Cells And Proteins- Lab Techniques Flashcards
What is the uses for linear dilution?
To work out the volume of stock to dilute with water to achieve a certain concentration
What is the method for linear dilution?
Using different volumes of the same stock solution and combining with different volumes of a suitable solvent
What can paper and thin layer chromatography be used for?
Separating different substances such as amino Addis and sugars
What is the method for log dilutions?
This is usually achieved by using successive dilutions as the new stick solution
These techniques use sticks of antibodies with the same specificity, known as…
Monoclonal antibodies
When is western blotting used?
After SDS-PAGE electrophoresis
What happens if the solution is buffered to a specific pH?
Only the proteins that have an IEP of that pH will precipitate
What can be separated in electrophoresis?
Proteins using their IEP
How can proteins be identified?
Using specific antibodies that have reporter enzymes attached
What is an antibody specific to the protein antigen linked to?
A chemical label
What is bright field microscopy used to view?
The whole organism, parts of organisms or thin sections of dissected tissues
What can microbial cultures be started with?
An inoculum of microbial cells on an agar medium
What are animal cells grown in?
A medium containing growth factors from serum
What is the difference between primary cell lines and tumour cells?
Primary- can divid a limited number of times
Tumour- can perform unlimited divisions
What does the plating out of a liquid micro oral culture on solid media allow?
The number of colony forming units to be counted and the density of cells in the culture estimated
What is serial dilution needed for?
To achieve a suitable colony count
What does vital staining do and how?
Identifies and counts viable cells by colouring dead cells ONLY
