ch15: sequencing

Question 1

DNA sequencing

Answer 1

determination of actual base sequence of DNA

Question 2

dideoxynucleotides (ddNTPs) are used as

Answer 2

chain terminators in DNA synthesis reactions; cannot code for anymore bases once attached

Question 3

gel electrophoresis

Answer 3

separates DNA strands by size

Question 4

genes are identified by

Answer 4

open reading frames

Question 5

sequence annotation

Answer 5

the addition of information, such as ORFs, to the basic sequence information

Question 6

plasmids

Answer 6

small circular chromosomes that replicate themselves because they have an origin of replication

Question 7

three parts of a plasmid

Answer 7

1) origin or replication
2) selectable makrer
3) multiple cloning site

Question 8

SNPs (single nucleotide polymorphisms)

Answer 8

single nucleotide base substitutions that occur in more than one percent of the general population; about one in every 300 nucleotide base pairs

Question 9

haplotype

Answer 9

combination of SNPs used to identify groups of individuals with similar DNA