Chapter 1

Question 1

Hawaiian Punch

Answer 1

Is the extracellular environment and is the same as the insides of
-vesicles
-Golgi
-ER
-Endosome
-Lysosome

Question 2

Mitochondrial Targeting Sequence

Answer 2

Presequence
-rich in basic residues
-no acidic (negative)
-amphipathic alpha-helices
Should be around 50 residues and span both OM and IM

Question 3

Necessary and Sufficient

Answer 3

Deleting of gene results to loss of function and inserting the gene to something that does not naturally have it will result to have that function

Question 4

TOM Complexes

Answer 4

TOM20, TOM22, TOM70

Question 5

TOM20

Answer 5

Is for the pre-sequence of peptide
-has a hydrophobic for amphipathic sequences
Positive side of helix is for specificity

Question 6

Membrane potential in IM of Mito

Answer 6

Membrane potential for inner membrane of mitochondria is necessary to pull pre-sequence into the inner membrane
Without it, the protein would then be clogged into TOM

Question 7

TIM23

Answer 7

Inner membrane translocon that is sealed to prevent protons from leaking

Question 8

Hsp70

Answer 8

Heat-shock proteins
-N-terminal ATPase domain
-C-terminal peptide binding that recognize all unfolded proteins
-a molecular chaperone
-drives translocation with ATP hydrolysis with entropic pulling (horse tail)

Question 9

PAM18

Answer 9

J protein of Hsp70
-recruits and stimulates mtHsp70
-stimulates by ATP hydrolysis

Question 10

Crosslinking

Answer 10

uses
-identify partners of known protein
-protein neighbors
-stable conjugate between 2 proteins

Question 11

Amino Acids for crosslinking

Answer 11

Lysine - alpha-amino group
Cysteine- sulfhydryl (thiol) group

Question 12

Photoactivatable Crosslinkers

Answer 12

UV light generate formation of covalent bonds
Pros: nonselective
Cons: low efficiency and work in dark