Central - Surg Path

Question 1

A.Cutting
B.Fixation
C.Staining
D.Embedding
E.Trimming
F.Dehydration
G.Clearing
H.Impregnation

sequence :)

Answer 1

B –> F –> G –> H –> D –> E —> A –> C

Question 2

enumerate two strong acids used for decalcification prior to fixation

Answer 2

formate, chlorate

Question 3

purpose of fixation

Answer 3

To preserve morphology of the tissues so that the
appearance that would be viewed under the microscope
would be as it would look like inside the body

Question 4

a. fixative agent for tissue:

b. fixation ratio for tissue:

Answer 4

a. 10% neutral buffered formalin

b. 20 vol buffer: 1 vol tissue

Question 5

For bone marrow specimen biopsy, what’s the decalcifying agent?

Answer 5

Osteomole

Question 6

purpose of dehydration is to remove moisture;what does moisture do?

Answer 6

prevent wax penetration

Question 7

used for dehydration: enum 3 substances

Answer 7

absolute alcohol, ethyl alcohol or acetone

Question 8

a. fixative agent for cytology:

b. fixation ratio for cyto:

Answer 8

a. 25% alcohol

b. 1:1

Question 9

why is clearing done?

Answer 9

alcohol and wax are immiscible

Question 10

what is used for clearing?

Answer 10

chloroform/xylene

Question 11

what happens if there is too much clearing?

Answer 11

brittleness