Chapter 1: Amyloids Flashcards
1
Q
Two types of conformational diseases
A
- Loss of function: Misfolded protein mess
- Gain of function: Aggregation
2
Q
Why amyloid structure could not be determined for a lonf time?
A
Amorphous, can’t use X-Ray
Not soluble, NMR is useless
3
Q
General structure of amyloid fibril
A
Beta-sheets parallel to each other and perpendicular to the fibril axis
4
Q
What does birefringence mean
A
Substance has a range of refractive indices
5
Q
What is the barrier for nucleation
A
Insufficient entropy loss compensation by gained molecular forces
6
Q
Nucleation-dependent polymerization
A
Aggregation occurs under critical concentration
At higher concentration, there is a lag before aggregation occurs
Adding a seed instantly starts aggregation
