Ch 15 Peripheral Blood Film Flashcards
What are the components of a peripheral smear
WBC estimate
platelet estimate
relative proportion of leukocyte type
cell morphologic structure
What is platelet satellitosis
platelets stick to WBCs can cause pseudothromobytopenia in count. because of EDTA, causes false increased WBC count and falsely low platelets
What color might specimen turn under the microscope if the slide is too old, what if its not too old?
too blue
not old: when increased proteins are present also too blood
How many cells are counted in a WBC differential
100
What are the WBC abnormalities
toxic granules, dohle bodies, reactive lumps, auer rods
What should you assess in RBC morphology
size, variation in size, color, shape, inclusions
How many RBCs should be present per field in a platelet estimate
200
What WBC parameters should you report
Total WBC count, differential %, and morphology
What platelet parameters should you report
platelet count, MPV, morphology
What is it called if WBC count is elevated? decreased?
elevated: leukocytosis
decreased: leukopenia
What values are included in WBC differential
relative counts, absolute counts
What is a left shift
neutrophilic cells bands and younger in peripheral blood
What is it called when WBC and Hb are all decreased or increased
decreased: pancytopenia, indicates leukemia or aplastic anemia
increased: pancytosis, indicates polycythemia vera
Normal MPV range
6.8-10.2
What does an increased MPV indicate?
large platelets
What is the normal platelet count
150-450
what are the staining characteristics of a smear
acidic components: stains acidic and basophilic components like RNA
basic components: stains basic, and eosinophilic components like Hb and granules
artifacts
What is the calculation of an absolute WBC count
(WBC count)(% of each type of WBC)= absolute value
add all together = total WBC count
What are the RBC parameters in CBC
total count hb hematocrit MCV MCH MCHC RDW morphology
What is the term for when there are varying sizes of RBCs, what parameter can reveal it
RDW elevated anisocytosis
How do you calculate the adjusted platelet count
platelet count x 1.1
formula for MCV
hct/RBC x10
formula for MCH
hb/RBC x10
formula for MCHC
hb/hctx100
ranges for MCV
80-100
ranges for MCHC
32-36
