Ch. 9 Flashcards
Where does glycolysis occur?
Cytoplasm
What does glycolysis accomplish for the cell?
Generates ATP and pyruvate
What is the overall net reaction of glycolysis?
Generates 2 pyruvate and 2 ATP
What are the key enzymes in glycolysis?
- Hexokinase (glucose to glucose-6-P)
- Phosphofructokinase-1 (fructose-6-P to fructose-1,6-BP)
- Pyruvate kinase (phosphoenolpyruvate and ADP to pyruvate and ATP)
What is metabolism?
The collective biochemical reactions in a living organism that converts chemical energy into work.
- Catabolic and anabolic pathways
What are aldoses?
Aldehyde containing sugars (11)
- Glucose
What are ketoses?
Ketone containing sugars(11)
- Fructose
What relation do D and L sugars have to each other?
They are enantiomers
- D is -OH on right and L is -OH on left
What is step 1 of glycolysis?
Hexokinase is used to phosphorylate (ATP -> ADP) Glucose which becomes Glucose-6-P
What is step 2 of glycolysis?
Phosphoglucoisomerase is used to transform Glucose-6-P to Fructose-6-P. The six member ring changes to a five member ring (8)
What is step 3 of glycolysis?
Phosphofructokinase-1 is used to phosphorylate (ATP -> ADP) Fructose-6-P to Fructose-1,6-BP (9)
What is step 4 of glycolysis?
Aldolase is used to cleave Hexose into two trioses. The reaction uses Schiff base as an intermediate (10)
What is step 5 of glycolysis?
Triose phosphate isomerase makes Dihydroxyacetone-P into an enediol intermediate which can be turned to Glyceraldehyde-3-P (14)
What is step 6 of glycolysis?
Glyceraldehyde-3-phosphate dehydrogenase is used to generate NADH from NAD+ and Pi and turns Glyceraldehyde-3-p to 1,3 bisphosphoglycerate (15)
What is step 7 of glycolysis?
Phosphoglycerate kinase is used to phosphorylate ADP -> ATP and turns 1,3 Bisphosphoglycerate into 3-phosphoglycerate