Chapter 1 Flashcards

1
Q

State the cell theory

A

1- all living organisms are composed of cells
2- cells the the smallest basic unit living structure of life
3- new cells are formed by previous cells diving - omnis cellula e cellula

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2
Q

Schleiden and virchow

A

1 and 2 - scheildon

3- virchow

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3
Q

Describe the properties of cells

A
1 - autonomous 
2- internally regulated
3- organized 
4- self regulating 
5- respond to stimuli
6- capable of movement
7- acquire and use energy 
8- reproduce themselves
9- perform chemical reactions
10- self replication collection of catalysts
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4
Q

Describe cell as a system

A

A cell is a system of connected organelles and structures that work together
It also shows emergent properties

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5
Q

State the central dogma and explain

A

1- info flows from DNA to RNA to Proteins
It means that the genes in DNA provides instructions for making a protein , which is then copies by the RNA , RNA uses the instructions to make proteins. Proteins perform the function and are responsible for the function , form and behaviour of cells and organism

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6
Q

What are the exceptions to the central dogma

A

1- the flow of info is different in the virus . The info moves from DNA to RNA eg in retrovirus due to reverse transcription when virus is inside the host
2- there are more than one type of RNA eg tRNA , rRNA and mRNA however only mRNA is transcribed .
3- there are also non coding RNA types eg microRNA

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7
Q

EVOLUTION TREE :-

What is the ancestral cell

A

Prokaryotic cell

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8
Q

Ancestral cell is divided into

A

Bacteria and archea

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9
Q

Describe bacteria category

A

2 types of bacteria
1- non photosynthetic bacteria
2- photosynthetic bacteria

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10
Q

Describe the category of archea

A

Archea gave rise to SINGLE CELLED EUKARYOTIC CELLS when mitochondria (Bactria) was adapted into the cells
Single cell eukaryotic cells lead to animal, fungi and archea and plants ( this happened after another bacteria ( chloroplast was adapted inside the single celled eukaryotic cells )

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11
Q

Whats the evidence that eukaryotes arose from ancestral archea

A

Due to the structural similarities in eukaryotes, archea and bacteria

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12
Q

Structure =—————

A

Fubction

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13
Q

What does the rule structure = function gives rise to ? Explain

A

Diversity of cells
Because structure = functions , there are many different functions in the cell , organelle or organism therefore each function has a different structure of cells therefore it gives rise to diversity

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14
Q

How do we study cells ?

A

A- we approach cell biology with a REDUCTIONIST VIEWPOINT ( it is based on the premise that studying the part of a whole can explain the character of the entire organism

  • The cells is similar to a working machine with many parts
  • knowing how the ind parts work can explain the whole
  • understanding how cells work can reveal so much about the nature of life
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15
Q

What is system biology

A

Computational and mathematical modelling of complex biological system that will eventually allow for the engineering of biological system

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16
Q

Cell area and volume ?

A

A - 4#r2

V- 4/3#r3

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17
Q

When cells get bigger the SA/V ration———-?

A

Decrease

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18
Q

This causes? Explain

A

Reduction in SA causes
1- decrease in efficiency of movement of molecules
2- decrease in efficiency of absorbing nutrients
3- difficulty in getting rid of wastes
Explanation
When the D of a cell-is 1 um , it takes 100 millisecond to diffuse oxygen , however when the D increases to 10um , it takes 10 million times longer

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19
Q

Cells are usually measured in

A

Um = 10^-6

Nm=10^-9

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20
Q

Whats the mag and res of light microscope

A

Max Mag = 2000x

Res = 100nm

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21
Q

Types of light microscope

A

1- Differential interference contrast microscope
2- Fluorescence microscope
- confocal fluorescence microscope

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22
Q

Describe the fluorescence microscope

A

This uses a fluorescence dye that absorbs certain wavelength and emits a longer wavelength

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23
Q

What is the max magnification and res of electron microscope

A

Mag - 10 million

Res - 1nm

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24
Q

Types of electron microscope and explain

A

Sem - shows the surface of dead cells
Uses metal lining
Tem - shows the cross section of dead cells
Uses metal lining

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25
Q

Purpose of light microscope OVERALL

A
  • The cells can be alive
  • Can view living cells by preparing a slide with water sample
  • reveal SOME CELL COMPONENTS ( the bigger ones)
26
Q

1- Conventional light microscope⁉️⁉️

A

Look at unstained living cells

27
Q

a- Difference interference contrast

A
Three types of optics in this
1- the simplest, brightest- field optics
2- phase contrast optics
3- interference - contrast optics
Used for looking at living cells
28
Q

2- fluorescence microscopy

A

Used to look at location and specific distribution of molecule in the cells
Can also view objects even smaller then 200nm

29
Q

a- Confocal fluoroscence microscopy

A

Generates a sharper image

Constructs a 3D image

30
Q

b- Super resolution fluorescence microscope

A

Position of molecules accurately mapped
Build image with resolution lower at 20nm
Makes 3D image
Allows real time / live cell imaging

31
Q

Electron microscope OVERALL function

A

Look at FINE STRUCTURE OF CELLS

32
Q

a- TEM

A
Same - cross sectional view
See 
DNA translation
Ribosomes
Mitochondria
33
Q

b- SEM

A

3-D image of surface structure

Stomata

34
Q

How is live cell imaging occurs . Explain for auto fluorescence

A

Fluorescence microscope has filter to filter specific wavelength. These wavelength have chromophores which some electrons in biological molecules already have so they can emit light
-Uses visible light ( for observing a molecule that already has chromophores)
- small bandwidth of wavelength illuminate the sample
- some molecules in cell will absorb that wavelength and emit a-longer wavelength (auto fluorescence) because they have chromophores
- because only some wavelength illuminate the sample ,only fluorescently tagged things will show in image
Blue - nucleus
Green - micro tubules
Red - likely auto fluorescence of pigment granules - natural - shown from molecules with natural chromophores

35
Q

Use an example, how can you observe location of protein - JAKE STOUT

A
  • protein doesn’t have chromophores therefore fluorescence dyes or probes are used
  • take an antibody that binds to the specific protein
  • attach a fluorescence molecule
  • Release in cell
36
Q

4 ways to get fluorescence and their specific purpose

A

1- antibody tagged with fluorescence molecule( one of the 2 below- DAPI AND GFP)- to find the location of specific protein, because this binds to it

2- DAPI - a dye that binds to DNA and fluorescence blue
purpose- localization , absence/presence of proteins

3 GFP - green fluorescence from jellyfish
Purpose- localization, distribution and properties of proteins.

4- auto fluorescence of compound already in the cell eg. pollen grain - natural emission of light from biological molecules after they have absorbed light

37
Q

How is SARs - CoV2 virus imaged

A

Using cryogenic electron microscopy
This is a method for imaging frozen hydrated specimens at cryogenic temperature (-160C)
No need for dyes and fixatives
Gives a molecular resolution

38
Q

Cryogenic microscope purpose

A

Generate 3-D image and v v small samples of viruses

39
Q

Prokaryotes

A

Most abundant

Divided into archea and bacteria

40
Q

Eukaryotes

A

Simple unicellular yeast cells
Protozoans - simple
Human and other organisms like plants- complex

41
Q

Difference in prok and euk

A

Prokaryotes

  • no nucleus
  • no membrane bound organelles
  • small (1-10um)
  • one circular piece of DNA
  • sticky capsule
  • complex cell wall
  • only unicellular
  • Simple
  • binary fission
  • Small ribosomes

Eukaryotes

  • nucleus
  • membranes organelles
  • several DNA packaged into strands called chromatin
  • large 10-100um
  • cilia
  • cytoskeleton
  • uni or multi cells
  • complex
  • Mitosis
  • linear dna
  • cell wall simple when present
  • large ribosomes
42
Q

Similarities in prok and euk

A
  • plasma membrane
  • cytoplasm
  • DNA materials
  • Ribosomes that makeprotein
43
Q

Prokaryotes arose ———-
Eukaryotes arose ———-
Humans and Chimps arose ————

A

3.7 billion
2 billion
7 million

44
Q

Cdll culture

A

Growing of cells on petri dish or in a liquid medium IN LAB

Helps examine the Biology, genetics and functions of different types of cells.

45
Q

What is in vitro Example of in vitro

A

When you grow cells in LAB rather than in natural conditions like in body
Eg cell culture

46
Q

Types of cell culture

A

Normal cells from an organism
- these must be given specific growth factor s
- limited lifespan - divide 40x
Immortal cells
George grey took cancer cells from a tumor in Henrietta lacks
Divide forever due to mutation in dna
Used in vaccines

47
Q

Cells in culture display property of their origins

A

Fibroblasts from human skin – continue to secrete proteins that form the ECM

Human neurons make connections with one another in culture

Epithelial cells from human cervix - form a cell sheet in culture

48
Q

In vitro vs In vivo

A

In vitro is easier. When you give drugs and other treatments to see how cells respond .
In vivo is difficult. When you study cells within the whole tissue or organism

49
Q

Why is model organisms used

A

1- easy to manipulate
2- easy to isolate mutants with non functional genes
3 have genome sequenced
4 short lifecycle
5 easily grown
6 have a common ancestor
7 studying one model allows us to understand structure and function of the rest

50
Q

Common models

A

1- E. coli (a prokaryote) –our first understanding of DNA replication, transcription, translation
2- Saccharomyces cerevisiae (yeast) – simplest eukaryote, many mutants
3- Arabidopsis thaliana – fast growing plant, small genome, many mutants
4- Caenorhabditis elegans – ~ 1000 cells, short life cycle
5- Drosophila melanogaster – 1000s of mutants, well characterized genome (fruit flies)
6- Mus musculus (mice) – 1000s of mutants, easiest mammal for genetics studies

51
Q

Organisms and what models they serve

A

C. elegans– model animal

E. coli – prokaryotic model

Drosophila – insect model

Mice- often used as a mammalian model

Arabidopsis – model plant

Zebra fish- vertebrate development models
Zebra fish are transparent so you can deserve development stages such as the embryon

52
Q

GFP

A

GFP is a reporter gene, we can use the sequence of the GFP protein and insert gene promoter to gene of interest

GFP seq (in red )
When the gene is expressed GFP protein will also be made - GENE EXPRESSION ANALYSIS
53
Q

Size of atoms

A

0.2 nm

54
Q

Size of molecules

A

0.2 - a bit more then 100nm

55
Q

Size of organelles

A

100nm - 10um

56
Q

Size of cells

A

10um - 0.2 mm (200um)

57
Q

How small can Electron microscope see

A

0.2nm and bigger

58
Q

How small can super resolution microscope see

A

20nm or bigger

59
Q

How small can light microscope see

A

200nm and bigger

60
Q

How small can an unaided eye see

A

0.2mm or ( 200um)