Chapter 1:cell Strructure And Microscopes Flashcards

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1
Q

State 5 differences between eukaryotic and prokaryotic cells

A

-euk, have a nucleus containing their genetic dna, so have membrane bound organelles,
-pro, have genetic dna free floating in cytoplasm bc no nucleus,so no membrane bound organelles

-eukaryotic, have 80s ribosomes, 25nm
-Pro, have 70s ribosomes, 18nm

-euk,have dna that is linear and associated with histones
-pro, have dna that is circular and not associated with histones

-euk are much larger 100 micromètres
Pro are much smaller, 1-5 micromètres

-in euk, plant cell walls made of cellulose and fungi cell walls made of lignin
-in pro, cell walls made of peptidoglycan (polysaccharide and amino acids) and murein

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2
Q

State 3 similarities between eukaryotic and prokaryotic organisms

A

-both have dna
-both have cytoplasm
-both hve cell membranes

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3
Q

Describe the steps involved in the production and secretion of proteins (with the cell structures involved)

A

-translation (protein synthesis) occurs at the ribosomes so a protein is produced
-RER processes proteins made by the ribosomes
-transport vesicles transport these proteins made to the golgi vesicle
-golgi vesicle modifies(eg folding into a polypeptide chain) and packages proteins (eg packaging into secretory vesicles)
-secretory vesicle fuses with cell surface membrane and contents are released through the process of exocytosis

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4
Q

Give the function and describe the structure of the golgi vesicle

A

-function is to modify (eg fold into polypeptide chains) and package proteins and lipids into secretory vesicles
-contents can then be transported to other destinations in the cell eg to produce lysosomes

-fluid filled flattened sacs
-have a layered appearance, so membranes are not continuous and form separate compartments
-are not connected to the nuclear envelope
-bound by a single membrane

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5
Q

Give the function and describe the structure of the rough endoplamsic reticulum

A

Function is to process proteins made by ribosomes

-flattened sacs
-with continuous folds
-connected to the nuclear envelope
-with 80s ribsoomes on the surface

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6
Q

Give the function of lysozomes

A

-specialist forms of vesicles which contain hydrolyctic enzymes which break down waste materials such as warn out organelles in an acidic environment

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7
Q

Function of the nucleus

A

-site of dna replication, so contains the genetic code for each cell
-and site of transcription, so produces mRNA
(Attached to the ER)

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8
Q

What type of membrane is the nuclear membrane in animal cells

A

-a double membrane

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9
Q

Nuclear pore function

A

Allows substances such as ribosomes and mRNA to leave the nucleus
And substances such as enzymes like dna polymerase and signaling molecules to travel in

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10
Q

Nucleolus function and structure

A

-site of ribosome production
-densest region
-contains proteins and rna on it

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11
Q

Chromatin function

A

Materials from which chromosomes are made from ( so a tightly coiled combination of dna and histone proteins)

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12
Q

State the four parts of the nucleus

A

Nuclear envelope- double membrane
Nucleolus
Chromatin
Nuclear pore

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13
Q

Microvilli function in an animal cell

A

Increase the surface area for absorption and diffusion
And so mainly found in the small intestine to maximize nutrient absorption

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14
Q

Cilia function and structure in animal cells

A

-Hair like structures composed of microtubules in a 9+2 formation
-function is to move substances across the surface of a cell

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15
Q

Smooth endoplasmic reticulum function and structure

A

Function, is that it processes and produces lipids, carbohydrates and steroids

-system of membrane bound sacs

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16
Q

Centrosome function and structure

A

-the main microtubule organizing center, involved in cell division when centrioles produce spindle fibres

-consists of two centrioles at right angles t each other and close to the nucleus

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17
Q

Centriole function and structure

A

-produce spindle fibres during mitosis

-one of 2 hollow cylindrical structures made up of microtubules

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18
Q

Microtubules radiating from the centrosome in an animal cell, function and structure

A

-form the cytoskeleton of the cell, so provide support and movement to the cell

-made of of alpha and beta tubulin which form dimers
-dimers form microfilaments
-13 micro filaments form a microtubule

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19
Q

Mitochondria function and structure explanation

A

Function is to carry out aerobic respiration, to produce atp to provide energy

-contain a double membrane
-inner membrane is folded to form projections called Christae, which contain enzymes and give a large surface area
-cristae project inwards into the matrix, which contains enzymes for aerobic respiration
-contain circular dna and 70s ribosomes

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20
Q

Chloroplast function, structure and features explain

A

-function is that they are the site of photosynthesis

-contain many thin thylakoid membranes(folded membranes) which are stacked together to form grana (granular is singular)
-grana linked together by lamella, which are thin and flat thylakoid membranes
(These maximize the surface area being exposed to light)

-contains a starch granule for storage
-contain chlorophyll, which are photosynthetic pigments
-contain circular dna and 70s ribosomes, to synthesize proteins for chloroplast replication and for photysyntheis
-are filled with a fluid called stroma which contains enzymes for photosynthesis

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21
Q

Which organisms are centorosmes found in and which organisms are they not found in

A

-found in animals
-not found in plants, fungi or prokaryotes

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22
Q

Plasmodesmata function

A

Channels that pass through the cell walls of adjoining plant cells, which allow for communication between cells

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23
Q

State the names of the two parts of the vacuole and describe their function.

A

Tonoplast, the partially permeable cell membrane of thr vacuole
Cell sap, consist of minerals, salts, sugars, amino acids waste substances and pigments

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24
Q

State the functions of the vacuole in a plant cell

A

-keep the cell turgid and so provides support
-contains pigment to colour the cell and to therefore attract pollinators
-acts as a temporary food reserve

25
Q

State 3 differences between vacuoles in animal cells and then in plant cells

A

in animal cells,
-small
-many
-not permanent

In plant cells
-large
-one central one
-permanent

26
Q

Function of the cell wall in plant cells

A

-made up of cellulose micro fibrils
-strengthen the cell,
-prevent the cell from bursting from osmosis, so therfore limits cell size

27
Q

State 6 organelles present in both animal and plant cells

A

Ribosome
Smooth ER
Rough ER
-Golgi vesicle and Golgi body
-microtubules
Nucleus, and its 4 major components

28
Q

Give the function and structure of ribosomes

A

-the site if translation, protein synthesis

-composed of equal amounts of rna and proteins

29
Q

Capsule in prokaryotes function and structure

A

-helps cell to retain moisture and adheres to surfaces

-protective slime layer

30
Q

What are mesosomes and what are their function

A

-infoldings of the cell surface membrane in prokaryotes

-they carry enzymes to perform respiration, or photosynthesis or nitrogen fixation

31
Q

Pili function and structure

A

-attach to other bacteria cells, and involved in sexual reproduction

-hair like structures

32
Q

Flagellum function and structure

A

Rotates to move the cell

-a type of microtubule

33
Q

Name 5 structures that are ALWAYS present in prokaryotes, apart form
Capsule
Flagellum
Pili
Mesosomes
Plasmids

A

Cell surface membrane
Ribosomes
Nuceloid, (cirualr dna)
Cytoplasm

34
Q

Name 10 sturtcures in prokaryotes

A

Flagellum
Capsule
Pili
Mesosomes
Plasmids

Always present:
Ribosomes
Cell surface membrane
Cell wall
Nucleoids
Cytoplasm

35
Q

What are viruses

A

-non cellular, infectious particles
-all are parasitic and can only reproduce by infecting living cells (and using ribosomes to produce new viral particles)

36
Q

What is the general size of a virus

A

20-30mm

37
Q

State four structures of a virus particle

A

-nucleic acid core, (genome can be either rna or dna, and single or double stranded)
-capsid, protective protein coat
-lipid envelope, formed from the phospholipid membrane from the cell that they were made in )
-lipid protein

38
Q

Two functions of atp from respiration

A

-used for anabolic reactions, so building large molecules from smaller molecules
-used for active transport, eg moving susbatnces across the cell surface membrane

39
Q

Two uses of atp form respiration in animals

A

-muscle contraction, so coordinating movement within the organism
-cellular processs such as the conduction of nerve impulses

40
Q

What is resolution

A

A measure of how far apart two objects can be whilst still appearing to be 2 separate objects,

41
Q

What is magnification

A

A measure of how many times bigger an observed specimen is, compared to the actual size of

42
Q

Explain how light microscopes work

A

Visible light passes through the specimen, then through the objective lens, then through the eye piece into the eyes

43
Q

Give the max resolution of light microscopes
State what this means
and then explain why they have lower resolutions than electron microscopes

A

-200nm max resolution
-so anything below this will not be seen

-bc visible light has a larger wavelength than electrons
And the larger the wavelength, the more the light will be refracted,
And so more difficult to clearly distuenigh between two points
So therfore less resolution

44
Q

What is resolution dependant upon

A

Upon the different wavelengths of electromagnetic radiations

45
Q

What is the magnification and resolution of a light microscope,
And then magnification and resolution of an electron microscope

A

Light, magnification is x2000
Resolution is 200nm

Electron, magnification is x50,000
Resolution is 0.5nm

46
Q

Explain how a transmission electron microscope works

A

A beam of electrons is passed through the specimen and only those that are transmitted through are seen

47
Q

Explain how a scanning electron microscope is seen

A

A beam of electrons is reflected off of the image so only the surface of it is seen

48
Q

Give two reasons why electrons are useful to use in electron microscopes

A

-because they have very short wave lengths
-and are negatively charged so can be easily focused using electromagnets

49
Q

Explain why electron microscopes must have a vacuum

A

-so that the electrons are not deflected by air particles so that they can therfore travel in a straight line

50
Q

For both light and electron microscopes,
state what the radiation travels through
And the wavelength of this raidtaion

A

Light
-travels through air
Wavelength of 400-700(visible light)

Electron
-travels through a vacuum
Wavelength of 1nm

51
Q

Which type of electron miscrpe has a Lower resolution

A

Scanning electron microscope

52
Q

Give four features of a specimen viewed under a light microscope

A

-has colour
-2D
-taken form above
-specimen can be alive

53
Q

Give 4 features of a specimen observed under a scanning electron microscope

A

-black and white
-3D
-external surface only
-specimen must be dead

54
Q

Give 4 features of a specimen observed under a transmission electron microscope

A

-black and white
-2D
-can see internal structures of organelles
-specimen must be dead

55
Q

In which type of microscopes must the specimen be dead and explain why

A

In transmission and scanning electron microscopes
-bc living specimens have water in the, but for elctrong miscofrpses to work they must occur in a vaccum

56
Q

What measurements are light microsomes taken in and what measurements are transmission microsomes taken in

A

Light= micromètres
Électron= nanomètres

57
Q

In eukaryotic ells, state which parts of the cell can be observed in a light microsphere and which parts can not be

A

Can;
-cell wall
-chorloplats
-nucleus

Can not;
-cell membrane
-mitochondria
-ribosomes
-lyszosomes

58
Q

Explain how to use a light microscope

A

-place slice onto microscope stage
-focus cell using low power objective lens
-use the large knob to make cell come into focus
-use refining knob to sharpen image
-increase magnification using a higher power objective lens

59
Q

Explain how to make a temporary mount

A

-add a drop of water to a glass slide
-obtain a thin section of the tissue and allow it to float
-Stain it
-Cover with a coverslip using a mounted needle