Centrifuge Flashcards
A centrifuge is used to ______ gravitational separation of substances
Accelerate
Used to describe the force required to separate two phases.
Relative centrifugal force (RCF)
What is the formula for RCF?
RCF=Krn2
What is K ?
1.118 x 10 to the -5th
What is r ?
radius
What is n?
Speed of the rotor in rpm (rotation per minute)
Increase time to centrifuge a specimen if using a small radius centrifuge (T/F)
True
Increase the time to centrifuge the specimen when the Speed is increased (T/F)
False - decrease the time
SST and PST will be spun using ____ RCF for a longer time to ensure all cells have been spun to the bottom
HIGH
Urine will have a ____ RCF centrifugation to ensure cellular material is still intact
LOWER
Controlled by potentiometer which changes the voltage supplied to the motor.
Speed
Rotor head may be fixed at a ___degree angle or swing to a ____ degree angle
45 degree angle
90 degree angle
If you can hear the tubes breaking, or unbalanced it is generally best to _____the centrifuge.
Turn off and allow to come to a complete stop
The main hazard associated with centrifuge is ?
dispersion of aerosols when tube breaks
What do you need to clean the centrifuge?
Non- corrosive disinfectant