Cells and cell membranes Flashcards
resolution of a light microscope
200nm
resolution of a TEM
0.1 nm
resolution of a SEM
0.1nm
magnificent of a light microscope
x1,500
magnification of a TEM
x500,000
magnification of s SEM
x100,000
magnification
the degree to which the size of an image is larger than the object itself
resolution
the degree to which it is possible to distinguish between two objects that are very close together
explain the need for staining for use in light microscopy and electron microscopy
a lot of biological material inside the cell isn’t coloured, so it might be difficult to distinguish between different features.
coloured stains are used to stain specimens for use with the light microscope.
chemicals which bind to other chemicals in or on the specimen allow the specimen to be seen
some chemicals bind to specific structures e.g. acetic orcein staying dna red
electron micrographs start off B&W with the colour being added by a specialised computer program afterwards.
linear magnificent of an image
image size= actually size x magnification
nucleus
largest organelle
houses all the cell’s genetic material in the form of DNA, which contains the instructions for protein synthesise.
nucleolus
dense spherical structure inside nucleus
makes ribosomes and RNA which pass into the cytoplasm and are used in protein synthesis
nuclear envelope
surround the nucleus
double membrane with nuclear pores
SER and RER
continuous with the nuclear envelope. RER is studded with ribosomes, SER is not
transports proteins made by the attached ribosomes
SER involved in making lipids
golgi apparatus
stack of membrane bound flattened stacks
modifies proteins received from the rough ER and then packages them into vesicles so they can be transported