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Hematology 110 MLT > Ch. 1 Clinical Hematology Lab Overview > Flashcards

Ch. 1 Clinical Hematology Lab Overview Flashcards

1
Q

What is Hematology

A

The study of blood cells

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2
Q

What are the 3 families of blood cells?

A

Erythrocytes - RBCs
Leukocytes - WBCs
Thrombocytes - PLTs

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3
Q

Describe the function and morphology of a RBC

A

Morphology - non-nucleated, biconcave, filled with reddish protein (Hemoglobin)

Function - transport O2 and CO2, fats, glucose, and proteins
- moves the waste to liver and kidneys

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4
Q

What are the RBC parameters of a CBC?

A 
RBC count
Hgb
Hct
MCV - RBC cell size
MCH - amount of hemoglobin / RBC
MCHC - hemoglobin / volume of RBC
RDW- red blood cell distribution width
Retic
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5
Q

What are the WBC parameters of a CBC?

A 
WBC count
Neutrophil: %  and Absolute
Lymph : %  and Absolute
Mono: %  and Absolute
EO and BASO :%  and Absolute
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6
Q

What are the platelet parameters of a CBC?

A

PLT count

MPV - mean platelet volume (like MCV)

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7
Q

How are RBC counts measured?

A
  • Microscopy and Hemacytometer

- (NOW): using automated instrumentation

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8
Q

What clinical indications are detected with a RBC count?

A

Anemia - reduced RBC

Polycythemia - increase RBC

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9
Q

How is hemoglobin measured?

A

Photometry at 540 nm with the addition of drabkin’s reagent.

-OR automated profiling instrument

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10
Q

What is hematocrit and how is it measured?

A

Hematocrit: Ratio of the volume of RBC to the volume of whole blood

Measured Manually: Centrifugation to obtain PCV

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11
Q

How is RBC morphology observed?

A

Light Microscopy and Wright-stained blood smear

Automation used to confirm results

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12
Q

Name the 5 types of leukocytes.

A

Neutrophils (SEGS) & (BANDS)

Eosinophils
Basophils
Lymphocytes
Monocytes

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13
Q

How is WBC count measured, and what are the clinical indications derived from it?

A
  • Manual : Light Microscopy and Hemacytometer
  • Automated profiling instrument

Leukopenia - decreased WBC
Leukocytosis - increase WBC

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14
Q

How is platelet count measured, and what are the clinical indications derived from it?

A

-Phase Microscopy

Thrombocytosis - increase platelets

Thrombocytopenia - decrease platelets

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15
Q

What do we look for when observe RBCs through microscopy?

A

Size, shape, color, and cytoplasmic inclusion bodies.

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16
Q

What is the function of WBCs?

A

Protect the body from pathogens, removes foreign substances and cellular waste, destroys dead/ abnormal/ worn- out cells

participate in inflammatory response

17
Q

What is the function of platelets?

A

Temporarily plugs ruptured blood vessel wall

Initiates and regulates coagulation cascade

18
Q

What is the bone marrow assay used for?

A

To analyze immature blood cell precursors

19
Q

What are cytochemical stains used for?

A

To differentiate abnormal myeloid, erythroid, and lymphoid cells

Hematology 110 MLT (2 decks)

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