Cells Flashcards
What is active transport?
Carrier proteins bind to molecule./ion to be transported
-molecule binds to receptor sites on carrier proteins
On inside of cell ATP binds to protein causing it to change shape to allow molecule into cell
ATP splits into ADP and phosphate
After molecule is released phosphate is released from proteins which means it reverts back to original shape
What is facilitated diffusion?
Facilitated diffusion is passive
Larger, water soluble molecules have to be taken across plasma membrane by carrier proteins or protein channels
Carrier proteins facilitate movement by physically binding molecules on one side of the membrane and releasing them on the other
protein channels allow ions through
Disadvantages of electron microscopes?
- Complex preparation (staining)
- Preparation kills living (Vacuum)
- More likely image contains artefacts
TEM only
- Specimen must be extremely thin
- Image only in 2D
Disadvantages of light microscopes?
- Can only see certain objects
- resolution is limited by light wavelength
What is endoplasmic reticulum (ER)?
Sheet like membranes - flattened sacs and tubules
Inside ER is fluid spaces and cisternae
Rough ER:
- Has ribosomes on surface (where proteins are synthesised and stored)
- Provides large SA for synthesis of proteins and glycoproteins
- Provides pathway for transport of materials especially proteins throughout cell (Vesicles)
Smooth ER:
- Doesn’t have ribosomes on surface
- Synthesises, stores and transports lips, phospholipids and steroids
- Synthesises, stores and transports carbohydrates
What is a TEM?
Transmission electron microscope
- Uses a beam of electrons instead of light -electrons have much smaller wavelength
- Beam passes through thin slice of specimen prepared in a vacuum
- denser parts of specimen are darker (absorb more electrons)
- Very high resolution allows us to see structure of cell in very high detail
What is an SEM?
Scanning Electron Microscope
- Beam of electrons is directed at surface of specimen
- Beam bounces off surface of specimen and pattern of scattering electrons allows a 3d image
- Slightly worse resolution than TEM
- Doesn’t need to be as thin as TEM
Stages of HIV?
- HIV binds to CD4 receptor on T helper cells
- Lipid envelope fuses with cell membrane of T helper cell
- HIV RNA and reverse transcriptase is released into T helper cell
- Reverse transcriptase turns RNA into DNA
- HIV DNA is inserted into T helper cell DNA -whenever T helper cell divides, it copies HIV DNA . HIV DNA remains inactive for long time
- HIV DNA eventually becomes activated resulting in new HIV proteins and RNA (Lots of HIV particles in helper cell)
- New HIV is released from cell (which dies) and then infects new T helper cell
What is Golgi Apparatus?
- Consists of flattened sacs (cisternae) with small rounded hollow vesicles
- Proteins and lipids produced by the ER are passed through Golgi in a strict sequence
- Modifies proteins, sorts and packages them into Golgi vesicles which are regularly pinched off
What is functions of Golgi?
- Add carbohydrates to proteins to form glycoproteins
- Produce secretory enzymes
- Secrete carbohydrates, such as those used in making cell walls
- Transport, modify and store lipids
- Form lysosomes
What is cell wall?
Consists of microfibrils embedded in a matrix
Consists of a number of polysaccharides
Middle lamella marks boundary between adjacent cell walls / cements cells together
What is function of Cell wall?
- Provides strength to stop cell bursting under osmotic pressure
- Provides mechanical strength to plant as a whole
- Allows water to pass along it and so contribute to movement of water through the plant
What is a lysosome?
Vesicles formed by Golgi that contain enzymes - lysozymes
Lysozymes -enzymes that hydrolyse cell wall of certain bacteria
what is function of lysosome?
- Hydrolyse material ingested by phagocytes
- release enzymes to outside of cell to destroy material around the cell
- Digest worn out organelles so that chemicals they’re made of can be reused
- Completely breaks down cells after they have died (autolysis)
Structure of viruses?
extremely small
have nucleic acid core (DNA or RNA) with outer protein capsid
some:
- Lipoprotein envelop outside of capsid
- Receptors
- Enzymes