Cell Theory, Microscopes, Prokaryotes and Eukaryotes Flashcards
Outline the functions of life
Metabolism, reproduction, sensitivity, homeostasis, excretion, nutrition, growth/movement
State the cell theory
all cells come from pre-existing cells, cells are the smallest unit of life, all organisms are made up of cells
Outline basic structures in all cells
Plasma membrane- outer border of cell, Genetic material- cell DNA, Ribosomes- create cell proteins, Cytosol- cell internal fluids
Describe 4 atypical examples of cell theory
Striated muscle fibers, aseptate fungal hyphae, sieve tube elements, and red blood cells
Describe the basics of light microscopes
There are light and electron microscopes
Describe the two staining options for light microscopes
Fluorescent labeling and immunofluorescence staining
Describe the basics of electron microscopes
Scanning is 3D and transmission is flat or cross section
Describe cryogenic electron microscopes
Freezes samples without using heavy metals
Describe freeze fracturing
Cracks along a plane, this is how we see the cell membranes
What is the formula for magnification?
image/actual
Describe the structure of a prokaryote
Structured small and simple, no compartmentalization, circular DNA, and 70s ribosomes
What type of ribosomes are found in prokaryotic cells?
70s ribosomes
What type of DNA is found in prokaryotic cells?
Circular DNA
Describe the 2 domains that contain bacteria
Bacteria and Archaea
State the scientific and common shapes found in bacteria
Bacillus - rods, Coccus - spheres, Spirillus- spiral