Cell Structure Flashcards

1
Q

Define magnification

A

How much bigger an image is compared to a specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Define resolution

A

The ability to distinguish between two separate points

How detailed an image is

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the maximum magnification of a light microscope

A

X1500

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is the maximum magnification of a TEM

A

X>1,000,000

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the maximum magnification of a SEM

A

X<500,000

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is the maximum resolution of a light microscope

A

200nm/0.2um

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the maximum resolution of a TEM

A

0.2nm/0.0002um

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the maximum resolution of a SEM

A

20nm/0.002um

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Advantages of light microscope

A

Relatively cheap
Can observe live specimens
Colour images

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Disadvantages of light microscope

A

Low resolution (lower than electron microscopes)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Advantages of TEM

A

High resolution images to look at very small organelles eg ribosomes
Look at internal structures of organelles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Disadvantages of TEM

A

Specimens must be dead
Specimens must be thinly sliced
Images are black and white (colour can be added)
Produces 2D images

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Advantages of SEM

A

Produces a 3D image

Shows the surface of an organism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Disadvantages of SEM

A

Specimens must be dead
Lower resolution than TEM
Images are black and white (colour can be added)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Advantages of scanning laser confocal

A

Pinhole prevents out of focus light reaching the detector so image is clearer
Can view thicker and living specimens at different depths

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

How does a light microscope work

A

Uses a number of lenses to produce an image that can be viewed directly at the eyepiece.
Light passes from a bulb under the stage, through a condenser lens and then through specimen.
This beam of light is passed through an objective lens (x4, x10, x40) and then the eyepiece lens (x10)

Looks at whole cells or tissues

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

How does a TEM work

A

Uses electromagnets to focus a beam of electrons, which is transmitted through the specimen.
Denser parts of the specimen absorb more electrons, which makes it look darker on the image, creating contrast

Produces 2D images

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

How does a SEM work

A

Scan a beam of electrons across the specimen

This knocks off the electrons across the specimen, which are gathered in a cathode ray tube to form an image

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

How are specimens treated before viewing with a SEM

A

Coated with a thin layer of gold particles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

How are specimens treated before viewing with a LCSM

A

Tagged with fluorescent dye

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

What is the function of the pinhole in LSCM

A

Prevents out of focus light reaching the detector

22
Q

What does methylene blue stain and it’s colour

23
Q

What does giemsa stain and it’s colour

A

Blood cells
Red blood cells stain red
White blood cells stain purple

24
Q

What does eosin stain and it’s colour

A

Cytoplasm

Pink

25
What does haemotoxylin stain and it’s colour
RNA purple | DNA blue
26
Why are specimens often stained before viewing under a light microscope
Organelles are often transparent so it prevents object from appearing white
27
How would you calculate actual size of a magnified object
Actual = image/ magnification
28
How does a LSCM work
Uses laser beams to scan a specimen, tagged with fluorescent dye Laser beam is focused through a lens which is aimed at a beam splitter Beam splits and some light is directed to specimen When the laser hits dye it gives off fluorescent light Light if focused through pinhole onto a detector Detector is hooked to a computer which generates the image
29
How to prepare sample for use with electron microscopes
Treat it with heavy metals (like lead) | Metal ions scatter electrons foraged at the sample and give contrast between different structures
30
How to prepare a dry mount
Use a thinly sliced specimen Use tweezers to place specimen in the middle of a clean slide Place a cover slip on top
31
What can a dry mount be used for
Observing hairs, Parts of insects Pollen Parts of a flower
32
How to prepare a wet mount
Pipette a small drop of water onto the slide Use tweezers to place specimen on water droplet Stand cover slip upright near water droplet Carefully tilt and lower slip so it covers the specimen Try to avoid air bubbles Add a stain to edge of cover slip, drawing the stain across the specimen
33
What can a wet mount be used for
Living samples eg tiny aquatic organisms
34
How to use a light microscope
1. Clip the slide containing the specimen onto the stage 2. Select lowest powered objective lens 3. Use coarse adjustment knob to bring stage up to just below objective lens 4. Look down eyepiece, using coarse adjustment knob to move stage until image is in focus 5. Adjust focus with fine adjustment knob until you get a clear image 6. Swap to higher powered objective lens if required
35
What is an eyepiece graticule
It is fitted onto the eyepiece Like a transparent ruler with numbers but no units When you look through eyepiece you’ll see a scale
36
What is the stage micrometer
Placed on the stage It is a microscope slide with an accurate scale (has units) Used to work out value of divisions on the eyepiece graticule at a particular magnification
37
Why are a variety of stains used to stain samples
Different stains can be used to make different parts of cell show up
38
Structure and function of nucleus
Large organelles surrounded by nuclear envelope (double membrane) Contains many pores Contains chromatin (made of DNA and proteins) Contains a nucleolus Controls cells activities by controlling transcription of DNA DNA contains instructions to make proteins Pores allow substances to move between nucleus and cytoplasm Nucleolus makes ribosomes
39
Structure and function of plasma membrane
Made of lipids and proteins Regulates movement of substances in/out of cell Has receptor molecules which allow it to respond to chemicals like hormones
40
Structure and function of cell wall
Rigid structure that surrounds plant cells Made of cellulose Supports plant cells
41
Structure and function of lysosome
Round organelle surrounded by a membrane with no clear internal structure Contains digestive enzyme To digest invading cells or break down worn out components Transports hydrologic enzymes
42
Structure and function of rough ER
System of membranes enclosing a fluid filled space Surface is covered with ribosomes Folds and processes proteins made at the ribosomes
43
Structure and function of the smooth ER
System of membranes enclosing a fluid filled space Synthesises and processes lipids and steroid hormones
44
Structure and function of a vesicle
Small fluid filled sac in the cytoplasm surrounded by a membrane Transports substances in/out of cell via plasma membrane Some are formed by Golgi apparatus or ER and some at the cell surface
45
Structure and function of Golgi apparatus
Group of fluid filled membrane bound flattened sacs Vesicles at the edges of the sacs Processes and packages new lipids and proteins Makes lysosomes
46
Structure and function of mitochondrion
Double membrane Inner membrane folded to form cristae Inside is the matrix which contains enzymes for respiration Site of aerobic respiration where ATP is produced Found in large numbers in cells that are active and require a lot of energy
47
Structure and function of chloroplast
Small flattened structure found in plants Surrounded by double membrane Membranes inside called thylakoid membranes Thylakoid are stacked up to form grana Grana are linked together by lamellae - thin flat pieces of thylakoid membrane Site of photosynthesis Some photosynthesis happen in grana and other parts in the stroma (thick fluid in chloroplasts)
48
Structure and function of a centriole
Small hollow cylinders made of microtubules (tiny protein cylinders) Found in animal cells but only some plant cells Involved in separation of chromosomes during cell division
49
Structure and function of cilia
Small hair like structures found on the surface membrane of some animal cells Cross section - outer membrane and a ring of 9 pairs of protein microtubules inside and a single pair in the middle Microtubules allow cilia to move Movement is used by the cell to move substances along the cell surface
50
Structure and function of flagellum
Flagella on eukaryotic cells are like cilia but longer Stick out from the cell surface Surrounded by plasma membrane Two microtubules in centre and 9 pairs around the edge Microtubules contract to make flagellum move Flagella propel cells forward (eg when sperm swims to egg)