Chap 14 Flashcards
(30 cards)
where does transcription occur
nucleus
characteristics of mRNA
single stranded
A-> U
contains ribose
information storage molecule
what does mRNA leave through
nuclear pore which goes to the ribosome
In transcription, what does RNA polymerase do
unwinds the 2 strands of DNA
where does initiation start for transcription
promoter
how does transcription end
termination sequence of nucleotides
what is a promoter
special sequence of DNA
RNA binds to the promotor
initiates transcription
Elongation
RNA polymerase unwinds DNA about 10 base pairs at a time
in what direction is the template read for elongation
3’ - 5’
What does RNA transcript do
antiparallel to the DNA template strand
adds nucleotides to the 3’ end
can RNA polymerases proofread
no
Termination
specified by a specific DNA base sequence
Introns
interrupts (does not scramble) DNA sequence that encodes a polypeptide
start codon
AUG - initiation signal or translation
Translation
exons are kept because they’re expressed, introns are spliced out
has a 5’ cap and a 3’ poly-a-tail
what are the three kinds of RNA in protein synthesis
mRNA, tRNA, and rRNA
mRNA
convey genetic info from DNA to ribosome
tRNA
carries amino acids for polypeptide assembly
rRNA
catalyzes peptide bonds and provides structure
RNA polymerases are..
processive (single enzyme template binding results in polymerization of hundreds of RNA bases)
How does RNA polymerase differ from DNA polymerase
RNA poly do not need primers and lack a proofreading function
codon
sequence of three bases- each codon specifies a particular amino acid
stop codons
UAA, UAG, UGA. stop translation and polypeptide released
is the genetic code redundant or ambigous
more than one amino acid, genetic code is redundant