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Microbiology > Ch7: Plating Media. > Flashcards

Ch7: Plating Media. Flashcards

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Biology 101 flashcards
1
Q
  1. Bile esculin agar (BEA).
A

Differential isolation and
presumptive identification of
group D streptococci and enterococci.

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2
Q
  1. Bile esculin azide agar with vancomycin.
A

Selective and differential for cultivation of vancomycin-resistant enterococci from clinical and surveillance specimens.

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3
Q
  1. Blood agar.
A

Cultivation of
nonfastidious microorganisms,
Determination of hemolytic reactions.

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4
Q
  1. Bordet-Gengou agar.
A

Isolation of Bordetella pertussis and Bordetella parapertussis.

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5
Q
  1. Brain heart infusion

agar or broth.

A

Cultivation of

fastidious organisms.

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6
Q 
6. Buffered charcoal-yeast 
extract agar (BCYE).
A

Enrichment for Legionella spp.
Supports the growth of
Francisella and Nocardia spp.

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6
Q
  1. Buffered charcoal-yeast extract (BYCE) agar with antibiotics.
A

Enrichment and selection for Legionella spp.

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7
Q
  1. Burkholderia cepacia selective agar.
A

For recovery of B. Cepacia from cystic fibrosis patients.

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8
Q
  1. Campy-blood agar.
A

Selective for

Campylobacter spp.

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9
Q
  1. Campylobacter thioglycollate broth.
A

Selective holding medium for recovery of Campylobacter spp.

Incubated at 4oC for cold-enrichment.

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10
Q
  1. CDC anaerobe

5% sheep blood agar.

A

Improved growth of obligate, slow-growing anaerobes.

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11
Q
  1. Cefoperazone, vancomycin, amphotericin (CVA) medium.
A

Selective medium for isolation of Campylobacter spp.

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12
Q
  1. Cefsulodin-irgasan-novobiocin (CIN) agar.
A

Selective for Yersinia spp.;

may be useful for isolation of Aeromonas spp.

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13
Q
  1. Chocolate agar.
A

Cultivation of fastidioius microorganisms such as Haemophilus spp., Brucella spp. and pathogenic Neisseria spp.

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14
Q
  1. Chromogenic media.
A

Designed to optimize growth and differentiate a specific type of organism. Chromagars are routinely used in the identification of yeasts, methicillin-resistant Staphylococcus aureus (MRSA), and a variety of other organisms.

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15
Q
  1. Columbia colistin-nalidixic acd (CNA) AGAR.
A

Selective isolation of

gram-positive cocci.

16
Q
  1. Cystine-tellurite blood agar.
A

Isolation of C. diphtheriae.

17
Q
  1. Eosin methylene blue (EMB) agar (Levine).
A

Isolation and differentiation of lactose-fermenting and non-lactose-fermenting enteric bacilli.

18
Q
  1. Gram-negative broth (GN)
A

Selective (enrichment) liquid medium for enteric pathogens.

19
Q
  1. Hektoen enteric (HE) agar.
A

Differential, selective medium for the isolatino and differentiation of Salmonella and Shigella spp. from other gram-negarive enteric bacilli.

20
Q
  1. Loeffler’s medium
A

Isolation and growth of Corynebacterium.

21
Q
  1. MacConkey agar.
A

Isolation and differentiation of lactose fermenting and non-lactose-fermenting enteric bacilli.

22
Q
  1. MacConckey sorbitol agar.
A

For the selection and differentiation of E. coli 0157:H7 in stool specimens.

23
Q
  1. Mannitol salt agar.
A

Selective differentiation of staphylococci.

24
Q
  1. Nyw York City (NYC) agar.
A

Selective for Neisseria gonorrhoeae;

supports the growth of Ureaplasma urealyticum and some Mycoplasma spp.

25
Q
  1. Phenylethyl alcohol (PEA) agar?
A

Selective isolation of aerobic gram-positive cocci and bacilli and anaerobic gram-positive cocci and negative bacilli.

26
Q
  1. Regan Lowe?
A

Enrichment and selective medium for isolation of Bordetella pertussis.

27
Q
  1. Salmonella-Shigella (SS) agar?
A

Selective for Salmonella and some Shigella spp.

28
Q
  1. Schaedler agar?
A

Nonselective medium for recovery of anaerobes and aerobes.

Selective for Campylobacter and Helicobacter spp.

29
Q
  1. Selenite broth?
A

Enrichment of isolation of Salmonella spp.

30
Q
  1. Skirrow agar?
A

Selective for Campylobacter spp.

31
Q
  1. Streptococcal selective agar (SSA)?
A

Selective for Streptococcus pyogenes and Streptococcus agalactiae.

32
Q
  1. Tetrathionate broth?
A

Selective for Salmonella and Shigella spp. except S. typhi.

33
Q
  1. Thayer-Martin agar (modified Thayer Martin)?
A

Selective for N. gonorrhoeae and N. meningitidis.

Supports growth of Francisella and Brucella spp.

34
Q
  1. Thioglycollate broth?
A

Supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms,

35
Q
  1. Thiosulfate citrate-bile salts (TCBS) agar?
A

Selective and differential for Vibrio spp.

36
Q
  1. Todd-Hewitt broth supplemented with antibiotics (LIM)?
A

Selection and enrichment for Streptococcus agalactiae in female genital specimens.

37
Q
  1. Trypticase soy broth (TSB)?
A

Enrichment broth used for subculturing various bacteria from primary agar plates?

38
Q
  1. Xylose lysine desoxycholate (XLD) agar?
A

Isolation and differentiation of Salmonella and Shigella spp. from other gram-negative enteric bacilli.

Microbiology (5 decks)

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