in blood banking, chemistry, hematology, immunology, microbiology, molecular biology, and/or urinalysis on biological specimens to provide information necessary for the diagnosis and treatment of disease. Problem-solving issues with specimens, testing, and instrumentation. Evaluates, develops, and validates diagnostic testing and instrumentation.

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12
Q

The job of a Diener or Autopsy Assistant

A

They are highly trained professionals who assist with autopsies and maintain the morgue of the facility.

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13
Q

Biopsy

A

A procedure to remove a piece of tissue or a sample of cells from the body so that it can be tested in a laboratory.

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14
Q

Purpose of a needle biopsy

A

A medical procedure that removes tissue or fluid from the body with a needle to help diagnose a medical condition.

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15
Q

Purpose of a fine-needle aspiration

A

A long, thin needle is inserted into the suspicious area of the body. It is used to draw out fluid and cells for analysis.

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16
Q

Purpose of a core-needle biopsy

A

A larger needle with a cutting tip used to draw column of tissue out of a suspicious area.

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17
Q

Vacuum-assisted biopsy

A

A suction device that increases the amount of fluid and cells that is extracted through the needle.

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18
Q

Image-guided biopsy

A

Combines an imaging procedure (CT scan, MRI, or ultrasound) with a needle biopsy. Allows the health care provider to access suspicious areas that cannot be felt through the skin (on the liver, lung, or prostate).

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19
Q

Endoscopic biopsy

A

Uses a thin, flexible tube (endoscope) with a light on the end to see structures inside the body. It can be inserted through the mouth, rectum, urinary tract, or small incision in the skin.

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20
Q

Skin biopsy

A

A procedure that removes cells from the surface of the body. It is often used to diagnose skin conditions like cancer.

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21
Q

Shave biopsy

A

A skin biopsy in which the health care provider uses a tool similar to a razor to scrape the surface of the skin.

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22
Q

Punch biopsy

A

A skin biopsy in which a circular tool is used to remove a small section of the skin’s deeper layers.

23
Q

Incisional biopsy

A

A skin biopsy in which the provider uses a scalpel to remove a small area of skin.

24
Q

Excisional biopsy

A

A skin biopsy in which an entire lump or area of skin that appears suspicious is removed.

25
Q

Mohs Micrographic Surgery (MMS) or Mohs Surgery

A

A precise and effective surgical technique using microscopically guided surgical techniques to remove certain types of skin cancers.

26
Q

The most commonly examined/ studied specimens

A

Hematoxylin and Eosin (H&E) stained sections

27
Q

First step of tissue preparation

A

Fixation

28
Q

Purposes of fixation

A
  • Permanently preserves the tissue structure
  • Terminates cell metabolism
  • Prevents enzymatic degradation of cells and tissue by autolysis (self-digestion)
  • Kills pathogenic microorganisms
  • Hardens the tissue by cross-linking or denaturing proteins
29
Q

Most commonly used fixative

A

Formalin; a dilution of formaldehyde.

30
Q

Second step of tissue preparation

A

Preparation of the specimen for embedding in paraffin to permit sectioning.

31
Q

Third step of tissue preparation

A

Staining of the specimen to permit examination by a pathologist.

32
Q

What machine is used to section the specimen during tissue preparation?

A

A microtome.

33
Q

What is acidophilia?

A

The reaction of cationic groups with an acidic dye.

34
Q

What are the substances within the cells and extracellular matrix that will exhibit ACIDOPHILIA?

A
  • Cytoplasmic filaments
  • Intracellular membranous components
  • Extracellular fibers
35
Q

What charge does an acidic dye carry? What is the general formula of an acidic dye?

A

An acidic dye, such as eosin, carries a net negative charge on its colored portion and is described by the general formula [Na+ dye-].

36
Q

What do acidic dyes react to in the cells?

A

Acidic dyes react with cationic components of cells and tissues, the components that carry a positive charge.

37
Q

What is basophilia?

A

The reaction of cationic groups with an basic dye.

38
Q

What are the substances within the cells and extracellular matrix that will display BASOPHILIA?

A
  • Heterochromatin and nucleoli of the nucleus
  • Some cytoplasmic components
  • Extracellular materials with complex carbohydrates
39
Q

What charge does a basic dye carry? What is the general formula of a basic dye?

A

A basic dye carries a net positive charge on its colored portion and is described by the general formula [dye+ Cl-].

40
Q

What do basic dyes react to in the cells?

A

Basic dyes react with anionic components of cells and tissues, the components that carry a negative charge.

41
Q

Immunocytochemistry (IHC)

A

Technique based on the specificity of the reaction between an antigen and an antibody.

42
Q

Expansion microscopy (ExM)

A

A process in which specimens are infiltrated with swellable polymers (hydrogel) to increase their volumes.

43
Q

Steps of expansion microscopy (ExM) (5 steps) (p.11-13)

A

1) Fixation
2) Anchoring
3) Gelation
4) Mechanical homogenization
5) Expansion

44
Q

Provides the illumination for the microscope in the standard optical microscope.

A

The light source

45
Q

Binoculars usually with a magnification of x10 in each ocular lens.

A

Ocular lens or eyepiece

46
Q

The 4 common objective lenses used in Light Microscopy.

A

1) Scanning objective
2) Low power objective
3) High power/ dry lens objective
4) Oil immersion lens objective

47
Q

Gathers the light that passes through the specimens.

A

Objective lenses

48
Q

The flat platform where you place the glass slide to be viewed.

A

Stage

49
Q

Focuses the beam of light at the level of the specimen.

A

Condenser lens

50
Q

Found below the stage. The iris diaphragm and condenser are found there.

A

Substage

51
Q

Regulates the amount of illumination of the slide

A

Iris diaphragm

52
Q

The larger knob located on each side of the microscope toward the base. It moves the sage up and down significantly to bring the specimen into approximate focus.

A

Coarse adjustment knob

53
Q

The smaller knob located with the coarse adjustment knob on the side of the microscope. It is useful at higher magnification providing a very subtle adjustment.

A

Fine adjustment knob