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BOOKHISTOFINALS2024 > chap 4 > Flashcards

chap 4 Flashcards

1
Q

steps in tissue processing

A
  1. Fixation
  2. Decalcification (optional)-
  3. Dehydration-
  4. Clearing-
  5. Impregnation (Infiltration)
  6. Embedding
  7. Trimming
  8. Section-Cutting (Microtomy)
  9. Staining
  10. Mounting
  11. Labelin
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2
Q

INCREASING concentration of alcohol; 70-95-100%
1. Fixation
2. Decalcification (optional)-
3. Dehydration-
4. Clearing-
5. Impregnation (Infiltration)
6. Embedding
7. Trimming
8. Section-Cutting (Microtomy)
9. Staining
10. Mounting
11. Labelin

A

Dehydration-

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3
Q

removal of alcohol
** Removal of dehydrating agent from tissues to replaced it with a medium that will dissolve the wax with which the tissues is to be impregnated.**

  1. Fixation
  2. Decalcification (optional)-
  3. Dehydration-
  4. Clearing-
  5. Impregnation (Infiltration)
  6. Embedding
  7. Trimming
  8. Section-Cutting (Microtomy)
  9. Staining
  10. Mounting
  11. Labelin
A

clearing

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4
Q

most common clearing agent

A

xylene

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5
Q

Removal of intercellular & extracellular water from tissues after FIXATION & prior to WAX IMPREGNATION/ INFILTRATION

  1. Fixation
  2. Decalcification (optional)-
  3. Dehydration-
  4. Clearing-
  5. Impregnation (Infiltration)
  6. Embedding
  7. Trimming
  8. Section-Cutting (Microtomy)
  9. Staining
  10. Mounting
  11. Labelin
A

Dehydration

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6
Q

not mandatory procedure; if specimen
contains calcium (i.e. bones, teeth) and is carried out after fixation

  1. Fixation
  2. Decalcification
  3. Dehydration-
  4. Clearing-
  5. Impregnation (Infiltration)
  6. Embedding
  7. Trimming
  8. Section-Cutting (Microtomy)
  9. Staining
  10. Mounting
  11. Labelin
A

Decalcification

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7
Q

** involves removal of clearing agent from tissues so that it will be replaced by a medium that will fill all cavities and tissue spaces.**

  1. Fixation
  2. Decalcification
  3. Dehydration-
  4. Clearing-
  5. Impregnation (Infiltration)
  6. Embedding
  7. Trimming
  8. Section-Cutting (Microtomy)
  9. Staining
  10. Mounting
  11. Labelin
A

Impregnation (Infiltration)

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8
Q

Placing infiltrated tissue in a mold containing the embedding medium which is allowed to solidify

A

EMBEDDING/ CASTING/ BLOCKING

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9
Q

cutting off excess paraffin wax to form a 4 sided prism

A

TRIMMING

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10
Q

TISSUE BLOCK: the product of embedding, to be used in cutting.
And cutting tissues in to thin slices

A

SECTIONING/ MICROTOMY

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11
Q
  • To protect tissue from damage/injury
  • To prevent bleaching of sections
  • For permanent safekeeping and to facilitate easy handling andstorage
    Process of applying a mounting medium between the stained tissue section and the coverslip
A

MOUNTING

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12
Q

ndicating year and specimen number for proper identification

A

LABELING!!

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BOOKHISTOFINALS2024 (5 decks)

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