Cellular Biology Flashcards
What is the difference with resolution and
magnification?
Magnification is the ability to make an object seem larger than it actually is while resolution is the ability to distinguish detail.
What is numerical aperature?
This shows the level of resolution the higher it is the greater the resolution
what are the different power levels of the microscope?
x4, x10, x40, x100 oil
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What is a cell?
this is a basic unit of organisation or structure of all living matter
what do all true cells share?
Genes (DNA, RNA), Plamsa membrane, Metabolic Machinery
What are the structures that all true cells share?
Ribosomes, Plasma membrane, ctyoplasm
What are the beliefs of the Cell theory?
- All living organisms are made up of one of more cells and cell products
- All metabolic reactions in unicellular and multicellular organisms take place in cells
- Cell originate from other cells
- The cell is the smallest clearly defined unit of life.
What are the domains that all living organism are classified into?
Archaea, Bacteria, Eukarya
How do prokaryotes reproduce?
Binary Fission
explain the process of binary fission?
The cell replicates its DNA. Then the cytoplasmic membrane elongates separating DNA molecules. Then the cross wall forms separating the daughter cells.
what are the different ways genetic variation can take place in prokaryotic cells?
Transformation. Transductions. Conjugation. Transposable element.
How does transformation take place?
This is when a bacterial cell with is own chromosomal DNA can pick up a plasmid from the surrounding environment
How does Transduction take place?
Viruses release its DNA into a host cell and the host cell makes copies of the viral DNA viral protein will then be made. The bacterial DNA and the viral DNA can combine with the viral protein. therefore the next time a viral does and releases its DNA into another cell the bacterial da will get spliced into the host chromosomes.
How does conjugation take place?
Bacteria that contains f+ plasmids (donor cell) will connect its pills with a f- (recipient cell). the donor cell will introduce its f+ plasmid to the new cell making it into new donor cell.
How does transposable element form of genetic variation take place?
fractions/pieces of DNA molecule that can be transposed, removed and moved into another DNA molecule
What colour are gram positive cells?
purple
What colour are gram negative cells?
pink
what are the different eukaryotic kingdoms?
Kingdom Fungi
Kingdom Plantae
Kingdom Animalia
Kingdom Protista
What does law of instant volume mean?
Cell volume remains constant for a particular cell type. and is independent of the size of the organism…liver cells in the liver of a cow and the liver of a humans is the same size
What is the endosymbiotic cell theory?
Infolding of plasma membrane allowed for development of internal structures.
aerobic bacteria engulfed.
endosymbiotic relationship would’ve formed with the bacterium host using oxygen, various process and producing atp and the cell protecting the bacteria.
became co-dependent bacteria lost its functions and transferred genes to the host cell.
Host engulfed photosynthetic bacterium and can now carry out photosynthesis.
what is the result of the endosymbiotic process?
the development of Mitochondria and chloroplast
Name single membrane organelles
Golgi, lysosomes, peroxisomes, vacuoles, vesicles
Name double membrane organelles
Nucleus, Mitochondria, Chloroplast
Why do organelles need membranes?
Structure- plasma membrane is amphipathic - has both hydrophilic and hydrophobic parts which allows it to be selectively permeable.
Function- allows endocytosis and exocytosis to take place. also allows compartmentalization
What are the advantages of organelles?
- Division of labour between compartments
- Molecules kept away from each other to prevent inappropriate reactions and allow reactions to coexist at the same time
- Reactions can proceed efficiently
- Membrane can regulate uptake and explosion of materials
- Different parts of a multistep process can occur at the same time.
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Name structures that aren’t true organelles?
Ribosomes, Cytoskeleton, Extracellular Matrix
Differences with (Prokaryotes) Bacteria Archaea and Eukaryotes?
Cell structure Bacteria. Archaea. Eukaryotes
Size. 0.5-1. 0.5-1. 5-20
nucleus. no no yes
Chromosome. 1 1 1>
shape circular circular linear
haploid. haploid diploid
histones no yes yes
cell division binary. binary mitosis/meosis
what are the functions of the plasma membrane?
- Maintains integrity
- regulates movement of metabolic reactions to other cells
- regulates influx and efflux of materials
- compartmentalisation
- Osmoregulation
- Antigen to antibody recognition
- Tissue formation (cell adhesion)
- cell to cell recognition
- allows cell/ organelle motility
What are the compositions of the plasma membrane?
Lipids, Proteins, Cholesterol, Carbohydrates
What are integral proteins?
They are responsible for channeling ions, signalling and attachment points.
What are peripheral proteins?
These are usually attachment points for integral proteins
Whats the importance of the lipid component of the plasma membrane?
This helps to maintain fluidity and integrity of the plasma membrane. Also it is the first barrier against hydrophilic substances.
what happens to the bilayer at low temperatures?
The bilayer becomes tightly packed. (gel phase)
What happens to the bilayer at high temperatures?
The bilayer melts (fluid phase) movements allowed
What is the importance of cholesterol to the plasma membrane?
- Makes the bilayer less deformable
- Decreases it permeability to small water soluble molecules
- Prevents crystallisation of hydrocarbons and phase shifts in the membrane
- without cholesterol an animal cell would need a cell wall
What is the importance of carbohydrates in the plasma membrane?
This can be attached to lipids (glycolipids) or proteins (glycoproteins). they can also be protective, insulants and sites of receptor binding.
Whats the importance of proteins in the plasma membrane?
- movement of materials
- Diffusion. Active transport, osmosis, facilitated diffusion
- Receptors (glycoproteins)
- Enzymes systems
- Ionospheres (chemicals that irreversible binds ions
What is the functions of the cytoplasm?
Energy transfers. Cell expansion. Growth.
Replication of organelles. Gives cell its shape.
Home of the cytoskeleton.
Storage place for chemical substances.
What does the cytoplasm comprise of?
Cytoplasmic matrix/ cytosol.
colloidal suspensions.
inclusions: Crystalline or soluble.
Constitute the microtrabecular lattice
what is colloidal suspension?
This is when particles dont settle but stay suspended (cytoplasm)
What is the cytoplasmic matrix/ cytosol?
This is a semi-transparent fluid in which organelles are suspended and makes up 80% of the cell. Mainly water based.
what are inclusions?
These are chemical substances that store nutrients , secretory products and pigments granules.
Name energy transducer organelles
Mitochondria and chloroplast
Name information processor organelles
Nucleus and ribosomes
Name the different types of organelles
Secretory. Storage. information processors.
energy transducers.
What are the types of endoplasmic reticulum?
Rough endoplasmic reticulum.
smooth endoplasmic reticulum
What is the function of the rough endoplasmic reticulum?
- protein synthesis with associated ribosomes and mRNA
- Segregation of newly synthesised proteins
- Chemical modification of proteins
What is the function of the smooth endoplasmic reticulum?
- modifies chemicals imported into the cell
2. Lipid and steroid synthesis
what is the function of the Golgi complex?
- Receives synthesise proteins from the endoplasmic reticulum
- concentration, modification and final packaging of proteins.
- Synthesis of cell wall polysaccharides
- Stores proteins and enzymes of the cell
- Forms secretory vesicles (storage and transport) and lysosomes (digestive processes of the cell)
What are the function of lysosomes?
- Degradation of cell organelles
- maintains the balance between synthesis, degradation and recycling of of cellular products
- A major mechanism in which a starving cell may reallocate nutrients from unnecessary processes to more essential processes
what is the function of peroxisomes?
- Detoxification.
- The breakdown of fatty acid molecules to 2 carbon fragments and converted acetyl- CoA and fed back into cellular respiration
What is gluconeogenesis?
This is when sugars are synthesised from fatty acids until the seedling is mature enough to produce them by photosynthesis
what is the function of the vacuole?
Provide turgor in plants. Waste Disposal and Recycling. Storage of toxins. Pigmentation. Germination.
Attract pollinators.
what are plastids?
This is any pigmented cytoplasmic organelle found in plant cells and protist.
What are kinds of plastids?
Chromoplast and Leucoplasts
What is the function of chromoplast?
Attraction of pollinators and dispersal agents through floral and fruit colours
what is the function of leucoplast?
Specialised for storage
What is it important for cells to be small?
This allows for there to be a larger surface area that allows greater rate of exchange of resources and removal of waste products, while large cells have high rate of chemical activity but low rate of exchange
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What are the structures of prokaryote?
Plasma membrane, cell wall, capsule, cytoplasm, ribosomes, nucleoid, plasmid.
what are the different types of eukaryotic cell shape?
fixed - paramecium
variable - white blood cells, amoeba
What maintains the eukaryotic cell shape - unicellular?
tough plasma membrane, exoskeleton
What maintains the eukaryotic cell shape - multicellular ?
cytoskeletons, plasma membrane, adjoining cells, external environment,
what is the function of the mitochondria?
This is the powerhouse of the cell. produces energy through cellular respiration like: Krebs cycle, electron transport system, B- oxidation of fatty acids.
What are the functions of the nuclear pores in the nucleus?
- communication with cytoplasm
- transport of ribosomes, nucleotides and proteins
- Associated with rough endoplasmic reticulum
What are the functions of the nucleus?
Site of DNA storage, genetic control of metabolism, produces ribosomes and RNA
what are histones?
these are proteins involved in a range of activities including DNA replication and gene expression.
What are chromosomes?
These are highly condensed, visible strands of chromatin.
what are the functions of the extracellular matrix?
- Cellular organization in tissues (binding)
- Provide storage and anchorage for cells
- Segregating tissues from one to another
- Cell signalling (regulating intercellular communication and cell behaviour
- Privides lubrication in joints
- Influences the processes of growth and repair
- Influences the physical properties of the tissues
what are cell adhesion molecules?
These are cell surface proteins that binds with other cells or extracellular matrix
What are the principle classes of cell adhesion molecules?
- Cadherins - calcium dependent adhesion molecules
- Immunoglobulin superfamily - attached to the membrane of the effector B cells
- Selectins - initial attachment of leukocytes during inflammation
- Mucins - coat many epithelial surfaces and is secreted into fuilds such as saliva e.g lubrication, cell signalling
- Integrins - mediate cell to cell and cell matrix interaction and communication
What are cell junctions?
These are protein complexes that allow contact, adhesion and communication between neighbouring cells, cells and the extracellular matrix.
what are the types of Cell Junctions?
Tight junctions
Desmosomes
Gap/ Communicating junctions
Whats the structure and function of the middle lamella in the plant cell wall?
This adheres cell wall of adjoining cells and is formed from cell plate during cell division. It is made up Pectin- calcium and magnesium
What are the different cell transport mechanisms?
Molecular movement
transmembrane movement
mediated transport
Phospholipid movement
what is molecular movement?
This is the movement of molecules from an area of high concentration to an area of low concentration. this takes place via random kinetic (brownian) movement.
What are factors that affect the rate of movement of molecules?
size of particle, electrical charge, concentration gradient and thermal energy
What are the types of phospholipid movement?
Rotational movement - spinning in place
Lateral movement - moves side to side (rapid) allows fluidity of the cell
Transverse movement - phospholipids exchange places with each other. like top switch with bottom
What are the types of Passive transport processes?
Simple diffusion
Osmosis
Facilitated diffusion
What are the two types of active transport processes?
Endocytosis and exocytosis
What can DIFFUSE through the lipid bi layer?
Gases (Co2, N2, O2), Small uncharged polar molecules (ethanol), water,
What cannot DIFFUSE through the lipid bilayer?
Large polar uncharged molecules (Glucose), Ions (K+, Mg2, Ca2+, Cl-) Charged polar molecules (amino acids, ATP)
What affects osmosis movement?
Hydrostatic pressure and osmotic concentration
What is bulk flow?
Bulk flow is a process used by small lipid-insoluble proteins to cross the capillary wall.
What is solvent drag?
This is when solutes are dragged along with the passing water.
What are the types of proteins in facilitated diffusion?
Passive proteins and Carrier proteins
Whats the difference with passive and carrier proteins?
Carrier proteins need to be glucose activated and allows on molecule at a time while passive proteins just stay open.
What is mediated transport?
This is the transport of molecules via pumps or channels.
What are the different kinds of channels?
Ion channels and Gated channels
What are characteristics of Ion channels?
- channels can be highly selective e.g some will transport -ve but not +ve
- They exist in open and closed states
- Transitions between open and closed states are regulated
What are the different classes of ion channels?
Ligand-gated channels - open and close in repsonse to the binding of specific chemicals
Voltage gated channels - open and close in response to the electrical potential across the membrane in which they are found.
What are the types of gated channels?
- mechanical gated channels
2. Voltage-gated channels
What are mechanical gated channels?
mechanical stimulation of the operation ion channels e.g inner ear sensory cells of the cochlea (sound waves)
What are Voltage-Gated channels?
operates in neurons and muscle cells. channels open and close in response to changes
What are uniports?
these are carrier proteins that transport a single solute form one side of the membrane to another
What are coupled transporters?
these are carrier proteins that transport two or more solutes from one side of the membrane to another
What are symports?
these are carrier proteins that transport two different substances in the same direction
What are antiports?
these are carrier proteins that transport two different substances in opposing directions.
Explain primary and secondary active transport.
active transport utilizes membrane pumps.
Primary directly harnesses ATP while secondary indirectly harnesses ATP
What is pinocytosis?
This is the ingestion of fluid material
What is phagocytosis?
This is the ingestion of solid material
What is vacuolisation?
This is the formation of vacuoles or vacuole-like structures. his usually takes place because of endocytosis when foreign objects after entering the cell would be surrounded by a vacuole/vesicle.
What is movement?
This is the use of chemical energy to apply force to a mass or change in position of a part or organ of an organism.
What is locomotion?
This is the change in position of a whole organism
What are the types of locomotion
Amoeboid, Ciliary/flagellar, Muscular
What are the types of intracellular movement?
Vesicular movement, Cytoplasmic streaming, Cell division
what are the components that facilitate intercellular movement?
Microfilaments, microtubules, motor proteins
what is the cytoskeleton?
This is a collection of thin long fibres which maintains cell shape, support cellular movement (positions and moves organism) facilitate the transport of substances in out and around the cell,
What are the components of the cytoskeleton?
Microfilaments, microtubules, intermediate filaments
What is the Microtubule organising centre?
This is the area near the nucleus where microtubules are assembled
What are the two types of microtubule organising centres?
Basal bodies - associated with the cilia and certain intercellular junctions in epithelial cells
Centrosomes - associated with animal cells
What are the functions of microfilaments?
Maintain shape
Changes in cell shape (animal cytokinesis)
muscular activity in concert with myosin filaments
What are the functions of intermediate filaments?
Stabilise cell structure (in desmosomes)
resist tensile forces
provide anchorage for cytoskeleton (in the base of the microvilli)