Ch. 9 Flashcards

1
Q

Where does glycolysis occur?

A

Cytoplasm

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2
Q

What does glycolysis accomplish for the cell?

A

Generates ATP and pyruvate

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3
Q

What is the overall net reaction of glycolysis?

A

Generates 2 pyruvate and 2 ATP

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4
Q

What are the key enzymes in glycolysis?

A
  • Hexokinase (glucose to glucose-6-P)
  • Phosphofructokinase-1 (fructose-6-P to fructose-1,6-BP)
  • Pyruvate kinase (phosphoenolpyruvate and ADP to pyruvate and ATP)
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5
Q

What is metabolism?

A

The collective biochemical reactions in a living organism that converts chemical energy into work.
- Catabolic and anabolic pathways

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6
Q

What are aldoses?

A

Aldehyde containing sugars (11)
- Glucose

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7
Q

What are ketoses?

A

Ketone containing sugars(11)
- Fructose

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8
Q

What relation do D and L sugars have to each other?

A

They are enantiomers
- D is -OH on right and L is -OH on left

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9
Q

What is step 1 of glycolysis?

A

Hexokinase is used to phosphorylate (ATP -> ADP) Glucose which becomes Glucose-6-P

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10
Q

What is step 2 of glycolysis?

A

Phosphoglucoisomerase is used to transform Glucose-6-P to Fructose-6-P. The six member ring changes to a five member ring (8)

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11
Q

What is step 3 of glycolysis?

A

Phosphofructokinase-1 is used to phosphorylate (ATP -> ADP) Fructose-6-P to Fructose-1,6-BP (9)

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12
Q

What is step 4 of glycolysis?

A

Aldolase is used to cleave Hexose into two trioses. The reaction uses Schiff base as an intermediate (10)

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13
Q

What is step 5 of glycolysis?

A

Triose phosphate isomerase makes Dihydroxyacetone-P into an enediol intermediate which can be turned to Glyceraldehyde-3-P (14)

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14
Q

What is step 6 of glycolysis?

A

Glyceraldehyde-3-phosphate dehydrogenase is used to generate NADH from NAD+ and Pi and turns Glyceraldehyde-3-p to 1,3 bisphosphoglycerate (15)

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15
Q

What is step 7 of glycolysis?

A

Phosphoglycerate kinase is used to phosphorylate ADP -> ATP and turns 1,3 Bisphosphoglycerate into 3-phosphoglycerate

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16
Q

What is step 8 in glycolysis?

A

Phosphoglycerate mutase causes a phosphoryl shift causing 3-Phosphoglycerate to become 2-Phosphoglycerate

17
Q

What is step 9 in glycolysis?

A

Enolase dehydrates 2-Phosphoglycerate causing it to become Phosphoenolpyruvate

18
Q

What is step 10 in glycolysis?

A

Pyruvate kinase turns Phosphoenolpyruvate into Pyruvate by phosphorylating ADP and turning it to ATP

19
Q

What happens to pyruvate after it is synthesized?

A

Aerobic conditions:
- ATP produced via citrate cycle and ETC

Anaerobic conditions:
- Production of lactate
- Ethanol production