Ch 14: ERG Conversion in Mito and Chloro Flashcards
The Four Compartments of Mitochondria and Roles:
Matrix:
Intermembrane Space:
Inner Membrane: E-Transport Chain, H+ Gradient, ATPase.. Folded into”cristae”
Outer Membrane: Super high in “porin proteins” - acts like a sieve.
Where are Mitochondria located?
Mitochondria are located near sites of high ATP utilization.
Mitochondria ATP synthesize overview: steps
Pyruvate and FA enter from cytosol
pyruvate and FA combine to form acetyl CoA
Acetyl CoA enters CA cycle - CO2 and NADH
NADH donates electron to E-transport chain and becomes NAD+
E-transport chain pumps out H+
ATP synthase utilizes H+ gradient to ADP + Pi –> ATP
Redox Potential: ??
Strong Reducing Agent:
Strong Oxidizing Agent:
Redox Pot = Measure of Tendency of Mol couple to gain e-
St Red Ag = e- donor = (-) delta E
St Ox Ag = e- acceptor = (+) d E
Three Proteins that make up the Electron Transport Chain
NADH dehydrogenase complex
Cytochrome b-c1 complex
Cytochrome oxidase complex
ETC is a series of ____ that transfer _____ from a more ____ to a more _____, while pumping ____ out of the _____ into the _____.
Electron Chain Molecules
Electrons
Negative redox potential
Positive redox potential
protons
mitoplasm
perimitochondrial space
Redox potential (increases/decreases) along the ETC
.
Name the compartments of the chloroplasts
Stroma
Granum
Thylakoid
What are the two types of photosynthesis reactions and where do they take place?
1) Light-dependent reactions (Occur on the thylakoid membranes)
2) Light-independent reactions (occur in the stroma)
Two Types of Photosynthesis
What do they use? What do they make? What do they do?
Light-dependent uses NADP and ADP+Pi to produce NADPH and ATP (uses light to cleave H2O into O2)
Light-independent rxns use NADPH and ATP to produce NADP and ADP+Pi (CO2 –> 3 carbon compound –> glucose/AA/FA)
Glycolysis Net RXN
C6H12O6 + 2ADP + 2Pi + 2NAD+ –> 2Pyruvate + 2ATP + 2NADH + 2H+ 2H2O
Pyruvate Decarboxylation
Pyruvate converted into acetyl CoA in the mitochondria matrix
Citric Acid Cycle
Acetyl CoA –> NADH + FADH2 + CO2
Oxidative Phosphorylation
Electrochemical gradient caused by NADH and FADH2 oxidation provides energy for ATP synthase to phosphorylate ADP into ATP.
How do the two photosystems work together and where are they located?
(Light Dependent RXNs)
The photosystems are located in the thylakoid membranes.
photosystem II absorbs a photon splitting water into O2 and passes electrons through acceptors that generate ATP
photosystem I receives electrons from photosystem II… Passes electrons through acceptors: NADH+ –> NADPH