Central Dogma (T) Flashcards
Main laboratory ware used in dilution plate counts
petri dish
Microorganisms produced per unit substrate utilized
Growth Yield
CFU
Colony Forming Units
Measure particle size distribution of cells in a culture.
Particle Counter
A measure of the amount of light that passes through a turbid sample.
Optical Density
Typically used as seed in batch culture.
inoculum
Oxygen solubility in water at 25 deg C and 1 Atm
7 PPM
Typical cause of limited oxygen transfer rate
Stagnant liquid around bubble
Affect the transport of certain nutrients in and out of the cell
ionic strength
Dissolved gas toxic to some organisms at high cone
dissolved carbon dioxide
Catabolism of cellular reserves for continued maintenance and energy.
endogenous respiration
Cell growth equation.
mu = 1/X * d/dt (X)
Cell produce secondary metabolites
Stationary Phase
Adaptation to new environment
Lag Phase
Cell physiology changes to favor survival over growth
Deceleration Phase
cryptic growth occurs
Stationary Phase
extended by low temperature
Lag Phase
Commonly molded as a 1st order process with respect to biomass
Death Phase
Growth balanced
exponential growth phase
Some portion of cells remain viable for a long time, but are altered
Death Phase
No dependence on substrate concentration
exponential growth phase
Caused by either build-up of toxic products or depletion of substrate
Deceleration Phase
Primary metabolites produced
exponential growth phase
No cell division occurs
Stationary Phase
