Cellmembranes and transport Flashcards

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1
Q

Phospholipids structure.

A

Phosphate head - polar - hydrophylic - soluble in water

Fatty acid tail - non-polar - hydrophobic - insoluble in water.

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2
Q

What is a phospholipid monolayer?

A

where Phospholipids spread over the surface of water and form a layer

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3
Q

Phospholipid bilayer also contains protiens. Name the two types and explain

A

Intrinsic embeded in the membrane - arrangement determined by their hydrophilic and hydrophobic regions

Extrinsinc on the outer or inner surface of the membrane

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4
Q

What is the fluid mosaic model?

A

Cell membranes are fluid because the phospholipids and protiens can move around via diffusion

mosaics because the scattered pattern produced by the protiens within the phospholipid bilayer looks somewhat like a mosaic from above

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5
Q

Cholesterol in the cell surface membrane.

A

cholesterol molecuels have a hydrophobic tail and hydrophylic head

fit between phospholipid molecules and oriented the same way (head out, tail in)

are absent in prokaryotes membranes.

restrics the movement of other molecules making up the membrane

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6
Q

Glycolipids in cell surface membrane

A

lipids with carbohydrate chains attached.

chains project out into whatever fluid is surrounding the cell. (found in the outer phospholipid monolayer)

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7
Q

Glycoprotiens use in the cell surface membrane

A

Protiens with carbohydrate chains attached. chains project out into whatever fluid surrounds the cell, found in the outer phospholipid monolayer

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8
Q

what are protiens which span the entire membrane called? with one example.

A

Transmembrane protiens
e.g. transport protiens

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9
Q

Facilitated diffusion, why needed, what uses it and what assists it?

A

Some substances cannot diffuse through the phospholipid bilayer.

Large polar molecules e.g. glucose and amino acids.

Ions such as sodium and chloride

Channel protiens and carrier protiens

highly specific, only allow one type of molecule or ion to pass through.

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10
Q

Channel protiens

A

water filled pores

allow charged substances to diffuse

not free, gated, protien inside moves to open and close the pore

this allows controled exchange of ions.

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11
Q

Carrier protiens

A

unlike channel protiens, they can switch between two shapes

this causes the binding site to pe open to one side of the membrane but not the other and then switch

net movement of particles will occur down a concentration gradient of the specific molecule.

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12
Q

How does a plant retain water and what happens if it can not?

A

Water will enter the plant cell through the partially permeable cell surface membrane by osmosis.

This is due to the higher water potential outside the cell.

Water enters the vacuole - incrase in plant cell volume.

Protoplast (living part of the cell inside the cell wall) pushes against the cell wall and pressure builds up inside the cell.

inelastic cell wall prevents bursting

The pressure createrd by the cell wall also stops too much water entering and this also helps to prevent the cell bursting

becomes fully turgid, rigid.

turgidity provides support and strength for the plant - stand upright with leaves held out to catch sunlight.

Without enough water, the cells can not remain rigid and firm and the pland wilts.

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13
Q

What happens if a plant cell is placed in a solution with lower water potential.

A

plant cell placed in concentrated sucrose solution

water will leave the plant through the partially permeable membrane by osmosis.

As water leaves the vacuole of the plant cell, volume decreases.

Protoplast gradually shrinks and no longer exerts pressure on the cell wall.

it now begins to pull away from the cell wall.

this is Plasmolysis

the plant is plasmolysed

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14
Q

What is a hypertonic solution?

A

Red blood cells have higher water potential than solution

net movement of water out

flacid cells.

any concentrated solution (sucrose)

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15
Q

What is an isotonic solution?

A

Water potential is equal between the red blood cell and solution

No net movement water.

Normal cells (healthy)

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16
Q

Hypotonic solution

A

Red blood cells have lower water potential than solution.

Net movement of water in

cells swell may burst (turgid)

e.g. pure water

17
Q

Osmosis plant vs animal

A

Both have phospholipid bilayer
both are partially permable.

Only plant cell has cell wall (cellulose and fully permeable)
Animal cell does not

Osmosis can happen both ways in both types (in and out)

Cell placed in solution with lower water potential:

animal : water leaves through cell surface membrane
vol of cell decreases
cells shrinks

Plant: water leaves through cell surface membrane.
vol of cell decreases.
Protoplast shrinks
cell is plasmolysed

Higher conc:
animal: water enter…
vol inc
no cell wall for pressure
streatched (eventual burst

Plant: same stuff
protoplast expands/ push against cell wall, fully inflated and turgid now.

18
Q

Where does the energy for active transport come from?

A

respiration

19
Q

what is energy required for in active transport?

A

To make the carrier protien change shape allowing it to transfer ions/molecules across the cell membrane.

20
Q

What are the uses of active transport?

A

Reabsorption of useful molecules and ions into the blood after filtration into the kidney.

Absorption of some products of digestion from the digestive tract

loading sugar from the photosynthesising cells of leaves into teh phloem tissue for transport around the plant

loading inorganic ions from the soil into root hairs.

21
Q

Co-transport definition

A

coupled movement of substances across a cell membrane via a carrier protien.

22
Q

Absorption of glucose

A

Co-transport

Sodium ions and glucose molecules are transported into the epithelial cells via facilitated diffusion

This can only continue if the concentration gradient is maintained.

Active transport of sodium ions out of the cell into the blood maintains this gradient.

The glucose molecules exit the epithelial cells and enter the blood via facilitated diffusion.

23
Q

Rate of diffusion factors:

A

Simple:
surface area of exchange surface
concentration gradient across exchange surface
Thickness of exchange surface

Facilitated:
Concentration gradient across exchange surface
number of channel or carrier protiens available in the exchange surface.

24
Q

Speciallised cells for diffusion

A

Root hair cells.
specialliesed shape, root hair, increases the surface area so rate of water uptake by osmosis is greater.
(more water and mineral ions)

thinner walls than other plant cells
water can move through easily

permanent vacuole containing cell sap
more concentrated than soil water
high water potential gradient is maintained.

Epithelial cells in small intestine:

microvilli.
increased durface area for greater rate of diffusion
constant blood supply maintains a high concentration gradient between lumen and epithelial cell.

25
Q

speciallised cells for facilitated diffusion

A

Neurones and muscle cells.

Cell membranes that contain channel protiens for sodium, potassium and calcium ions.

The opening and closing of channel protiens results in facilitated diffusion and he number of the channel protiens increases the speed of electrical transmission in both nerve impulses and muscle contraction.

26
Q

How can you test membrane fluidity?

A

beetroot
dark purple pigment
higher the permeability, the more pigment leaks out

equal sized cubes of beetroot 5 samples
hot water bath and heat at different temps
remove and measurewith colorimeter

higher temp means phospholipids not as tightly packed together
increases the permiablity of the membrane

lower than 0 degrees celcius

ice crystals can pierce the ecell membrane making it more permeable

remember the usual deforming of channel protiens for both