Cells background information Flashcards
Outline Cell Membranes
THE cell membrane - around the outside of all cells which protects the cell and controls movement of substances in and out of cells.
•Other membranes surround most organelles including the nucleus
•Membranes are composed of a phospholipid bilayer – a type of fat
Cell Wall (Plants Only!)
•Structure around cell membrane.
•It strengthens plant cells and makes them rigid and tough.
All cells contain the following organelles:
- Nucleus
- Cell Membrane
- Mitochondria
- Lysosome
- Fluid filled Vacuole(large & permanent in plants)
- Cell Wall (plants only)
Outline of the Nucleus
•Large dark staining region of an animal or plant cell (not in bacteria).
•It contains all the genetic information for the cell (DNA) which is packaged in chromosomes
•The nucleus of any cell contains the genetic information to allow the growth of that cell.
•The genetic information is contained in a substance called DNA
•The DNA is formed into “filing cabinets” called chromosomes.
Deoxyribonucleic Acid
What is a chromosome
A chromosome is a long molecule of DNA contained within the nucleus of a cell.
It carries the genetic information (genes) of an organism.
Outline on the chromosomes
Autosomes
Heterosomes
- The chromosomes you inherit determine what you look like and so much more!
- An autosome is a chromosome other than a sex chromosome
- Sex chromosomes are a chromosome involved in sex determination (e.g. X and Y chromosome)
- Sex chromosomes are referred to as heterosomes.
- They are not identical in shape and size. E.g chromosome 23…X Y
Outline on Karyotype
set of all chromosomes from an organism
It can be difficult to count all the chromosomes when they’re all jumbled up, so genetisits like to take a photo and cut each one out.
Then they paste them onto another sheet, arranging them in pairs, from biggest to smallest matching centromere position.
This is ‘karyotyping’.
Steps involved in Removal of chromosome
- Break cell wall – blender
- Break cell membrane – detergent
- Unwrap DNA coils – meat tenderiser
- Separate DNA from water - Alcohol
Outline on DNA molecules
All living things contain Genetic material (material passed on from one generation to the next) in the form of a nucleic acid.
Nucleic acids are polymers – that is they are made up of repeated units.
Each unit (a monomer) contains:
•A phosphate group
DNA molecules
• A pentose sugar ( a sugar with 5 carbons in it!)
• A nitrogenous base (a molecule with nitrogen in it!)
DNA Monomer (Nucleotide)
Structure of DNA
The phosphate and sugar form string binds called a sugar phosphate backbone
When two nucleoids attach he result is called a base pair
1.Adenine (A) always pairs with Thymine (T).
2.Cytosine (C) always pairs
with Guanine (G).
What is complementary sequence
The sequence on the matching strand is therefore called the Complementary Sequence.
A G T C
DNA part 2
- Hydrogen bonds between nucleotides can be broken by enzymes
- This “unzips” the DNA strands
- The strand can then be copied into an mRNA molecule (messenger Ribonucleic Acid)
- This is called Transcription
What is mRNA
•Messenger RNA is like a photocopy of DNA
•It is only a SINGLE stranded molecule (not a double helix like DNA)
•It has three bases the same as DNA: A, C & G
•Instead of Thymine (T), it has a nucleotide called Uracil (U).
•mRNA can leave the nucleus and DNA can’t to keep the original coding safe!
Why is this important? - protein synthesis.
•This mRNA then leaves the nucleus and enters the cytoplasm.
•It attaches to a ribosome (protein making machine) and the mRNA is translated into a polypeptide chain which is really just a protein.
•The building blocks of proteins are called AMINO ACIDS
•This process needs help of another molecule called tRNA (t is for TRANSFER) which picks up the amino acids in the cytoplasm and helps join them to the chain being formed at the ribosome
How does the tRNA know which amino acid to bring?
The nucleotide bases on DNA code for individual amino acids in 3 letter words
•When transcribed to mRNA these get called CODONS
•tRNA has matching ANTI-CODONS (remember base pairs) and each different type of tRNA is for a specific amino acid
What is the difference between Transcription & Translation
Transcription is copying DNA to RNA
Translation is where RNA is used to code for proteins!
MITOSIS
- Produces TWO cells per division
- New cells have exactly the same number and type of chromosomes as the original
- They are IDENTICAL and have 46 chromosomes each (in humans)
- This is in SOMATIC (normal body) cells