Cells as the Basis of Life (Tillie) Flashcards

1
Q

What are cells?

A

Cells are the structural unit of living things, known as the “building blocks” that make up organisms, and they are also functional units as organisms are made up of many cells, therefore when we refer to what they do we also refer to cells

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2
Q

What is Cell Theory?

A

Cell Theory is a unifying concept as it unifies all living things as they are the basic structural unit and functional unit of life.

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3
Q

Describe Cell Theory

A

includes the idea that all cells come from a previous cell that contain hereditary information, all living things are complex and have organised structures, cells take in energy from their surrounding environment and use it, cell’s have an internal composition that is chemically different from external environment, and cells respond to stimuli, reproduce themselves and grow.

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4
Q

Describe what the cell membrane is?

A

The cell membrane, also called plasma membrane, separates the cell from its surroundings and controls the exchange of materials, including nutrients and wast, between the cell and its environment

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5
Q

Describe the fluid mosaic model?

A

The model proposes that the cell membrane is a fluid mosaic as it is composed of a phospholipid bilayer with protein molecules embedded in and through the layer at various point.
Fluid: as phospholipids are constantly moving
Mosaic: proteins embedded in membrane

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6
Q

What are the differences between prokaryotic and eukaryotic cells?

A
  • Size: prokaryotes are smaller in size compared to eukaryotes
  • DNA: the DNA is circular in prokaryotes and linear in eukaryotes
  • nucleus: there is no nucleus in prokaryotes and is a nucleus in eukaryotes
  • internal organisation: little internal organisation in prokaryotes and high level internal organisation in eukaryotes
  • location of chromosomes: DNA in prokaryotes is in cytoplasm, DNA in eukaryotes in nucleus
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7
Q

What is the structure and function of nucleus?

A

Structure: nucleus is composed of two membrane layers called the nuclear envelope and it contains nuclear pores lined with proteins
Function: controls the activity of the cell and stores genetic information (DNA)

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8
Q

What is the structure and function of the nucleolus

A

Structure: a circular region not bounded by membrane in the nucleus that is composed of DNA and proteins
Function: produces and assembles cell ribomosomes

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9
Q

What is the structure and function of mitochondria?

A

Structure: has an outer membrane and an inner membrane which is folded to form structures of cristae
Function: involved in the latter stages of aerobic respiration using O2 and glucose to release energy to be stored in ATP

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10
Q

What is the structure and function of chloroplast?

A

Structure: Only found in plants and are a type of plastid. It has 2 outer membranes and inside there is a system of flattened sacs called thylakoids which are arranged into stacks of grana (singular granum). Fluid called stroma surrounds the membrane out sacs
Function: photosynthetic pigment, chlorophyll, is stored in thylakoids to carry out photosynthesis

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11
Q

What is the structure and function of vacuole?

A

Structure: a fluid filled space bounded by a membrane. Most plants contain large central vacuole.
Function: maintaining balance of water and salt for the cell and is a place to store organic compounds such as proteins and sugars and inorganic compounds such as ions (potassium and chloride)

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12
Q

What is the structure and function of golgi body?

A

Structure: sticky of flattened sacs made of smooth membranes
Function: involved in the packing and secretion of proteins and carbohydrates manufactured by the cell

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13
Q

what is the structure and function of endoplasmic reticulum?

A

Structure: system of membranes that extends throughout the cytoplasm from the nuclear envelope to cell membrane. Forms intricate network of pathways
Function: involved in transport of materials
RER structure: attached ribosomes
Function: site of protein and membrane synthesis
SER: no attached ribosomes
Function: involved in metabolic processes such as lipid synthesis and carbohydrate metabolism

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14
Q

What is the structure and function of ribosomes?

A

Structure: made up of RNA and protein, and is enclosed by a membrane
Function: site of protein and membrane synthesis

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15
Q

What is the structure and function of lyosome

A

Structure: vesicle, membrane enclosed containing digestive enzymes
Function digests macromolecules

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16
Q

Describe what prokaryotic cells are

A

A single-cell organism that lacks a nucleus are other membrane bound organelles and belongs to domains of bacteria and archaea

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17
Q

Describe what eukaryotic cells are

A

An organism that possess a clearly defined nucleus and membrane bound organelles. They can range from single-cell organisms to complex multicellular organisms like animals and plants

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18
Q

What is the structure and function of the cytoskeleton?

A

Structure: network of fibres throughout cytoplasm, it is made up of three main components microfilaments, intermediate filaments and microtubules. The components are made up of protein moleules that can be assembled to form long filaments and hollow tubules.
Function: gives cell shape, involved in cell movement, holds organelles in place and provides cell strength

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19
Q

What is the structure of plant cells

A

Cell wall, cell membrane, nucleus, nucleolus, mitochondria, chloroplasts, vacuoles (large central), Golgi body, endoplasmic reticulum, ribosomes, lysosomes and cytoskeleton

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20
Q

What is the structure of animal cells

A

Cell membrane, nucleus, nucleolus, mitochondria, vacuoles (small), Golgi body, endoplasmic reticulum, ribosomes, cytoskeleton

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21
Q

What do cells require?

A

Cells require inputs of suitable forms of energy, including light energy or chemical energy in complex molecules

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22
Q

What are autotrophs?

A

Organisms that make the energy rich compounds they need from simple inorganic susbstance, and energy for synthesis reactions, commonly from sun, using physical energy

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23
Q

What are heterotrophs

A

Organisms that cannot make energy rich compounds their self, therefore they rely on the intake of nutrition from other sources of organic carbon, using chemical energy

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24
Q

What is photosynthesis and why is it important?

A

Photosynthesis is the process when light energy is used to synthesis complex organic compounds from simple organic substances. It is a fundamental chemical process that provides photsynthic autotrophs with food and also takes carbon dioxide out of the atmosphere by replacing it with oxygen

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25
What is the equation for photosynthesis
6CO2 + 6H2O - light/chlorophyll --> C6H12O6 +6O2
26
what is the chemical equation for aerobic respiration
C6H12O6 +6O2 --------> 6CO2 + 6H2O
27
What is the worded equation for aerobic respiration
Glucose + Oxygen -----> Carbon dioxide + water
28
What is the worded equation for photosynthesis
Carbon dioxide + water -----> Glucose + oxygen
29
What is anerobic respiration?
respiration that does not require oxygen to generate energy from glucose
30
Why is anerobic repsiration important in plants and yeast
to provide them energy when oxygen is unavailable
31
why is aerobic respiration important
as it converts oxygen and food (glucose) into energy that eukaryotes require to survive
32
what is the worded equation for fermentation in plants and yeast
glucose -------> ethanol + carbon dioxide
33
What is chemical equation for fermentation in plants and yeast
C6H12O6 ---------> 2C2H5OH + 2CO2
34
why is anerobic respiration important in animals
to produce energy for the cells when undergoing exercise, ensuring that blood is being delivered to cells under stress
35
What is the worded equation for fermentation in animals
glucose -------> lactic acid
36
What is the chemical equation for fermentation in animals
C6H12O6 ------->2C3H6O3
37
How much energy is released through aerobic respiration
36 ATP yield per glucose
38
how is energy released in aerobic respiration
Through the breakdown of glucose in the presence of oxygen as the energy in the reactants is greater than the energy in the products.
39
how much energy is released in fermentation
2 ATP yield per glucose
40
how is energy released in anerobic respiration
through the breakdown of glucose molecule through glycolysis, in which a glucose molecule is broken down into two pyruvate molecules, releasing electrons in the process and producing two ATP molecules, the energy of the cells.
41
What happens to energy when new bonds are formed
The formation of chemical bonds releases energy
42
what happens to energy when chemical bonds break
When the chemical bonds that hold atoms together are needed to be broken energy is required
43
is energy required or released when chemical bonds are broken
Required
44
is energy required or released when new bonds are formed
Released
45
why do cells require input of matter
To provide them with the materials needed to grow and carry out life processes
46
why do cells require output of matter
remove cell waste
47
What are the inputs in autotrophs
Oxygen, carbon dioxide, nitrates, nitrites, phosphates, calcium, inorganic nutrients
48
What are the outputs in autotrophs
Oxygen, carbon dioxide, ethanol
49
What are the inputs in heterotrophs
Oxygen, nitrates, nitrites, phosphates, calcium, inorganic nutrients, organic compound
50
What are the outputs in heterotrophs
Carbon dioxide, lactic acid, urea
51
What is diffusion
Occurs through the membrane bilayer where movement of one substance from an area of high concentration to an area of lower concentration until equilibrium is reached, the transport is passive
52
What is facilitated diffusion
Substances moved from high concentration to lower concentration through the involvement of proteins embedded throughout membrane, passive transport
53
what is osmosis
The diffusion of water through passive transport to maintain salt and water balance and cell shape
54
what is active transport
Is the movement of substances against the concentration gradient with the input of energy and carrier protein
55
what is endocytosis
When cells take in particles or large molecules by enclosing them in a membranous vacuole, being two main types phagocytosis (intake of particles when the membrane invaginates in the vicinity of the particle enclosing it into a vacuole then breaks away from membrane into cytoplasm) and Pinocytosis (intake of liquids or large molecules unto tiny vesicles that form on cells cerface
56
what is exocytosis
Is the secretion of material produced by the cell involving the packing of materials into a vesicle that migrates to the membrane to be fused and release contents)
57
How does the structure of a membrane facilitate different processes of movement
The phospholipids of plasma membranes are amphipathic: they have both hydrophilic (water-loving) and hydrophobic (water-fearing) regions. The hydrophobic core of the plasma membrane helps some materials move through the membrane, while it blocks the movement of others.
58
What is the role of transport proteins, including channel and carrier proteins
Channel proteins: bind certain ions/molecules and Carrie’s them through membrane Carrier proteins: change shape as they bind to specific molecules to assist in transporting them from one side of the membrane to another
59
How is the exchange of materials affected by surface-area-to-volume ratio
As cell size increases its surface area to volume ration decreases, meaning less relative surface area is being exposed to the environment compared to cell volume Thus if the cell has to low of a surface area to volume ratio the exchange of material with extra cellular space decreases
60
How is the exchange of materials affected by concentration gradients
In diffusion and osmosis particules move with the concentration gradient from high to low, thus the greater the difference in concentration the rate of exchange increases
61
How is the exchange of materials affect the physical and chemical nature of the materials being exchanged
The type of particle exchanged is important as small uncharted particles such as O2 and CO2 can pass through the membrane easily via diffusion and larger/charged particles such as glucose and ions require assistance from channel proteins, carrier proteins or endocytosis
62
What is cell metabolism and why is important
As cells need to synthesis a large number of compounds such as proteins, carbohydrates, lipids, and nucleic acids and break down compounds such as glucose provide
63
What are biochemical processes in cells influenced by
by the nature and arrangement of internal membranes and the presence of specific enzymes
64
how does the structure of the internal membranes of mitochondria facilitate biochemical processes
The cristae provide large surface area for the attached ent of enzymes such as ATP synthesis that plays an important role in aerobic respiration. The folded inner membranes of the mitochondria are the main site of ATP production
65
how does the structure of the internal membranes of chloroplast facilitate biochemical processes
Thylakoids contain photosynthetic pigments that are able to trap light energy for photosynthesis
66
Explain the importance of regulated steps in metabolic pathways
Ensures the reaction does not occur too rapid, preventing the cell burning up and maximises the amount of energy stored in ATP molecules during the process
67
Explain the importance of steps producing intermediate compounds in metabolic pathways
Allows a starting point for other reactions
68
Why, at each step, does energy release in metabolic pathways
So energy can be trapped by energy molecules like ATP
69
Why are specific enzymes required at each step in metabolic pathways
To decrease the time chemical processes occur as if not them chemical processes would occur to slowly or not at all
70
What are biochemical processes in cells influenced by
Presence of enzymes, thus affected by temperature, pH, the presence of inhibitors, concentration of reactants and concentration of enzyme
71
What are possible benefits of the chemicals used by humans
- development of drugs and vaccines - cleaning agents (remove microorganisms from surfaces - preservatives - biotechnology applications Etc..
72
What are the possible harmful effects of chemicals used by humans
Harmful effect depends on type of chemical, exposure level, exposure duration and individual susceptibility - respiratory issues - carcinogens - reproduction issues - skin and eye irritation
73
Why does the amount of DNA double in a cell before division
In multicellular organisms cells need to be continually replaced, thus each of the new cells must relieve a copy of the genetic information stored in DNA. This caused DNA in a cell to be replicated before the cell divides to ensure that when the cell divides each new cell will recieve the same amount of DNA containing identical information
74
describe and explain the process of binary fission in prokaryotes
- The Dna is formed into a single double helix ring called nucleotide - DNA duplicated before division - both nucleotides attach to the cell membrane by proteins near the middle of the cell - the cell grows and the loops of DNA (chromosomes) move apart - the cell pinches across the equator giving rise to two identical daughter cells
75
describe and explain the process of mitosis in eukaryotes
Mitosis is the replication of eukayotic cells that doesnt involve gametes - Interphase: this is where cells spend 90% of their time, with cells growing and chemical reactions occurring, and metabolic activity. During this phase the chromosomes replicate but do NOT condense and are still present in loosely packed chromatin, the centrioles also duplicate forming 2 pairs that line up just out side of nucleus - Prophase: in prophase there are 2 stages early prophase and late prophase. In early chromosomes begin to condense and become visible as loose X-shapes (sister chromatids joined by centromere). In late (prometaphase_ the nuclear membrane fragments, chromosomes condense furher and the microtubels of mitotic spindle invade there area of the nucleus to connect to the kinetochore on centromere region - Metaphase: the centriole pairs move to opposite ends of the cell, the chromosomes convene to the metaphase plate, centromeres are aligned and the chromosomes lie with long aces at roughly right angles o the spindle, then microtubels of spindles connect to centromere - Anaphase: this is where chromtids move apart, with each crhomatid. The spindle pulls on the chromosomes towards poles (end of cell) and at the same time poles of the cell move futher apart. - Telophase: the polar fibres extend the cell further and the daughter nuclei begins to form from the fragments of the parent nuclear membrane, cleavage furrow forms and the nucleoli reappears and the chromatin fibre uncoils - Cytokinesis: where the division of the cytoplasm occurs along the cleavage furrow which pinches the cell in two.
76
What is glycolysis
A metabolic process where glucose is broken down under anerobic conditions through a series of small steps through the metabolic pathway in the cytoplasm, where a specific enzyme catalyses each step, with intermediate compounds produced by enzymes. In glycolysis 2 ATP molecules are used to turn glucose into 2 pyruvates which causes 4 ATPs to be produced, and a net gain of 2 as 2 ATPs were used in the start of reaction.
77
What is the energy pathway of glycolysis
Each step in glycolysis is regulated, with each step being catalysed by a specific enzyme. In each step there is an intermediate compound produced that is converted to the next intermediate compound . Thus the step conversion of glucose to pyruvate enables the cell to control the process, minimising rapid reactions and maximise amount of energy stored in ATP molecule in the process.
78
What is a diploid cell
Two chromosomes of the same type make up an homologous pair, a cell that contains homologous chromosomes os called diploid cell.
79
What is a haploid cell
Cells that have only one chromosomes from each homologous pair.
80
What is asexual reproduction in eukaryotes
it is the propagation of an organisms without fertilisation, meaning genetically identical copies of the parent cell is produced, with it done through mitosis
81
Describe the formation of ATP from ADP and Pi
ATP synthesis primarily occurs in the mitochondria, with a small amount in the cytoplasm in eukaryotes, where an inorganic phosphate is added to APD with the energy to add the third phosphate group coming from respiration. It can be summerised by this equation: APD+ Pi + energy ------> ATP
82
Describe the conversion of ATP to ADP and Pi which releases energy for some metabolic reactions
ATP molecules have three phosphate groups therefore when reacted with water a process called ATP hydrolysis occurs with the bond between the second and third phosphate group breaking to form APD and Pi plus a net energy that the cell uses to work. It can be summarised by this equation: ATP ------> ADP + Pi + energy
83
describe the phases of meiosis in eukaryotic cells
in meiosis there is two consecutive cell division called meiosis 1 and meiosis 2. Meiosis 1 this is a reduction processes where its purpose is to halve the chromosome number. - Interphase: there is a pair of homologous pairs in the diploid parent cell, the chromosomes are duplicated, with now there being a pair of duplicated homologous chromosomes with sister chromatids, causing a diploid cell with duplicated chromosomes. - Prophase: the nuclear envelope is broken and the chromosomes begin condensing and each pairs with it homolog. The homologs align themselves gene by gene and allow crossing over to occur, where the dna molecule of nonsister chromtids are broken by an enzyme causing fragments to rejoing and form new combinations of alleles. At the same time centrosomes begin producing spindle fibres that attach to the centromeres of each homolog. Metaphase 1: pairs of homologous chromosomes are arranged at the metaphase plate through independent assortment. both chromtids of the homolog are attached to spindle fibres at opposite ends. Anaphase 1: the homologs move toward opposite ends of the cell Telophase 1: chromosomes move further apart and the nuclear membrane reforms from the parent cells fragments Cytokinesis 1: a cleavage furrow forms that pinches the cell into 2, creating 2 haploid daughter cells. Meiosis 2 - prophase 1: spindle apprapatus forms, chromosomes duplicate with each still composed of two sister chromatids. - Metaphase 2: the chromosomes are positioned at the metaphase and the centromeres are attached to spindle fibres from opposite ends - Anaphase 2: the sister chromatids separate and move toward opposite ends of the cell - Telophase 2: the nuclei forms and the chromosomes begin decondensing - Cytokinesis: the cell divides into two forming four haploid cells
84
explain why products of meiosis are haploid cells and contain a single set of chromosomes
when combined with other gamete through random fertilisation the diploid number is restored
85
why is crossing over and independent assortment important
increase genetic variation which significant in evolution when conditions in environment change genetic variation means more chance of offspring surviving, if all the same variation all will either survive or not
86
how is the diploid number restored
through random fertilisation
87
compare the products of meiotic and mitotic cell division
meiosis produces four cells and reduces the number of chromosome set from diploid (2) to haploid (1), produces genetically different cells from parent cell and each other Mitosis produces 2 daughter cells that are genetically identical to parent and another, diploid number stays the same
88
why is there more genetic variation in the products of sexual production compared to asexual production
89
describe the stages in cell cycle (include checkpoints)
4 phases G1, G2, S = interphase M = mitosis and cytokinesis - G1: cell is growing and carrying out cell functions - G1 checkpoint: checks for cell growth, DNA damage, and that the cell has the resources it needs - S: DNA is replicated - G2: further preparation for cell division and cell growth - G2 checkpoint: checks for cell growth, DNA replicated correctly and cell has the nutrients it needs - M: mitotic division and cytokinesis - M Checkpoint: checks at metaphase for correct chromosome alignment and spindle attachment
90
role of hormones in regulating cell division
- hormose is released by a signal cell and attaches to specific cell membrane receptors which contain a complementary #D shape to hormose on a target cell bidning leads to transcription and translation within cell to produce gene product that stimulates or inhibits cell division
91
explain the effect of carcinogens on cell division
affects the cells ability to participant in cell cycle correctly as they disable the checkpoints meaning now growth factors are required to move from one checkpoint to the next, and in some cases cancer cells have the ability to secrete growth hormones which cause blood vessels to direct over to them away from healthy cells
92
Describe 3 techniques of cell culture
SPRAY ON SKIN: healthy sample of skin cells from individual are collected and immersed in an enzyme solution to form a suspension liquid. The liquid includes keratinocytes, fibroblasts and melanocytes, which are cell types that are involved in wound healing. Then the suspension liquid is sprayed onto the burn wound, providing even and broad distribution of living cells across whole of wound. - VACCINCE PRODUCTION: using cell lines to manufacture vacines such as influenza vaccine to test for drug efficancy, optimal dosage and side effects - CANCER RESEARCH: looking at gene controll, changes in cell structure and function over time
93
discuss the limitations and applications of the contemporary examples of cell culturing
Limitations - cost - time consuming - contamination Advantages - millions grow in small space - detect mutations and the organisms carrying them - study mutant cells lacking enzyme - avoid ethical issues
94
What is the krebs cycle
Then the products from glycolysis go through the Krebs Cycle in the mitochondria, with enzymes again catalysing each step, and intermediate compounds produced. The Krebs cycle allows further breakdown of pyruvate while also releasing CO2 as a byproduct.
95
what is electron transport chain
This cycle then results in the oxidative phosphorlyation electron transport where electrons are transferred across electron transport chain and water is produced as a byproduct.
96
Effect of cyclin on mitosis
an increase of cyclin stimulates mitosis as the MPF increases stimulating mitosis through prophase and metaphase
97
what regulates cell cycle
two proteins involved: cyclin and cdk - cdk is a specifically an enzyme protein and cyclin binds to cdk at active site - different forms of cyclin bind to cdk at cell cycle, thus different cyclin rise and fall throughout cell cycle. The rising and falling will determine if the cell moves to next cell cycle phase
98
what happens if cell doesnt meet checkpoint requirements
if the reason can be fixed cell pauses in the phase until has the resources to fix issue if not cell undergos apoptosis
99
what causes uncontrolled division
a change in DNA sequence in a specific gene called protoncogenes which can turn into oncogenes. oncogenes cause cancer as cell division accelerates, which can be passed on in subsequent generations
100
what regulates cell cycle progression
Cyclins, Cdks and tumour suppressors
101
what is the role of cyclin in cell regulation
a protein that flucates in concentration to regulate progression in cell cycle phases. An increase in cyclin levels stimulates mitosis by forming the mitosis promoting factor
102
What is the mitiosis promoting factors (MPF)
a complex of proteins including cyclin and Cdk that triggers cell progression from the G2 phase to M phase
103
How does an increase in cycle levels affect mitiosis
an increase in cyclin levels leads to the formation of more MPF, the MPF stimulates mitosis through the prophase and metaphase
104
Three conditions necessary for successful cell culturing
sterile conditions to prevent infection of cells by microorganisms temperature - ensure enzyme working to opyimal effeciency to maximise rate of enzyme controlled reactions for max cell growth Nutrients - sugars amino acids as they are the basic building clocks for cellular growth and reproduction
105
explain how independent assortment contributes to variation
during meiosis 1 homologous pairs line up independently on other pairs, these separate into new combinations of maternal and paternal chromosomes, creating variation
106
Explain how crossing over contributes to variation
it is the reciprocal exchange of segments of genetic material of nin sister chromatids, this results in maternal and paternal genes to mix leading to new combinations of genes in the gamest produced thus causing varaiation in offspring
107
differences between binary fission and mitosis
spindle formed in mitosis not in binary fission chromosomes attach to spindle in mitosis but attach to cell membrane in binary fission nuclear membrane formed in mitosis linear chromosomes in mitosis and signular circular chromosome in binary fission
108
explain how the inhibition of cellular respiration would effect active transport
cellular respiration is the breakdown of glucose to release energy that is used to resynthesis ATP, which is the immediate source of energy for the cell for processes for active transport. Thus the inhibition of cellular respiration means less ATP production and therefore decrease in active transport
109
how is the energy stored in ATP released for use
ATP converted to APD and phosphate, the breaking of the bonds between last two phosphates releases energy used for cellular respiration
110
why is less energy released in anerobic respiration
because in aerobic respiration glucose in almost completely broken down into carbon dioxide and water releasing large proportions of energy from the breaking of chemical bonds, whereas in anerobic respiration glucose is partially broken down into lactic acid or ethanol therefore it releases smaller quantity of energy