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Hematology and Immunohematology > Chapter 1,2,3,4 > Flashcards

Chapter 1,2,3,4 Flashcards

1
Q

Which Factor may cause a blood smear to be too thin?

A

the angle of the spreader is low

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2
Q

What is a supravital stain?

A

A method of staining used to examine living cells that have been removed from an organism. IE. New methylene blue

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3
Q

What is an example of a supravital stain?

A

New methylene blue

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4
Q

What is the Normal PH of blood?

A

Between 7.35 and 7.45

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5
Q

The stain most used for a DIFFERENTIAL SMEAR?

A

Wright’s stain

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6
Q

When performing a white blood count, what does the DILUENT do?

A
  1. Hemolyzes the red blood cells
  2. distributes the cells evenly
  3. stains the nucleus of the white blood cells

**note: first 2 are most important

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7
Q

The stain most used for Reticulocytes is?

A

New Methylene blue

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8
Q

Where are the blood antibodies located? Where are the blood Antigens located?

A

Antibodies are located in the plasma, antigens are located on the surface of the RBC.

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9
Q

The movement of leukocytes through the pores of the capillaries is:

A

diapedesis movement

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10
Q

In vivo means

A

in the living body

**remember vivir in spanish means living hence the word vivo

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11
Q

When setting up ESR, it is important to maintain certain standards/environment:

A
  1. ESR tube must be vertical
  2. Kept away from centrifuge
  3. Not kept in an area that is too hot or moist

*NOTE: #1 important

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12
Q

How long is a microhematocrit centrifuged for and at what RPM?

A

5 minutes at 10000RPM

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13
Q

What units is ESR recorded?

A

mm/hr

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14
Q

Any Blood samples must be gently inverted or mixed thoroughly to

A

ensure even distribution of all components

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15
Q

The test that counts immature RBCs?

A

Reticulocyte count

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16
Q

What reagent is used to dilute blood for Hemoglobin estimation?

A

Drabkin’s reagent

Remember: drabkin’s reagent is dangerous because it contains CYANIDE.

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17
Q

What is the black tube(Westergren) used for?

A

ESR

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18
Q

What do we cleanse the area with? before performing a venipuncture or capillary puncture?

A

We use the alcohol swabs which are 70% ALCOHOL

19
Q

When counting WBCs for a differential count, how many cells do we count?

A

100

20
Q

At what angle do we seal a capillary tube with clay for a microhematocrit?

A

90 degrees

21
Q

The immunohematology test that is incubated after collection is

A

cold agglutinins

22
Q

After a blood film(smear) is made

A

we allow it to airdry slowly

23
Q

what angle do we hold the spreader slide when making a blood smear?

A

25 to 30 degrees

24
Q

poikilocytosis

A

abnormally shaped RBCs

25
Q

anisocytosis

A

abnormally sized RBCs

26
Q

crenated cell

A

has scalloped edges

27
Q

normocyte

A

normal sized RBC

28
Q

microcyte

A

abnormally small RBC

29
Q

macrocyte

A

abnormally large RBC

30
Q

antibody

A

fights antigen

31
Q

phagocytosis

A

eats waste such as deteriorated cells or old cells or foreign material

32
Q

What is the difference between serum and plasma

A

They both contain the same analytes but plasma has clotting factors such as fibrinogen and serum doesn’t

33
Q

Where do you read the results of a microhematocrit?

A

Top of the red cell column, do not include buffy coat layer

34
Q

Where are RBCs, WBCs, reticulocytes made?

A

RED BONE MARROW

35
Q

What are the immature white blood cells called?

A

Band cells

36
Q

What are the immature red blood cells called ?

A

reticulocytes

37
Q

Which anemia where the red bone marrow fails to produce RBCs?

A

aplastic anemia

38
Q

Which anemia where the RBCs appear microcytic, hypochromic and some become target cells?

A

thalassemia

*remember: rbcs becoming target cells-think thalassemia

39
Q

Which anemia is caused by abnormal “HGB S”?

A

Sickle Cell

40
Q

The most common type of anemia is

A

Iron deficiency anemia

41
Q

Before reading the hematocrit, we must

A

centrifuge

42
Q

The destruction of red blood cells or red blood cel wall is called

A

hemolysis

43
Q

Thrombocytes are ___________ therefore thrombocytopenia is ______________________.

A

platelets, decrease in platelets

44
Q

Erythroblastosis fetalis

A

a hemolytic anemia in the fetus caused when the mother’s blood type is Rh- and she is pregnant with a Rh+ baby.

Hematology and Immunohematology (1 decks)

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