Cell structure Flashcards
What is resolution?
The ability to distinguish two objects as separate from each other
Allows details to be see with clarity.
It’s determined by the wavelength of light or electron beam.
What is magnification?
The number of times an image is greater than the object.
It increases the ability to see details
State the resolution and magnification that can be achieved using a light microscope.
Magnification: x1500
Resolution: 200 micrometers
State the resolution and magnification that can be achieved using a transmission electron microscope.
Magnification: x500,00
Resolution: 0.1 nm
State the resolution and magnification that can be achieved using a scanning electron microscope.
Magnification: x100,000
Resolution: 0.1nm
Explain how samples are stained in a light microscope.
Uses a machine with metal knife to cut thin sections
Then placed on a glass slide
Uses dyes e.g iodine, methyl blue, acetic orcein
Explain how staining is used in TEM
Uses salts of heavy metals to absorb electrons after it has been sectioned using a diamond knife. It’s placed on a copper grid with a mesh.
Why do you need to stain samples?
It is necessary to give contrast between structures.
Stains are taken up differently by objects with different densities
What is the equation to calculate actual image size?
Actual image size = image size/magnification
Units must always be in micrometers!!!!
1mm = 1,000 micro metres 1cm= 10,000 micrometers
State the function and structure of the plasma membrane
Made of lipids and proteins and creates the outer surface of animal cells.
Used in cell signalling and regulates the movement of substances
State the function and structure of the cell wall
Made from cellulose network
Rigid structure that surrounds plant cells and prokaryotes and supports cell
State the function and structure of the centriole
Small hollow cylinder containing microtubules
Involved in separation of chromosomes in cell division
State the function and structure of the nucleus
Large organelle surprised by nuclear envelope contains many pores
Contains chromatin and often a structure called nucleolus
Pores allow RNA to move between the nucleus and the cytoplasm
State the function and structure of the lysosomes
A round organelle surrounded by a membrane with no clear internal structure
Contains digestive enzymes
Kept separate from the cytoplasm by the surrounding membrane
State the function and structure of the ribosomes
A very small organelles that floats free in the cytoplasm or is attached to RER
Site where proteins are made