Ch4.Culture Media Flashcards
What is Culture media?
It is a medium containing nutrients, growth factors etc in which microbes can grown in.
What are the components of Culture media?
- Nutrients and energy sources
(H2O, CO2,NO2 sources) - Essential minerals
(Na,K,Cl etc.) - Selective agent
(Antimicrobial agents) - Gelling agent
(Agar,gelatine)
What are the types of culture media based on CONSISTENCY?
a) Liquid/ nutrient broth- (no agar)
b) Semi solid media- (Broth+half qty agar)
c)Solid media- (Agar)
Which media is used for fungi and which one isn’t?
-Liquid media/ nutrient broth used,
Solid media not used
-secondary metabolites can be obtained frm liquid media.
What are the types of media based on CHEMICAL COMPOSITION?
1) Natural/empirical
2)Semi-synthetic
3) Synthetic
Natural/empirical media’s chemical composition is __________.
NOT KNOWN.
Semi-synthetic media’s chemical composition is __________.
PARTIALLY KNOWN.
Synthetic media’s chemical composition is __________.
FULLY KNOWN.
2 egs. Of natural media
Milk, blood
2 egs. Of natural media
2 egs. Of semi synthetic media
Potato dextrose agar, Cornmeal agar
2 egs. Of synthetic media
Richards soln. , Glucose medium
Types of routine lab media
Basal, Enriched, Selective, Indicator, Transport and Storage.(BESTIS)
Uses of basal media and few egs.
Used for bacteria growth - limited nutrients provided.
Egs. Nutrient broth/agar, peptone water.
Description of enriched media and few egs.
Enriched by addition of blood,serum,egg.
Egs. Lowenstein Jensen media, Blood agar media.
Uses of selective media and few egs.
•Favour growth of particular bacteria by inhibiting growth of undesirable bacteria or fungi.
•Antibiotic added.
Egs.
Why is an indicator media used? Few egs
Media used as indicator is added nd growth of particular organism causes colour change.
Eg. Mc Leod’s media, Wilson-Blair media
Why is a transport media used? Few egs.
Used when specimen can’t be cultured soon after collection and used to transport the sample.
Eg. Cary-Blair media, Amieo media, Stuart media
Why is an storage media used? Few egs
Used to store microbes for a long period of time.
Eg. Egg saline media, Robertson’s cooked meat broth
