Chapter 1 Flashcards
Meaning of CDC
Centers for Disease Control and Prevention
Meaning of OSHA
Occupational Safety and Health
Meaning of CLSI
Clinical and Laboratory Standards Institute
Types of Safety Hazards
Biological
Sharps
Chemical
Radioactive
Electrical
Fire/Explosive
Physical
All health-care facilities have developed
procedures to control and monitor infections occurring within their facilities. This is referred to as
Infection control
It is the place where the infectious agent can live and
possible multiply.
Reservoir
Equipment and other soiled inanimate objects
that can serve as reservoirs
Fomites
the unprotected host touches the patient,
specimen, or a contaminated object (reservoir)
Direct contact
inhalation of dried aerosol particles circulating
on air currents or attached to dust particles
Airborne
the host inhales material from the reservoir (e.g.,
aerosol droplets from a patient or an uncapped centrifuge tube, or when specimens are aliquoted or spilled)
Droplet
ingestion of a contaminated substance (e.g., food,
water, specimen)
Vehicle
from an animal or insect bite
Vector
In the clinical laboratory, the most direct contact with a
source of infection is through contact with
Patient Specimen
When did CDC instituted Universal Precaution
1987
Under this precaution all patients are considered to be possible carriers of blood- borne pathogens. The guideline recommends wearing gloves
when collecting or handling blood and body fluids contaminated with blood and wearing face shields when there is danger of blood splashing on mucous membranes and when disposing of all needles and sharp objects in puncture-resistant containers.
excluded urine and body fluids not visibly
contaminated by blood
Universal Precaution
guidelines are not limited to blood-borne pathogens; they consider all body fluids and moist body substances to be potentially infectious.
personnel should wear gloves at
all times when encountering moist body substances.
A major disadvantage is that they do not recommend handwashing after removing gloves unless visual contamination is present.
Body Substance Isolation
Potentially harmful microorganisms o Frequently present in specimens in the lab
Biologic
shows disease transmission cycle
Chain of Infection
shows disease transmission cycle
Chain of Infection
Chain of Infection happens when there is a continuous link between
Infectious agent ▪ Reservoir ▪ Portal of exit ▪ Means/Mode of transmission Portal of entry ▪ Susceptible host
Percentage of risk of acquiring HBV thru needlestick injury
6-30%
risk of acquiring HIV thru needlestick injury
<1%
risk of acquiring HIV thru direct skin contact
<0.1%
risk of acquiring HIV human bite
0.1-1%
Major disadvantage of BSI
does not recommend handwashing after
removing gloves
when was standard precaution instituted
1996
if hands are VISIBLY SOILED
hand-washing
if hands NOT visibly soiled
hand-rubbing
for suspected Mycobacterium exposure use
N95 respirator
sequence of donning
gown, mask, eye protection, gloves
sequence of doffing
gown and gloves, eye protection, mask
PRIMARY method of infection transmission
Hand Contact
All biologic waste, except urine, must be placed in
appropriate containers labeled with the
biohazard symbol
decontaminated following institutional policy
Incineration
Autoclaving
Pick-up by certified hazard waste company
Urine is discarded by
POURING it into a LABORATORY SINK
disinfection of sink is done daily using
1:5 or 1:10 dilution of sodium hypochlorite (NaClO)
When skin contact occurs:
BEST FIRST AID is
Flush the area with large amounts of
water(running water)
at least how many minutes should area that is affected by chemicals flushed
at least 15 mins
proper way of mixing acid and water
Acid to Water
Hazardous chemicals must be labelled w/ a description of their particular
hazard:
Poisonous Corrosive Flammable Explosive Teratogenic Carcinogenic
National Fire Protection Association (NFPA)
Diamond color for health hazard
Blue
National Fire Protection Association (NFPA)
Diamond color for fire hazard
Red
National Fire Protection Association (NFPA)
Diamond color for reactivity
Yellow
National Fire Protection Association (NFPA)
Diamond color for specific hazard
White
when a fire is discovered what should one do
RACE
Steps in operating a fire extinguisher
PASS
ordinary combustibles: paper, cloth, rubbish, plastic, wood
Type A
Flammable liquids: grease, gasoline, paint, oil
TYPE b
Electrical Equipment and Motor switches
Type C
Flammable metals: mercury, magnesium, sodium, lithium
type d
detonation (arsenal fire)
type e
cooking media: grease, oils fats
type k
goal of total quality management
Improved Patient Outcomes
Overall process of guaranteeing quality patient care
Quality Management (QM)
All of a laboratories processes, procedures, and resources needed to
achieve quality testing
Quality Management System (QMS)
“TAT” or “Turnaround Time” : time required from
Test is requested by a healthcare provide to Result is reported
to the healthcare
provider
Urine should be transported to the laboratory within
2 hours after collection
documentation tool*
→ helps the director to gather info to determine the
root cause analysis and develop a preventive or corrective
action plan
Internal laboratory quality improvement form
Type of water for reagent and control preparation must be specified
distilled water, deionized water, clinical laboratory reagent water
Reagent strips should be checked against known negative and positive
control solutions whether:
- each shift
- once-a-day
- whenever a new bottle is opened
Most frequently encountered equipment or instruments in UA laboratory
refractometer
• osmometer
• automated reagent strip readers
Most frequently encountered equipment or instruments in UA laboratory
refractometer
• osmometer
• automated reagent strip readers
when should professional cleaning of microscope be done
annually
Refractometer:
: calibrated daily or when used against
deionized water SG
1.000
Refractometer:
: calibrated daily or when used against 3% NaCl SG
1.015 ± 0.001
Refractometer:
: calibrated daily or when used against 5% saline
1.022 ± 0.001
Refractometer:
: calibrated daily or when used against 9% sucrose
1.034 ± 0.001
product oriented
all about detection
you try to find defects and correct them while making
used to verify the quality of a product
Quality Control
process oriented
you plan to avoid the defect in the first place
all about prevention
involves processes managing quality
Quality Assurance
closeness of a result to the actual value
closeness of a result to the actual value
closeness of results (of the same specimen) to one another
Precision (of values
ability to produce or obtain the expected result
Accuracy (of a test)
ability to obtain he same results on the same specimen
Precision (of a test)
a substance that has an exact known value that can
produce a solution of an exact concentration
Standards (Calibrators)
to correlate readings of an instrument with those of a standard in order to check the instrument’s accuracy
Calibrate
material or solution with known/established concentrations* that
are similar to the patient samples (viscosity, turbidity, color, composition)
Controls
freeze-dried/dehydrated into powder
lyophilized
most common animal source of control
bovine (cow)
Average of all data points
Mean/Control Mean
Average distance of each data point in a normal distribution from the mean
Standard Deviation (SD)
relative measure of dispersion that is used to determine the variability of
data.
Should be less than 5%
Coefficient of Variation (CV)
gradual change in one direction
increase or decrease over a period of 6 consecutive days
PASS OVER the mean
Trend
rule in trend
7T rule violation
cause of trend
deterioration of reagent
abrupt change
6 or more values on ONE SIDE of the mean, but maintain a constant level
Shift
rule in trend
12x rule violation
cause of shift
deterioration of standard and improper calibration of instrument
Consists of internal monitoring systems (built in to the test system)
INTERNAL QUALITY CONTROL
INTERNAL QUALITY CONTROL can also be called as
“Internal Control” or “Procedural Control”
Use mechanical or electrical device in place of a liquid QC
specimen/control
ELECTRONIC CONTROLS
→Involves testing of unknown specimens received from an outside agency
→Provides unbiased validation of the quality of patient test results
PROFICIENCY TESTING (External Quality Assessment)
Alternative CLIA QC option
→Provides equivalent quality testing for moderate- and high-complexity tests
and provider-performed microscopy procedures (PPMP) tests
→ADVANTAGE: the lab can determine the frequency of QC testing based on
information about the test, the risk assessment, and the accreditation
agencies’ requirements
Individualized Quality Control Plan (IQCP)
For reporting STATtest results and critical values
Telephone (Verbal) Results
Quality management/assessment procedure which compares patient’s test results with the previous ones
DELTA CHECK
Automated comparison of patient results
Autoverification
test results that are significantly lower or higher than the normal reference range
can be LIFE-THREATENING; must be relayed to healthcare provider immediately
Critical Results
lowest level of an analyte that a test can detect
SENSITIVITY
the likelihood of measuring the analyte desired
SPECIFICITY