Cells - The Fundamental Units of Life Flashcards

1
Q

what is the most basic unit of any organism

A

cells (smallest unit of life)

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2
Q

what makes a cell alive

A

can divide, grow and take energy from environment
grow and create copies of themselves

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3
Q

definition of cells

A

membr enclosed units willed with concentrated aqueous solution of chemicals and can grow and create copies

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4
Q

what is the form and function principle

A

cells are specialized and take on a form that allows them to do their function

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5
Q

what is the language of cells

A

ATCG: DNA / chemical composition

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6
Q

what is the central dogma

A

nucleotides in a segment of DNA (gene) transcribed into RNA
translated into amino acids
forms a protein

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7
Q

how is life an autocatalytic process

A

DNA/RNA provide sequence info used to synthesize prots
prots have catalytic funcitons to synthesize DNA/RNA/prots
creates a self-replicating system

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8
Q

what is cell theory

A

all living organisms are made up of 1 or + cells
all cells arise from other preexisting cells

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9
Q

if we all cells come from preexisting cells, how do we evolve

A

mutations: change in amino acid sequence in DNA

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10
Q

how do mutations explain evolution

A

if mutation is beneficial, its kept
if not, not kept when possible

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11
Q

what are the different types of mutations

A

silent mutation: no effect on function
deleterious: changes the function
beneficial: will be kept, explains how we adapt to environments

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12
Q

are viruses alive

A

no, they can grow and divide on their own, but need a host, cant supply their own energy from the environment

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13
Q

who are the 2 pioneers in cell microscopy

A

Robert Hooke
Antoni can Leeuwenhoek

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14
Q

who was the first to draw a living plant cell and when and how

A

1880, Eduard Strasburger
hair cell from Tradescantia flower
can see DNA condensing into chromosomes

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15
Q

who worked on cell theory and what else did they do

A

Schleiden and Schwann 1838-39
systematic study of plant and animal cells with light microscope

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16
Q

what are characteristics of a light microscope

A

magnify object up to 1000x
method: difraction of light
light is focused into specimen by lenses in the condenser
resolution: 200nm
can look at: cell division

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17
Q

what is the limit of a light microscope

A

resolution/resolving power: min distance btw 2 adjacent objects for them to be distinguished as 2 separate objects

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18
Q

what affects the resolution

A

wavelength, (400-700nm)

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19
Q

what is the smallest resolution you can get with a light microscope

A

2 objects 200nm apart, cant see less than that

20
Q

what are the characteristics of a transmission electron microscope

A

method: beam of electrons
resolution: a few nm
requirements: very thin specimen
stained with electron-dense heavy metals
can look at: DNA molecule

21
Q

what is the order through which the light goes through in a light microscope

A

light source, condenser lens, glass slide, specimen, objective lens, tube lens, eyepiece, eye, retina

22
Q

what is the order through which the beam of electrons goes through in a TEM

A

electron gun, condenser lens, specimen, objective lens, projector lens, viewing screen (photographic film)

23
Q

what are the characteristics of a scanning electron miscroscope

A

method: scatters electrons off the surface of samples
can look at: structures on the surface

24
Q

what order does the electrons go through in SEM

A

electron gun, condenser lend, beam deflector, objective lens, specimen + scan generator, video screen, detector
limitation: only surface, cant see inside

25
what can allow you to see intracellular strucutres
stains/dyes
26
how does fluorescence microscopy work
electrons absorb light, molecules are excited, going back down, they emit light you can see
27
when to use fluorescence microscopy
see intracellular components (better resolution)
28
what are the steps for labelling cells
permeabilization, labelling, washing, recovery, observe
29
what are the pieces of a fluorescent microscope
light source horizontal, beam-splitting mirror, objective lens, object, then goes back up, into eyepiece
30
what is confocal fluorescence microscopy
multiple slice of an image, creates 3D image
31
what are the parts of a confocal microscope
light source, source pinhole that diffracts the light, beam-splitting mirror, objective lens, focal plane, detector
32
what is super-resolution microscopy (STED)
2 lasers: fluorescence can be on/off with a laser creates a more focused image
33
what are the components of a STED microscope
green excitation laser, red depletion laser
34
how small can you see with a STED microscope
almost the actual size of a microtubule (25nm diameter)
35
starting from a thumb, say what instrument you need to see increasingly small components in the thumb
Thumb: eyes skin (2mm): eye skin cells (0.2mm): light microscope cells (individual) (20um): LM organelles (mitochondria) (2um): LM organelles (ribosomes) (0.2um): LM molecules (proteins) (20nm): super-resolution fluorescence microscope molecules (cluster of atoms) (2nm): S-R FM atoms (individually) (0.2nm): electron microscope
36
eukaryotic vs procaryotic
Eu: DNA in nucleus, bigger, cytoplasm has specialized structures: organelles Pro: DNA in cytoplasm, no nucleus, smaller, internal structures not organized in compartments
37
which is most diverse and numerous on earth
procaryotes
38
what are the 2 domains of procaryotes
bacteria and archaea
39
what are some characteristics of procaryotes
small, divide in 2 rapidly, inhabit diverse environments
40
what energy source do prokaryotes need
dont need O2 C (CO2) ; N (N2) ; H (gas), Light (Sun)
41
what are the components of a bacterial cell E coli
outer membr ; cell wall ; plasma membr. ; cytoplasm
42
where is the DNA in bacteria
light area (center) of cytoplasm
43
what bacterias are photosynthetic
anabena cylindrica: fix N, fix CO2 through ph.syn., become resistant spores that survive + phormidium laminasum: ph.syn. in intracellular membr
44
where does a sulfur bacterium get its energy from
oxydizes H2S to produce sulfur (yellow in microsc) can fin C in the dark beggiatoa (lives in sulfurous env)
45
how are archaea and bacteria alike/diff?
alike: structurally the same diff: distantly related (DNA)
46
characteristics of archaea
can survive hostile env like: [brine], hot acid(volcano), airless depths of marine sediments, sludge of sewage treatm plants, pools beneath frozen surf of antartica