Cells and Organisation Flashcards
What type of cells are animal and plant cells?
Eukaryotic cells
What are prokaryotes?
single-celled organisms
What are the 5 subcellular structures of an animal cell?
Nucleus, cytoplasm, cell membrane, mitochondria, ribosomes
What are the 3 subcellular structures that plant cells have but animal cells don’t have?
Cell wall, permanent vacuole and chloroplasts
What is the function of the nucleus?
contains genetic material that controls the activities of the cell
What is the function of the cytoplasm?
gel-like substance where most of the chemical reactions happen, contains enzymes that control the chemical reactions
What is the function of the cell membrane?
Holds the cell together and controls what goes in and out of the cell
What is the function of ribosomes?
where proteins are made in the cell (protein-synthesis)
What is the function of the mitochondria?
Where aerobic respiration takes place, it transfers energy that the cell needs to work
What is the function of the cell wall?
Supports and strengthens the cell
What is the cell wall made of?
cellulose
What is the function of the permanent vacuole?
It contains cell sap, a weak solution of sugar and salts
What is the function of chloroplasts?
Where photosynthesis takes place, which makes food for the plant. Contains chlorophyll that absorbs light for photosynthesis.
What do the chloroplasts contain?
Chlorophyll
What type of cells are bacteria?
prokaryotic cells
What do eukaryotic cells have the prokaryotic cells do not?
Nucleus
What do prokaryotes have instead of a nucleus?
a single circular strand of DNA that floats freely in the cytoplasm
What are plasmids?
small rings of DNA
What does bacteria not have that animal or plant cells have?
Chloroplasts or mitochondria
How do light microscopes work?
They use light and lenses to form an image of a specimen and magnify it. They let us see individual cells and large subcellular structures.
How do electron microscopes work?
They use electrons to form an image. They have higher magnification than light microscopes.
What are the differences in light microscopes and electron microscopes?
Light microscopes
- Use light and lenses
- lower magnification
- lower resolution
- less expensive
Electron microscopes
- Use electrons to form images
- higher magnification and resolution
- expensive
- requires training to operate
What is meant by resolution?
The ability to distinguish between two points, a higher resolution gives a sharper image.
What is the formula for magnification?
magnification = image size/ actual size
Give the method for the RP on microscopy.
Equipment:
- Light microscope
- Onion
- A slide (glass or plastic)
- Iodine solution
- Add a drop of water to the middle of a clean slide.
- Cut up an onion and separate it out into layers. Use tweezers to peel off some epidermal tissue.
- Place the epidermal tissue into the water on the slide.
- Add a drop of iodine solution.
- Place a cover slip on top.
- Observe using the microscope.
Why is iodine solution used in microscopy?
It is a stain, makes it clearer for objects in a cell to be seen.
Why is the cover slip placed down at an angle?
To reduce air bubbles obstructing the view of the specimen.
What are the 6 main parts of a microscope?
Eyepiece
Coarse adjustment knob
Fine adjustment knob
Light
Stage
High and low power objective lens
Explain how to use a light microscope.
- Clip the slide prepared onto the stage.
- Select the lowest-powered objective lens (lowest magnification)
- Use the coarse adjustment knob to move the stage up to just below the objective lens.
- Look down the eyepiece, use the coarse adjustment knob to move the stage down until the image is in focus.
- Adjust the focus with the fine adjustment knob, until you get a clear image.
- To see the slide in greater magnification, swap to a higher powered objective lens and refocus.
How should you draw your observation?
- Sharp pencil
- No colouring or shading
- Subcellular structures should be in proportion
- Include title and magnification
- label features using straight, uncrossed lines
What is differentiation?
The process by which a cell changes to become specialised for its job.
When does differentiation typically occur in animals?
As the organism develops but is lost at an early stage.
True or false : Lots of plant cells don’t ever lose the ability to differentiate.
True
What are cells that differentiate in mature animals used for?
Repairing and replacing cells
What are undifferentiated cells called?
Stem cells
Give 3 examples of specialised cells in animals?
Sperm cells
Nerve cells
Muscle cells
How are sperm cells specialised?
Long tail- to help swim
Streamlined head- to help swim
Lots of mitochondria- provide energy
Enzymes- helps digest through egg cell membrane
How are nerve cells specialised?
Long- cover more distance
Branched connections- to connect nerve cells and form a network
How are muscle cells specialised?
Long- so have space to contract
Lots of mitochondria- provides energy needed for contraction
Give 3 examples of specialised plant cells?
Root Hair cells
Phloem
Xylem
How are root hair cells specialised?
Large surface area- absorbs more water and mineral ions from the soil
What are embryonic stem cells?
Stem cells found in human embryos that can differentiate into any type of cell.
What are adult stem cells?
Stem cells found in adults, often in bone marrow. However, can’t turn into any type of cell, only certain cells like blood cells.
What is the difference between embryonic stem cells and adult stem cells?
Embryonic stem cells can differentiate into any cell, adult stem cells can only differentiate into certain cells.
What can stem cells be used for?
Can be grown in a lab to produce clones and made to differentiate into cells used for medicine or research.
What is therapeutic cloning?
An embryo could be made to have the same genetic information as the patient, so wouldn’t be rejected by the patient’s body.
What are the risks of using stem cells in medicine?
If grown in a lab, they could be contaminated and could pass on disease to the patient.
Why are some people against stem cell research?
- Religious beliefs that human embryos shouldn’t be used for experiments as each one is a potential human life
- In some cases, embryos that aren’t used are destroyed
- Scientists should focus on developing other types of stem cells
Where are stem cells found in plants?
meristems
How do stem cells work in plants?
They can differentiate into any type of plant cell throughout the plant’s entire life. They can produce clones quickly and cheaply. They can be used to grow rare plants to reduce risk of extinction. Can be used to create identical plants with desired features in farming.
Where are chromosomes found?
The nucleus of cells
What are chromosomes?
coiled up lengths of DNA molecules.
What do genes control?
The development of characteristics
How many copies of a chromosome do body cells have?
Two
How many pairs of chromosomes are in a human cell?
23
What are the two stages of the cell cycle?
1- Growth and DNA replication
2 - Mitosis
What does the cell cycle make cells for?
Growth, development and repair
In what stage of the cell cycle do cells divide?
Mitosis
What is produced at the end of the cell cycle?
Two new cells, identical to the original cell with the same number of chromosomes.
What happens during the growth and DNA replication stage of the cell cycle?
The DNA is spread out in long strings. Before it divides it must grow and increase the amount of subcellular structures such as mitochondria and ribosomes. It then duplicates its DNA so there is one copy for each new cell. The DNA is copied and forms X-shaped chromosomes. Each ‘arm’ of the chromosome is an exact duplicate.
What happens during mitosis?
- Once DNA has been copied, the chromosomes line up at the centre of the cell and cell fibres pull them apart.
- Each ‘arm’ of the chromosome goes to opposite ends of the cell.
- Membranes form around each of the sets of the chromosomes, creating a nuclei of the two new cells. (Nucleus divides)
- The cytoplasm and cell membrane divides.
- Two new daughter cells are produced. They are identical and contain the exact same DNA.
How do prokaryotic cells divide?
Binary fission.
How do prokaryotic cells replicate by binary fission?
- The circular DNA and plasmid(s) replicate.
- The cell gets bigger and the circular DNA strands move to opposite ends of the cell.
- The cytoplasm begins to divide and new cell walls begin to form.
- The cytoplasm divides and two daughter cells are produced. Each cell has one copy of the circular DNA but can have variable copies of the plasmids.
A bacterial cell has a mean division time of 30 minutes. How many cells will it have produced after 2.5 hours?
2.5 x 60 = 150 minutes
150/30 = 5 divisions
2^5 = 32 cells
Explain how to grow bacteria in a lab.
- Make an agar plate by pouring hot agar jelly into a petri dish.
- When the jelly is cooled and set, inoculating loops can be used to transfer microorganisms to the culture medium. Alternatively, a sterile dropping pipette and spreader can be used to get an even covering of bacteria.
- Place a lid over the top and store upside down in a lab at around 25°C.
What are bacteria grown in?
A culture medium
What does a culture medium contain?
The carbohydrates, minerals, proteins and vitamins that the bacteria need to grow.
Give 2 examples of culture mediums.
A nutrient broth solution or solid agar jelly
What can be seen when bacteria grow on agar jelly?
Colonies on the surface of the jelly or will spread out to give an even covering of bacteria.
Why are cultures of microorganisms not kept above 25°C in a school lab?
Harmful pathogens are more likely to grow above this temperature and grow a lot faster at higher temperatures.
What can you do to make sure that cultures are not contaminated?
- Petri dishes and culture medium must be sterilised before use (heat to high temp), to kill unwanted microorganisms.
- Inoculating loop must be sterilised by passing through hot flame.
- After transferring the bacteria, the lid must be taped on to stop microorganisms in the air getting in.
- The petri dish should be stored upside down to stop drops of condensation falling onto the agar surface.
Explain how you can test the effect of antibiotics on bacterial growth.
- Place paper discs soaked in different (types or concentrations) of antibiotics on an agar plate containing an even covering of bacteria. Leave some space between disks.
- The antibiotic should diffuse into the agar jelly.
- Use a control by placing a paper disc that has not been soaked in antibiotic but soaked in sterile water onto the agar jelly.
- Leave the plate at 25°C for 48 hours.
- The more effective the antibiotic is, the larger the inhibition zone will be.
What is an inhibition zone?
When no bacteria is growing.
How do you measure an inhibition zone?
πr^2
What is a bacterial colony?
groups of bacteria on a surface
What is diffusion?
Diffusion is the spreading out of particles from an area of higher concentration to an area of lower concentration.
Complete the sentence: Diffusion happens in both __________ and __________ because the particles are free to ___________ ___________.
solutions, gases, move, randomly
Complete the sentence: The bigger the concentration gradient the _________ the diffusion rate.
faster
What is a concentration gradient?
The difference in concentration
Give an example of diffusion.
Small molecules such as oxygen, glucose, amino acids and water diffuse through cell membranes.
Complete the sentence: The __________ the surface area, the faster the diffusion rate.
larger
What is osmosis?
Osmosis is the movement of water molecules across a partially permeable membrane from an area of high water concentration to an area of low water concentration.
What is a partially permeable membrane?
A membrane with small holes that only tiny molecules can pass through.
Explain the effect of sugar solutions on plant tissue (potato). RP
IV- Concentration of sugar solution
DV - Change in mass
CV- surface area of potato, volume of solution, time in solution, temperature, type of sugar
- Cut up a potato into equal cylinders.
- Fill some beakers with equal volumes of different sugar solutions. One should be pure water and another should be a very concentrated sugar solution. The rest should be in between.
- Measure the starting mass of each cylinder.
- Leave a cylinder in each beaker of solution for 24 hours.
- Take out of the solution and pat dry.
- Measure the mass of the cylinders and calculate the % change.
If the potato cylinders increase in mass, what has happened?
Water has moved in by osmosis.
What is active transport?
Diffusion against a concentration gradient, moving from a lower concentration to a higher concentration.
What does active transport require?
energy
Explain how root hair cells take in minerals and water.
The concentration of minerals is usually higher in a the root hair cell than in the soil around them. Minerals are absorbed by the root hair cells by active transport from an area of low mineral concentration to an area of high mineral concentration.
Give an example of active transport in humans.
Making glucose from the gut and kidney tubes.
Complete the sentence: How easily something moves between an organism and its environment depends on its __________ ________ to ___________ _______.
surface area, volume, ratio
True or false : The smaller an organism is, the smaller its surface area to its volume.
False - The larger an organism is, the smaller its surface are to its volume.
What are exchange surfaces?
Surfaces that are adapted to maximise the exchange of a substance.
Which type of organism has a smaller surface area to volume ratio?
multicellular organisms
How are exchange surfaces adapted?
- Thin membrane (short distance to diffuse across)
- Large surface area (lots of substance can diffuse at once)
In animals : - lots of blood vessels to get stuff in and out od the blood fast
- gas exchange surfaces are ventilated so air can move in and out
Explain how gas exchange happens in the lungs.
The lungs contain millions of little air sacs called alveoli where gas exchange takes place.
Alveoli have a huge surface area, moist lining, thin walls and a good blood supply to maximise the diffusion of oxygen and carbon dioxide.
In the alveoli, carbon dioxide from the blood capillary diffuses into the alveolus whilst oxygen diffuses into the blood stream.
What gases are involved in gas exchange in the lungs?
Carbon dioxide and oxygen.
Where does gas exchange take place in the lungs?
in alveoli which are attached via bronchiole.
What are villi?
Tiny projections inside the small intestine that increase the surface area so digested food is absorbed much more quickly into the blood.
How are villi adapted for their function?
- Single layer of surface cells
- very good blood supply to assist quick absorption
Explain gas exchange in leaves.
The underneath of a leaf is its exchange surface. It contains little holes called stomata which carbon dioxide diffuses through. Oxygen and water vapour also diffuse out through the stomata.
The size of the stomata is controlled by guard cells that close the stomata if water is being lost faster than it is being replaced. The flattened shape of the leaf increases its surface area so that gas exchange is more effective.
What is the size of stomata controlled by?
Guard cells
Explain how gills work.
- Water (containing oxygen) enters the fish through its mouth and passes out through the gills.
- Oxygen diffuses from the water into the blood in the gills and carbon dioxide diffuses out of the blood and into the water.
- Each gill is made up of thin plates called gill filaments that have a large surface area for gas exchange.
- The gill filaments are covered in tiny structures called lamellae, which increase the surface area.
- The lamellae have lots of blood capillaries to speed up diffusion.
- They also have a thin surface layer to minimise the distance that the gases have to diffuse.
- Blood flows through the lamellae in one direction, water flows in the opposite. This maintains a large concentration gradient.
- The concentration of oxygen is always higher in the water than blood.