Ch 15 Peripheral Blood Film Flashcards

1
Q

What are the components of a peripheral smear

A

WBC estimate
platelet estimate
relative proportion of leukocyte type
cell morphologic structure

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2
Q

What is platelet satellitosis

A

platelets stick to WBCs can cause pseudothromobytopenia in count. because of EDTA, causes false increased WBC count and falsely low platelets

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3
Q

What color might specimen turn under the microscope if the slide is too old, what if its not too old?

A

too blue

not old: when increased proteins are present also too blood

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4
Q

How many cells are counted in a WBC differential

A

100

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5
Q

What are the WBC abnormalities

A

toxic granules, dohle bodies, reactive lumps, auer rods

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6
Q

What should you assess in RBC morphology

A

size, variation in size, color, shape, inclusions

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7
Q

How many RBCs should be present per field in a platelet estimate

A

200

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8
Q

What WBC parameters should you report

A

Total WBC count, differential %, and morphology

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9
Q

What platelet parameters should you report

A

platelet count, MPV, morphology

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10
Q

What is it called if WBC count is elevated? decreased?

A

elevated: leukocytosis
decreased: leukopenia

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11
Q

What values are included in WBC differential

A

relative counts, absolute counts

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12
Q

What is a left shift

A

neutrophilic cells bands and younger in peripheral blood

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13
Q

What is it called when WBC and Hb are all decreased or increased

A

decreased: pancytopenia, indicates leukemia or aplastic anemia
increased: pancytosis, indicates polycythemia vera

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14
Q

Normal MPV range

A

6.8-10.2

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15
Q

What does an increased MPV indicate?

A

large platelets

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16
Q

What is the normal platelet count

A

150-450

17
Q

what are the staining characteristics of a smear

A

acidic components: stains acidic and basophilic components like RNA
basic components: stains basic, and eosinophilic components like Hb and granules
artifacts

18
Q

What is the calculation of an absolute WBC count

A

(WBC count)(% of each type of WBC)= absolute value

add all together = total WBC count

19
Q

What are the RBC parameters in CBC

A
total count
hb
hematocrit
MCV
MCH
MCHC
RDW
morphology
20
Q

What is the term for when there are varying sizes of RBCs, what parameter can reveal it

A

RDW elevated anisocytosis

21
Q

How do you calculate the adjusted platelet count

A

platelet count x 1.1

22
Q

formula for MCV

A

hct/RBC x10

23
Q

formula for MCH

A

hb/RBC x10

24
Q

formula for MCHC

A

hb/hctx100

25
Q

ranges for MCV

A

80-100

26
Q

ranges for MCHC

A

32-36