CELLS Flashcards

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1
Q

What is the structure and function of the cell surface membrane?

A
  • The cell surface mebrane is made up of a phospholipid bilayer
  • Each phospholipid has a hydrophobic fatty acid tail which is found on the inner side of the membrane so that cells do not separate out with water and a hydrophyllic phosphate head
  • Controls what enters and exits the cell, contains various transport proteins which facilitate the movement of substances in and out of the cell, e.g. carrier and channel proteins
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2
Q

What is the structure and function of the nucleus?

A
  • nucleolis: production of ribosomes- mRNA and tRNA
  • nuclear envelope: double membrane, can contain ribosomes on its surface
  • nuclear pores: allows the passage of large molecules in and out of the cell
  • nucleoplasm: site for biochemical reactions in the production of ribosomes, also stores ions and molecules
  • nuclear membrane: in places iit is continuous and joins twith the endoplasmic reticulum
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3
Q

What is the structure and function of the mitochondria?

A
  • Cristae: extensions of the inner membrane- increases SA:V ratio
  • Matrix: stores proteins, lipids, ribosomes and DNA
  • Produces ATP for the generation of energy
  • Contains ribosomes to make its own proteins
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4
Q

What is the structure and function of chloroplasts?

A
  • chloroplast envelope- double plasma membrane
  • Grana: stacks of up to 100 disk like structures called thylakoids, thylakoids contain chlorophyll and they have tubular structure which join up with thylakoids in adjacent granas. Granas are the site of light absorption,m the first stage of photosynthesis.
  • Granas membranes have large SA for the attachment of chlorophyl, electron carriers and enzymes, for the efficient absorption of light
  • Stroma: fluid filled matrix and is the site of sugar synthesis, the second stage of photosynthesis, it contains starch grains for energy and the proteins needed to synthesise sugars
  • Chloroplasts contain both DNA and ribosomes so they can manufacture proteins needed for photosynthesis
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5
Q

What is the structure and function of the golgi apparatus and golgi vesicles?

A
  • A stack of membranes that make up flattened sacs called cristernae with small rounded hollowed shapes called vesicles
  • Proteins from the ER are sent here to be modified and labelled, so they can be transported to the right place
  • Modified proteins are then transported through vesicles which fuse with the cell membrane to release its contents- exocytosis
  • adds carbohydrates to proteins to form glycoproteins
  • transport modifies and stores lipids and proteins
  • secretes carbohydrates
  • forms lysosomes
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6
Q

What is the structure and function of lysosomes?

A
  • lysosomes are formed when vesicles from the golgi apparatus contain the hydrolytic enzyme lysozyme, which can hydrolyse cell walls
  • lysosomes isolate these enzymes before releasing them to phagocytal cells
  • Autolysis- completely break down dead celld
  • hydrolyse the cell walls of cells that have been ingested by phagocytal cells
  • digest worn out cells so their chemicals can be reused
  • Release lysozymes to the outside of a cell so that it can destroy whats around it
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7
Q

What is the structure and function of ribosomes?

A
  • Larger in eukaryotic cells that prokaryotic
  • 2 sub units both containing RNA and protein
  • site of protein synthesis
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8
Q

What is the structure and function of the endoplasmic reticulum?

A
  • sheet like mebranes that spread through the cytoplasm of the cell
  • Rough ER: has ribosomes on its outer surface. It provides a large SA for the synthesis of proteins and glycoproteins. Also provides a pathway for transporting material especially proteins around the cell
  • Smooth ER: lacks ribosomes on its outer surface. It is used for the transport, storage and synthesis of carbohydrates and lipids
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9
Q

What is the strusture and function of the cell wall?

A
  • Cellulose: Made up of bonds between Beta glucose to form a straight unbroken chain, these chains then bond via hydrogen bonds to form microfibrils and macrofibrils
  • Hydrogen bonds provide the cell wall with structural support, which is necessary for keeping the plant cells turgid
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10
Q

What is the structure and function of a cell vacuole?

A
  • Tonoplast- the mebrane surrounding the vacuole, controls what enters and leaves
  • Vacuolar sap: contains water, ions, sugars, enzymes, pigments and waste products
  • Keeps the plant upright by maintaining turgor pressure
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11
Q

Describe the fluid mosaic model?

A
  • movement of the phospholipids and associated protein membranes means that small uncharged molecules can freely enter the cell
  • phospholipid bilayer- hydrophobic fatty acid tail and hydrophyllic phosphate head
  • cholesterol: when cold stops fatty acid tails sticking together to maintain fluidity, and when too hot stops tails from pulling apart to keep the membrane partially permeable
  • Integral proteins: channel proteins and carrier proteins
  • polysacharides, glycolipids and glycoproteins necessary for cell signalling/antigens
  • Extrinsic protein: anchors to the cykoskeleton to keep it from moving around
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12
Q

What is simple diffusion?

A
  • net movement of particles down a concentration gradient requiring no external energy. The only particles that can pass throught the phospholipid bilayer via simple diffusion are small and uncharged
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13
Q

What affects the rate of movement across cell membranes

A
  • surface area- offers opportunity for more proteins on the cell surface membrane
  • carrier and channel proteins- affects the rate of facillitated diffusion
  • gradients and water potential- affects the rate of diffusion
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14
Q

What is facillitated diffusion(deeply)?

A
  • carrier proteins bind to specific molecules, causing its shape to change and the molecules to be released on the other side of the membrane.
  • Channel proteins are water filled pores which span the membrane they allow the transport of specific molecules, depending on size property and charge.
  • The maximum rate occurs when all proteins are in use.
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15
Q

What is osmosis?

A
  • the net movement of water particles down a concentration gradient through a partially permeable membrane
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16
Q

What is active transport(deeply)?

A
  • Movement of particles against a concentration gradient, requires external energy
  • The hydrolysis of ATP releases a phosphate group which binds to a carrier protein and changes it shape
  • Sodium potassium pump: 3 sodiums leave the cell and 2 potassiums enter
  • Hydrogen pump: responsible for transporting sucrose into phloem’s companion cells
  • Vesicle movement: ATP required to move myosin (motor protein) which is responsible for transporting vesicles around the cell
17
Q

How can cells be adapted for rapid transport across their membranes?

A
  • increased surface area gives an opportunity for increased number of channel and carrier proteins, which increase the rate of transport as more molecules can be transported at one time
  • A steep concentration gradient increases the rate of diffusion and a high water potential increases the rate of osmosis
18
Q

True or False? Prokaryotic cells are much smaller than eukaryotic?

A

TRUE

19
Q

What does a prokaryotic’s cell cell wall contain?

A

Murein- a glycoprotein

20
Q

Are the ribosomes in a prokaryotic cell smaller than in a eukaryotic?

A

Yes

21
Q

What is the difference between the cytoplasm of a prokaryotic cell to a eukaryotic cell?

A

Prokaryotics have cytoplasms lacking membrane bound organelles

22
Q

What do prokaryotics have instead of a nucleus?

A

single circular DNA molecule, that is free in the cytoplasm and is not associated with proteins

23
Q

True or False? viruses are a-cellular and non-living

A

TRUE

24
Q

What is the basic structure of of virus particles?

A

ALL:
- have nucleic acid in the centre, a capsid which is a protein coat that encases the nucleic acid and attachment proteins to allow viruses to attach to a host cell
SOME:
- have a lipid envelope which was stolen from a host cell

25
Q

What are the principles and limitation of an optical microscope?

A
  • The light beam is focused through a lens
  • relatively cheap
  • max magnification 1500x
  • max resolution 0.2 microm
  • Staining of the specimen is required
  • you can veiw a live specimen
  • 2-D image
26
Q

What are the principles and limitation of a TEM?

A
  • Electrons are the source of the image
  • expensive
  • max magnification 50 million
  • max resolution 1-20 nm
  • electrons have shorter wavelengths and therefore produce higher resolution
  • The image is 2-D
  • a live specimen can not be used
27
Q

What are the principles and limitation of an SEM

A
  • Electrons are the source of the image
  • expensive
  • max magnification 15 million
  • max resolution 1-20 nm
  • electrons have shorter wavelengths and therefore produce higher resolution
  • The image is 3-D
  • a live specimen can not be used
28
Q

What is the formula for magnification?

A

Magnification= image size/actual size

29
Q

What are the principles of call fractionation and ultracentrifugation when used to seperate the components of a cell?

A

Stage 1:
- homogenisation
Tissue is placed in a cold (reduce enzymatic activity), buffered(constant pH), isotonic (equal water potential) solution. Solution is put in a homogeniser to break down cell surface mebranes, then filtered to remvoe mebranes, and then homgenate is placed in a test tube
Stage 2:
- Ultracetrifugation
Test tube is placed in centrifuge, which is then spun on the lowest speed causing the heaviest organelles to float to the bottom(pellet) and the rest to the top(suptanant), so that they can be seperated. The supertanant is then put back in the centrifuge and spun on the next highest speed, so that the next organelles will float to the bottom, this step is thgen repeated.
- Order of pellets
1. nucleus
2. Mitochondria/ chloroplast
3. lysosomes
4. ER
5. ribosomes

30
Q

How do you use idodide potassium to identify starch grains in a plant cell?

A
  • Carefully peel a thin layer of onion with tweezers
  • place this on a glass slip
  • Add a drop of potassium iodide
  • Use a mounting needle to slowly lower a cover slip on top
  • place the glass slip on the stage and start with the lowest magnification, using the coarse adjustment knob to bring it into focus
  • Then switch to the next magnification and use the ifne adjustment knob to focus it
  • The iodine should have stained the starch grains
31
Q
A