CH 6 Flashcards

1
Q

Cell Division

A
  • when unicellular organisms (bacteria) divide they are reproducing
  • when multicellular cells divide they are replacing dead or damaged cells or growing.
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2
Q

Prokariotic Reproduction

Asexual Reproduction

A

-99% of time in unicellular organisms
- produces offspring
-genetically identical to parent cell
-no partner involved
- no exchange of genetic material between
cells

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3
Q

Common forms of Asexual reproduction

A
  • In unicellular organisms, Budding and

Binary fussion

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4
Q

Budding

A

(Typically seen in yeast)
- New smaller offspring (clone) “buds” from
the original cell (parent)
- breaks away from parent cell, then grows
to the mature size

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5
Q

Binary Fission

A

-Original parent cell grows in size, including
replication of cellular content.
- cell splits into TWO identical cells, typically
seen in bacteria

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6
Q

Conjugation

.1% chance

A
  • sexual reproduction in microbes
  • rare, 2 bacteria reproduce sexually
  • one cell makes copy of its chromosome or plasmid and transfers the copied DNA
  • exchange of genes
  • Resulting in offspring or genetically different cell from its parent
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7
Q

Life Cycle of Prokaryote

4 Microbial Growth Phases

A
  • bacteria introduced to new mediums ideal for growth, they go through 4 phase
  • Lag, Log, stationary, death or decline
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8
Q

Lag Phase

A

-Bacteria adapting to new conditions, no reproduction, little metabolic activity

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9
Q

Log Phase

A
  • Peak reproductivity and metabolic

activities

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10
Q

Stationary Phase

A
  • # of new cells are produced offset by # ofcells dying, nutients begin to decline and
    waste begins to accumulate
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11
Q

Death Phase

A
  • Conditions inhospitable,nutrients deplete,
    waste inhibits growth/reproduction. Cells
    die, population declines
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12
Q

Physical Influences on Growth

A

Most bacteria grow best under moderate condition like

     - Ph near 7
     - low to no salt
     - temperature near 37C
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13
Q

Growth preferences

PH

A
  • Acidophile

- Alkaliniphile/Alkalophile

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14
Q

Growth Preferences

Salt

A
  • Halophile

- Most fermented food

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15
Q

Growth Preference

Temp.

A
  • Psychrophile
  • Mesophile
  • Thermophile
  • Extreme thermophile
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16
Q

Oxygen and Growth

A
  • Obligate Aerobes
  • Microaerophiles
  • Obligate Anaerobes
  • Facultative Anearobes
17
Q

Obligate Aerobes

A

Requires oxygen for growth

18
Q

Microaerophiles

A

Grows best when O2 is less than normal

atmospheric concentration of 21%

19
Q

Obligate Anaerobes

A

Does not grow if Oxygen is present. Grows in abscense of oxygen

20
Q

Facultative Anaerobes

A

Grows in presence of oxygen or without

21
Q

Testing for Oxygen

“Gas Pak”

A

Chemically remove oxygen in sealed chamber from petri plate

22
Q

Testing for Oxygen

“FTM”

A
  • Fluid Thioglyate medium
  • top layer oxygen, no oxygen on bottom layer
  • innoculate by stabbing media
  • incubate 24 hr.
23
Q

Reduce leves of oxygen to less than 21% by burning a candle in sealed jar. Combistion removes some oxygen, but not most.

A

Testing for Microaerophiles

“Candle Jar”

24
Q

Some bacteria produce slime which they use to attach to substrate/surface. Forming a community called. Plaque is a type of ________, that has harderdened onto teeth enamel.

A

Biofilms (bacterial interactions)

25
Q

A phenomenon whereby microbes communicate w/one another to “sense” their population # and other key info.

A

Quorum Sensing

26
Q

The process whereby vegetative cells turn into spores. usually triggered by lack of key nutrients.

A

Sporulation (spore formation)

27
Q

A medium, agent or tool used to cultivate/grow microorganisms.

A

Media

28
Q

A media whereby you know exactly everything about the media. Non-biological ingredients (ex. Salt, sugar)

A

Defined Media

29
Q

Exact ingredients and their amounts is UNKNOWN. Ingredients are biological in origin. (Milk, blood)

A

Complex Media

30
Q

Promotes the growth of only a certain type of bacteria while inhibits others.

A

Selective Media

31
Q

Allows for differentiation of one species of bacteria from another based on growth/appearence. (EMB. E Coli, metallic green sheen like beetles shell)

A

Differential Media