Ch 9 Repair Enzymes Flashcards
MutL
Activates MutH in E Coli
MutS
E Coli-scans DNA
MutH
Cleaves DNA in E Coli
MSH
Human-MutS
Interacts - sliding clamp
MLH/PMS
Homologue of MutL
Photolyase
Photoreactivation for Direct Reversal
E Coli
Methyltransferase
Group removal in Direct Reversal
Glycolase
MutM in E Coli
Removes G in oxoG:A
Base Excision Repair
MutT
Error Avoidance-E Coli
Remove G in oxoG b4 incorporation
MutY
E. Coli- Failsafe in Base Excision
Remove A in oxoG:A
MTH1
=MutT
In human Chr7p22
UvrA
Complex + Uvrb
Scan DNA in E. Coli Nucleotide Excision Repair
Uvrb
Recruits Uvrc
NER E.Coli
UvrC
Cut 8nt on 5’
4-5 on 3’
NER E.Coli
UvrD
Helicase
NER and Mismatch Repair in E. Coli
XPC
Human NER
homo 4 UvrA
XPA-XPD
Human NER
homo 4 UvrB
ERCC1-XPF
Cut 5’ end in human NER
homo 4 UvrC
XPG
Cut 3’ end in human NER
Homo 4 UvrC
TFIIH
Transcription Coupled Repair
NER
LexA
Transcriptional Repressor
E. Coli Translesion Synthesis
UmuC/UmuD
DNA Poly V
E. Coli Translesion Syn
DinB
DNA Poly IV
E. Coli Translesion Syn
Ku 70/Ku 80
NHEJ
VDJ Recombination
DNA-PKcs
Eu- NHEJ & VDJ Recomb
Artemis
Eu- NHEJ & VDJ Recomb
BRCA1
Tumor Suppress Gene
Eu- Homo Recombination
Breast Cancer 1
BRAC2
Tumor Suppressor
Bind & regulate proteins made by Rad51
Eu- HRR
Rad51
Homo 4 Pro RecA & yeast Rad51
Eu-HRR
Chk1
Prevent entry to mitosis
UV damage, replication error, p53 activation
Chk2
Prevent Mitosis Entry
Interact BRCA1 = BRCA1 restore survival after damage
Repair of ionizing radiation
RAG1 & RAG2
Eu- VDJ recomb
p53
47 kDa Transcription Factor-recognize DNA erro -DNA Damage, osmotic shock, nucleotide depletion, oncogene start Hold cell G1 or S, or apoptosis 50% cancer has mutated p53
Cdk2
Cell cycle regulator
ATM
Target- p53, BRCA2, chk2, H2AX, RAD,
Activates- NHEJ, HRR, p53/chk2
Ds DNA Breaks